LOCUS       sequence-42931-e        5547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCherry-ABCC5a
FEATURES             Location/Qualifiers
     source          1..5547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..722
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(160..178)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     428..447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5100..5234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5124..5143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5178..5197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5347..5367)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5347..5365)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5386..5402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5386..5402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5399..5421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5410..5426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5434..5440,5441..5458,5459..5464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5479..5500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctagtcatcg attgatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
       61 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      361 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      481 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      541 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      601 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      721 aggaattcgg agccggcgcg ccattaatta aaatgattat agaaggcaac gatcctctca
      781 gtatgacgtc accacaccgt gcatcagagg gcgatataca caatgatgaa gggtttgggg
      841 tgcaagagag gagttcctca ttagaagatc aaactgtcat cgaaatggat tcacagattg
      901 acacggctct gagctacacc gacgggaaga cacccggaga actgaaagac gggagaatag
      961 gagagcagga ggatgaccca gatgaaactg aacaacttct ggacaaacgg gaagaaggtg
     1021 atactgaaga acagaagagt agcaacaccg gaactaaata ttgggcgacc ggtaacttca
     1081 tcagcgtcgt aacgtcgcag tggttaacac cgctgttccg cgccgccaag aaacgcggac
     1141 taaacgacga cgacctctat cacatcttgc ccgtggactc agcggagaaa aacgccaaga
     1201 tctttgctca gttatgggag gaagagataa aacatcacgg tggtaacgcg gtcaaagcgt
     1261 cactgagacg cgtcattctg cggttcgtat cgtggcgtat tcttggctgt atgctcatgg
     1321 ttatgttaag ccagatgacc gtatttcttc tttcctccgt ttctatccgt ggccttctcc
     1381 aatattccgc ggatgcgact gcgcccgttt accgcggtat tctgtacgcg gtcagcgcca
     1441 tgctcttcac gattctctac aacattaccc tcggtgcaaa ctacttcatc gcggctagca
     1501 ccggggtgcg gctgcgaggc ggtgtggtgg cgcttttact gcagaaggtt ctgcggctgc
     1561 gtggtcttca caagacatcg atgggtgaga ttgtgaatct atgtgttaac gacgggaaca
     1621 gactccatga tggaatggtg tttacacctt tggtcgttgg ggcgcccgtg atattcattt
     1681 gcgcatgcgt attcacgact tacatcttgg gcaaatcagc tttgattggg atgttggtct
     1741 acgcgctaac atatattgca cagttcgctc taacaggagg taaggtgatc taccgtgcac
     1801 gcatgatgaa agagacagat caacgagttc acaccatcag tgagatgctg actaacatca
     1861 agttcatcaa gatgtatgca tgggaagatt gcttcgagag gaaggtcaat gaactccgcc
     1921 gaagggaact acgacatcta gttggtttgc gcattcttca tggtatccaa aagagcttca
     1981 acatttttgt tccaatagta gctgttgtgg ccacaattat gttcaagctc tttctgaaag
     2041 agagtctttc cccagatcag gtgttgacct atttagctgt catctacagc atgcgttcga
     2101 tgctcaccat gactcagtat ggtttccaat acttgagtga tatcctggtg tccataccac
     2161 gtatcaagaa cttgctgatt caagatgaga tgctacccat cacggatagt gtcgatgact
     2221 ggtcgaatgc gatcgagatg aactcggtgt cggctgcttg ggatgactgc agtaaagaca
     2281 agaagactca atgcgataca gctcgggata aagaagatca tgacgtcatt gacagagaaa
     2341 atggttcgtc gtcagacctc tatggagcat gcgcattgaa tgatgtatcg gttgcagtaa
     2401 aaaagggatc tcttgttggt gtatgcggta gcatagggag tggtaaaacc agcttactac
     2461 acaccatact acaacagaca cacatcctag aagggtctat agccgtatca gggtctttgg
     2521 catacacctc ccaacaacct gatatcttca acgcaacatt acgtgagaac atcaccatgt
     2581 tcggcgacca ttgtgaagat gaagtcgaga atgagaacca agctgagaga gttcacagtg
     2641 aagagagata ctgggaagcg gtcagggttt gttgtctgag tgacgacatc gcggcgcttc
     2701 catgcggtgg tcgtacggag atcggcgagc gtggtatcaa cctgagtgga ggacagaagc
     2761 atcgtgtgag tctggctcga gctgtgtacg ctgatcgaga catctactta ctggatgatc
     2821 cgttgagtgc agtggataca agggtagcag aacacatcta cacagagtgc atcgcaggta
     2881 tcctcaagga gaagaccgta ctcttagtca caaccaacct acagtacctt agcaaatgtg
     2941 atgaggtgat tgttatcagc aatggacggg tgatcgaaca gggtacacac acgcagctta
     3001 tgacagatga agccgagtat gctcgccttc tacgaacatt tggctctgac cacgacaaaa
     3061 cagcagacga ctacatcagc aagcagaacg aagacgaaag caccgataac gatgacctct
     3121 tggcaaattg ctcgacgaga actctgaatg aagaggactg tctagatgaa aacattgagg
     3181 gtcagttggt ggacgcagag acccaaaagc agggtgcagt cagcacaggg acctacctgg
     3241 cctatttcaa atacgcagga ggcatacttt catgtttcct ttctttactc ttgtatattc
     3301 tatcaccagc aagtgctgca ttcaacaacg tctggcttag tcattggctc agcgcgggca
     3361 gtggcattcc tcaaaatggc acccagctac agtcagaaga tgaacaagat attctgaaga
     3421 atcctgaatt ccactttttc agggatgtct atctttatag ctttctgagt gtgtttgtaa
     3481 tatgtagtct tcgtatagtt tattatgtaa tgattacgca gagggcgtcg ttcaaccttc
     3541 atcgatccct ctacaagcag gtcttcagtt gcccgatgga attctttgat accactccat
     3601 cgggaagggt cctgaatcga ttctctcgtg acattgatga agcagataat gaagtcccga
     3661 acgctctcga gacggccctc tggacatttc tcagtgtgtt tgcaggtatt gtaattgtca
     3721 tcatggtctt tccctggttt atcgtctttg ttgccatcat tgctattgtc cttgtgtatt
     3781 tgtctcgaat gtttctacca gctattcgtg atatcaaacg tattgaaaac atcaaacgat
     3841 caccactcat cggacacgtt aaagctacag tcgaggctca gaccaccatc cgggcatttg
     3901 gcatgggaga actcttcatt aaacgctgta acaagctctt agacaccagc gctgttccaa
     3961 agatgctatt tttaatggga caacgatggt tggcattcag actggaccta tgcgtagcag
     4021 tagcccaact gctacttggt ctatctgtcg tttttacaca caacactact gctgccgctt
     4081 ttgccggact ggctcttgct tactccgtac agttcctagg gacacttcaa ttttctgttc
     4141 gacttatcac gatcacacag gcccgtttca catccattga acgcattacc gattatatca
     4201 aaagattgat accagagggg ggcaccggga aaaagcatat attaaaaagt gatgtggaga
     4261 gttggcccaa acatggaaat atcacagttg attcacttca actcaaatat cgagacaacc
     4321 ttccgactgt gttgaaaggt atttctttcc atgtcaattc taaagagaaa attggaatcg
     4381 ttggaagaac tggatcaggc aaatcgtcca ttgcagtagc cctatttcgt ctcgtcgaag
     4441 caacctctgg cagaatccat atcgatgacc tcgacattgg gagcatccgt atcagggagc
     4501 ttcgttcacg tatctccgtg attcctcagg atcccgttct ctttacagga actcttcgtt
     4561 tcaacctaga tccatacgaa cagtactccg gcgctcagat ttgggatgct ttagataaaa
     4621 catacatgaa aaaaagcatt gctggtatcc agcagcaact tgaagtccgg gtgagggaaa
     4681 atggctctaa cttttccact ggagaaagac aactgctctg tctagcaaga gctctcctgc
     4741 gcaactctac gatactgttc cttgacgaag cgactgcgac tgttgatgct gaaacagaca
     4801 gtctggtcca aagtacaatc agaacagctt tttcaaactg cacagttctg accatagccc
     4861 atcgtcttca cacagtccta gactctgacc gtattcttgt cttagatgat ggtaccgtcc
     4921 tggagatgga tactccagca gcacttcttg ccaacccaga ctccagacta aacgctctct
     4981 tgagctcagg acatgacgtt tcagtcaatc aaaccaacca ttgtggacac aattagtggc
     5041 cggccagcga tcgccaaggc ctctcgagcc tctagaacta tagtgagtcg tattacgtag
     5101 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5161 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5221 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     5281 tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt
     5341 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     5401 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga gcataaagtg
     5461 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5521 cgctttccag tcgggaaacc tgtcgtg
//
