LOCUS       sequence-429342-        9553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 D10A+D839A+H840A+N863A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      693..702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(699..725,726..728,729..3212,3213..3218,3219..3284,
                     3285..3287,3288..4802)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4815..4835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4890..4909)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4934..4982
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5184..5612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5271..5290)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5481..5502
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5621..5641)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5626..5955
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5806..5941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5868..5887
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6022..6816
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6076..6095)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6686..6705
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6992..7113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7029..7048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7083..7102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7162..7178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7162..7178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7175..7197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7186..7202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7210..7216,7217..7234,7235..7240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7255..7276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7393..7410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7564..8152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7644..7663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8323..9114,9115..9183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGVRVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8946..8965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9184..9288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9363..9382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatcgaaccc ttgccaccat ggacaagaag tactccattg
      721 ggctcgctat cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc
      781 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca
      841 ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag
      901 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta
      961 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg
     1021 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt
     1081 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata
     1141 aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact
     1201 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac
     1261 tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg
     1321 ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac
     1381 agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc
     1441 tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt caactgagca
     1501 aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag
     1561 accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag
     1621 tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc
     1681 accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca
     1741 aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa
     1801 gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg
     1861 agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg
     1921 gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg
     1981 atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga
     2041 taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg atgactcgca
     2101 aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg
     2161 cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc
     2221 ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc accaaggtca
     2281 aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta
     2341 tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact
     2401 atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca
     2461 acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg
     2521 acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag
     2581 atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca
     2641 tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca
     2701 atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat
     2761 ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca
     2821 tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg
     2881 caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg
     2941 tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc
     3001 aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta
     3061 taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga
     3121 atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac
     3181 tggacatcaa tcggctctcc gactacgacg tggctgctat cgtgccccag tcttttctca
     3241 aagatgattc tattgataat aaagtgttga caagatccga taaagctaga gggaagagtg
     3301 ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga
     3361 acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc
     3421 tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca
     3481 ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca
     3541 aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca gatttcagaa
     3601 aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct
     3661 acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat
     3721 ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg
     3781 aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga
     3841 ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag
     3901 aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg aaggtcctgt
     3961 ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg
     4021 aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc
     4081 ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca
     4141 aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa
     4201 tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata
     4261 aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt gagcttgaaa
     4321 acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac
     4381 tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt
     4441 ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg
     4501 agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac gctaacctcg
     4561 ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa
     4621 acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg
     4681 acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac gccacactga
     4741 ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag
     4801 acagcagggc tgaccccaag aagaagagga aggtgtgaaa gggttcgatc cctaccggtt
     4861 agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
     4921 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     4981 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5041 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5101 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
     5161 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5221 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5281 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5341 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5401 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5461 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5521 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5581 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5641 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5701 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5761 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5821 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5881 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5941 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     6001 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6061 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6121 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6181 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6241 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6301 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6361 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6421 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6481 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6541 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6601 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6661 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6721 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6781 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat
     6841 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6901 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6961 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7021 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7081 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7141 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9061 tcgtacaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9301 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     9361 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     9421 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     9481 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     9541 agatatacgc gtt
//
