LOCUS       sequence-42932-e        6200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmCherry-ABCC9a
FEATURES             Location/Qualifiers
     source          1..6200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4827..5537
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4972..4990)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5240..5259
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5588..5722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5612..5631)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5666..5685
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5835..5855)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5835..5853)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5874..5890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5874..5890)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5887..5909)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5898..5914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5922..5928,5929..5946,5947..5952))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5967..5988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6105..6122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctagtggcgc gccaatggga tcagatgatt gggagtggtt ctgtggtaca aacagcagtg
       61 acctgccaga ctttgataac cagtcttgga actatgagga tgtgttgagt aatgagtgtt
      121 tggtggatgc tttcatcgcc gggactcaga tagggttctt cgtcctagct cttctagttc
      181 tctttctcac cgcatgctgc acctcacgca gtgatgtacc acatcggttc ctgctccgtt
      241 acgtaacaca tagtcctaga tatcttggta cctttgttct ggtcgtgatg tcagtggcat
      301 ctgttgcgga aggtatcctg actgatagca catataccaa ccaacctaca caaccacatc
      361 tctacattac aggcatatca tctgcgttaa gtggagttgt atccctgctc tactttggtc
      421 gtctcgagag atggcgtgtt tggaagatgt ctctcctcct cctcgtctac tgggtggctt
      481 ccttggcaac cgtaagcatc cagattgagt ccctcatcca tcagggatac aacgatgttc
      541 atgtgacagc cttcgacatc aatatcatca tgattgcagt atacagcttc ttcttactcc
      601 tcgagctgta tttcctcgta gacaactggt ttggttggag tcccggcgtg gatgaagtgc
      661 ctcgagactt aaaggatgaa cgcatgtact acatggagcg ctacgtcgac atcttctctc
      721 agcttgtcca ttggtggatg aactggctct tcgttctcgg tttcaagagg ccactcgaga
      781 tggatgatct gggaagcata ccggaggtcc acagcgccga gtacaatcat agacggttca
      841 agaacaactt caagagagag atagagcggg caaaggaaga agagcgggaa ccgagtttgt
      901 tcaaggtgta ttttgccacc tactgggtcc gaatgctggc cggtgctatc ctcaagttca
      961 tcacagacat gggagcctat gtgggtccgt tggccattgg aggcatcact ctttatgtca
     1021 ccgatgctgt ctacggtgaa catgaagacg taccacccca cccggtgagc gtaaaagagt
     1081 tcttcgcaga tggttacgtt ctgatcggtt gtatattcgt cactgctctt ataagacaca
     1141 tgtgtgacca aacctatcag tttatcacct ccatagaagg catccatgct aggtcggccc
     1201 ttcagagtac gatctacgag aaatccttac gactgtctac atacgccatg actggagggg
     1261 cgatgaccat gggacagatc accaatcaca tgtcagtaga tgccaacaat atcatgatgt
     1321 tcttcatgtt tgctaacgaa gtctgggcaa ctccgttcaa gatcatattt tccttggtac
     1381 tgttatacct tgagctgggt acaccagcat tgatcggtgc cagtgtcttt ctagttgtga
     1441 tccctttcca actagctgct gccgtcgcta tgggaaaatt catcaaggct gttttgggta
     1501 cggcggacta ccgtctgaag atgtcaaacg agatgctaca gggaatcaaa ctcttgaagc
     1561 tttatggatg ggagaggata tactatgaag caatcaagaa ggcgagaatc aaggaacttt
     1621 atgctcttct aaaggtctac acattcttcg ctgtaacgtt tatcatcaat gctgcaactc
     1681 caatcgtcgt cacccttgtg tctttcggga catacacacc catcacgggg aaggtcctca
     1741 cacctgacgt ggcatttaca acgctctcgc tcttcaatca aatggctggt cctctcttca
     1801 tgtttccttt cgtcatcaat ctctttgtca actggatcgt gagtacagga cgtcttaaga
     1861 cgtatctcct tgcacccgag gttgaaggcg ccgatggagt gatcagcgat ctactgaagg
     1921 agattgatga ggatggcgat gtaacggaca gtgccgagag cgtgggtaat tcacacgatg
     1981 acgatctttc ggtgaccatc aagcatcccc ggcacaggaa gaaccgaagt catagcaaac
     2041 agagagacga tgctcatcaa ctccctgata agacaccatt gttgagcggg gacggatcgc
     2101 cacgaggatc tctctcttac ggatctacca atcccatcaa acatggagat acgtcattca
     2161 gtggcagcgg ggatgtcccc gatgacgtcg ccataaagat ctctgatggt aacttcactt
     2221 gggatcctga ttccaatata tctatactgt ccgacatcaa tgtagagata ccaagaggta
     2281 agctaacagt cgtgattggg gcggttggat gcggaaaatc atcccttctc agtgccatgt
     2341 tgaatgaaat gacaacactc tccggcaccg ttaagcacaa cagaaacacg aaatccattg
     2401 catacgcccc acagaaagca tggttggtca atgctacgct caaagacaac atattatttg
     2461 gcgaagagat ggttaacagc aaatatcgca aagtgatcca agcatgcgca ctgcagcccg
     2521 acatagacat gttacctggt aaagacatga cagagattgg cgagaaaggg atcaacctga
     2581 gcggagggca gaagcaacgc gtcagtgtca gccgatgtct ctactccgac agggacatca
     2641 tcatcttgga cgaccctctc tctgctctgg acatgcacgt tggcacgcat ctgttcgaga
     2701 aaggattcca aggtatcttg atgaaacaaa agaagacagt catactggta acccatcagc
     2761 tccagtatct gcctgaagcc gacaaaatca ttgtggtgaa ggatggccgc atcacttgcc
     2821 agggtactcc cgaggaggta gccgaggctg acccttcgat catgactgag tctgagagag
     2881 ctatcaagga agtgaccgaa tccgaagccg aagggtctgg ggcagagtct gagtctctga
     2941 tgaatgagag aagggcgctc aagagacaga tctcaacaca gatgtctgtg ttgaaggacg
     3001 gcgctgctga taaatcagac gagaaagcag gacgcttgat tgaagaggag gagaaggaga
     3061 aaggttctgt gtcttaccag atgtacatgt actacttccg agccatgggc tattggcttg
     3121 catgtctcat gatcggttgt gtagccatgc gagccggtct tcaggtagct acaaacttct
     3181 ggctctcaga ctggtccgag gcgggtctca atttaactgg ggacgcccaa ccgacgagtt
     3241 attatttgag aggttatgcg ggtttctctg tgtctactgt ttttggatcc gttttatcga
     3301 atgtcttcag ctgtatcggt gccttgctag cggcaagagc gatacacttt gcccttctac
     3361 aaaatatcat cggaatacca atgagattct ttgacaccac cccagttgga cgaattctca
     3421 accgactctc cagtgacaca caatggatcg atcagcgttt gattcactcc ttcaacatgc
     3481 ttctcaatac gttagtcgcc gtcatcgcct cctttatcgt caacgccatc gtcaatagct
     3541 acttcatcat cttcctcatc cctatcggca tcatcttcat gtgcattctg gtctacttcg
     3601 tcgcaacatc cagaatgttg cagcgtttgg agagcgtcac aagatctcct gtgttcgctc
     3661 atttctctga aacgcttggc ggtctgccca tcatcagagc atataaggat gaaaagcggt
     3721 ttttcaaaaa cgtactgggg aaaatcgaca aaaacaatac agctttggtg tatttgtaca
     3781 cagccaacag atggttagcg attcgattgg attatctggg agcagtggtt gtgctcattg
     3841 ccagtctgtc ggcactcttg ggggcgctct atcttggtgt agatgctagt ttggttgggc
     3901 ttgccatcag ctattcatta gagatctcca tctatatgaa catggtggtg cgaggttgtg
     3961 ccgacttgga gcttcagatg aacgccatgg agagaattcg atactacact gaagtagaga
     4021 atgaggaata cgatggtctg gagcctccac ccgagtggcc ccagaaaggt gaaatcattc
     4081 ttgataacat cagcgtacgt tacgccctgg agctcgatcc tgtactgcat gatgtctctg
     4141 tcaccatccc tcctggtcaa aagttgggaa tctgtggtcg aaccgggagc ggtaaatcat
     4201 cgcttacact ttctctcttc cgtatcatcg atactttcaa aggtcgaatt ctgattgacg
     4261 gagttgatat ttcatcagtt cctctcatca cactccgtca acgactttct atcatcccac
     4321 aagatgccgt cctcttcact ggaactatca gacataacat tgatccagcg ggtgtaaaga
     4381 cagacgatga actatggaag gctttagaga tagctcaact caaagaagtc gtttcaccgt
     4441 tagaaggtgg attggactat atcgtgacgg agggaggaga gaatttcagc gttggtcagc
     4501 gacagctctt ctgtctggcc cgggctttcc tccgtaactc aactatcttg gtcatggatg
     4561 aagctactgc ttccatcgat caagaaactg atcgcatcct ccaagacgtg gtggctgatg
     4621 tcttccaaga tagaacagtc atcacaatcg cgcatcgcgt gggtacgatc ttggacgcag
     4681 ataacatcct cactctcagt gacggtcgcg tcgtcgagtt cgactccccg aacacgctcc
     4741 tcgaaaaaga agacagcatc ttcgcgtcgc tggtcaaagc tggcaaatta attaaaggcc
     4801 ggccagcgat cgccggacat cgattgatgg tgagcaaggg cgaggaggat aacatggcca
     4861 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     4921 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     4981 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     5041 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     5101 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     5161 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     5221 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     5281 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     5341 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     5401 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     5461 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     5521 acgagctgta caagtaagaa ttcaaggcct ctcgagcctc tagaactata gtgagtcgta
     5581 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     5641 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5701 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     5761 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
     5821 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     5881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc
     5941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6181 ttatccacag aatcagggga
//
