LOCUS       sequence-429355-       12486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor with P2A-human 
            P53DD and P2A-human MLH1dn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12486)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 12486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
FEATURES             Location/Qualifiers
     source          1..12486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     587..606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(825..929)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1008..1027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1199..1578
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1579..1782
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1733..1753
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1824..1843
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1824..1842
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1892..1912
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1913..6013
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6071..6091
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8192..8212
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8222..8248
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8258..8314
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8627..8683
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10948..10965
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10988..11005)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10994..11218
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11289..11305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11289..11305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11302..11324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11313..11329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11337..11343,11344..11361,11362..11367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11382..11403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11520..11537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11691..12279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11771..11790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12450..12486,1..755,756..824))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
       61 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      121 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      181 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      241 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      301 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      361 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      421 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      481 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      541 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      601 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      661 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      721 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      781 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      841 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      901 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      961 gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct
     1021 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     1081 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     1141 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     1201 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1261 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1321 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1381 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1441 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1501 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1561 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1621 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1681 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1741 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     1801 atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg ccaccatgaa
     1861 acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag tcgacaagaa
     1921 gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
     1981 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
     2041 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct
     2101 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
     2161 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
     2221 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
     2281 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
     2341 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
     2401 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
     2461 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
     2521 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagaa agctggaaaa
     2581 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
     2641 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
     2701 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
     2761 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
     2821 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
     2881 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
     2941 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
     3001 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
     3061 cggcaccgag gaactgctcg tgaagctgaa gagagaggac ctgctgcgga agcagcggac
     3121 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg
     3181 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct
     3241 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg
     3301 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa
     3361 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa
     3421 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct
     3481 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca
     3541 gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct
     3601 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga
     3661 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa
     3721 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac
     3781 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga
     3841 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg
     3901 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa
     3961 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt
     4021 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat
     4081 tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt
     4141 ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc
     4201 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat
     4261 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac
     4321 ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt
     4381 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggacgcta tcgtgcctca
     4441 gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg
     4501 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg
     4561 gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga
     4621 gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac
     4681 ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga
     4741 cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc
     4801 cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc
     4861 ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct
     4921 ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa
     4981 gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa
     5041 ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga
     5101 gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg
     5161 gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg
     5221 cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa
     5281 ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct
     5341 ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct
     5401 ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc
     5461 caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt
     5521 cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa
     5581 cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa
     5641 gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca
     5701 ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga
     5761 cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga
     5821 gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt
     5881 caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga
     5941 cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca
     6001 gctgggaggt gactccggcg gaagctctgg tggcagcaag cggaccgccg acggctctga
     6061 attcgagagc cctaagaaga aaagaaaggt gagcggaggc tctagcggcg gaagcaccct
     6121 gaacattgaa gacgagtata gactgcatga aacaagcaag gaacccgacg tgtccctggg
     6181 ctccacctgg ctgtccgact ttccccaggc ctgggccgag acaggaggaa tgggcctggc
     6241 cgtgcggcag gcacccctga tcatccctct gaaggccacc tctacacccg tgagcatcaa
     6301 gcagtaccct atgtctcagg aggccagact gggcatcaag cctcacatcc agaggctgct
     6361 ggaccagggc atcctggtgc catgccagag cccctggaac acaccactgc tgcccgtgaa
     6421 gaagccaggc accaatgact atagacccgt gcaggatctg agagaggtga acaagagggt
     6481 ggaggatatc caccccaccg tgcccaaccc ttacaatctg ctgtccggcc tgcccccttc
     6541 tcaccagtgg tatacagtgc tggacctgaa ggatgccttc ttttgtctga gactgcaccc
     6601 taccagccag ccactgttcg cctttgagtg gagggaccct gagatgggca tctctggcca
     6661 gctgacctgg acacgcctgc ctcagggctt caagaatagc ccaacactgt ttaacgaggc
     6721 cctgcaccgc gacctggcag atttccggat ccagcaccca gatctgatcc tgctgcagta
     6781 cgtggacgat ctgctgctgg ccgccaccag cgagctggat tgccagcagg gaacacgcgc
     6841 cctgctgcag accctgggaa acctgggata tagggcatcc gccaagaagg cccagatctg
     6901 tcagaagcag gtgaagtacc tgggctatct gctgaaggag ggccagagat ggctgacaga
     6961 ggccaggaag gagacagtga tgggccagcc aacacccaag accccaagac agctgaggga
     7021 gttcctgggc aaagcaggat tttgcaggct gttcatccca ggattcgcag agatggcagc
     7081 acctctgtac ccactgacca agccgggcac cctgtttaat tggggccctg accagcagaa
     7141 ggcctatcag gagatcaagc aggccctgct gacagcacca gccctgggcc tgccagacct
     7201 gaccaagcct ttcgagctgt ttgtggatga gaagcagggc tacgccaagg gcgtgctgac
     7261 ccagaagctg ggaccatgga gacggcccgt ggcctatctg tccaagaagc tggacccagt
     7321 ggcagcagga tggccaccat gcctgaggat ggtggcagca atcgccgtgc tgacaaagga
     7381 tgccggcaag ctgaccatgg gacagccact ggtcatcctg gcaccacacg cagtggaggc
     7441 cctggtgaag cagcctccag atcgctggct gtctaacgcc cggatgacac actaccaggc
     7501 cctgctgctg gacaccgatc gcgtgcagtt tggccctgtg gtggccctga atccagccac
     7561 cctgctgcct ctgccagagg agggcctgca gcacaactgt ctggacatcc tggcagaggc
     7621 acacggaaca aggccagacc tgaccgatca gcccctgcct gacgccgatc acacatggta
     7681 taccgatgga agctccctgc tgcaggaggg ccagaggaag gcaggagcag cagtgaccac
     7741 agagacagaa gtgatctggg ccaaggccct gccagcaggc acatccgccc agcgggccga
     7801 gctgatcgcc ctgacccagg ccctgaagat ggccgagggc aagaagctga acgtgtacac
     7861 agactccaga tatgccttcg ccaccgcaca catccacgga gagatctaca ggcgccgggg
     7921 ctggctgacc tctgagggca aggagatcaa gaacaaggat gagatcctgg ccctgctgaa
     7981 ggccctgttt ctgcccaagc ggctgagcat catccactgt cctggacacc agaagggaca
     8041 ctccgccgag gcaaggggca atcggatggc cgaccaggcc gccagaaagg ctgctattac
     8101 tgaaactccc gacacttcca ctctgctgat tgaaaactcc tccccttctg gcggctcaaa
     8161 aagaaccgcc gacggcagcg aattcgagtc tcccaagaag aagaggaaag tcggctctgg
     8221 ccctgccgct aagagagtga agctggacgg atccggcgca acaaacttct ctctgctgaa
     8281 acaagccgga gatgtcgaag agaatcctgg accgatggag cctcaccacg agctgccccc
     8341 agggagcact aagcgagcac tgcccaacaa caccagctcc tctccccagc caaagaagaa
     8401 accactggat ggagaatatt tcacccttca gatccgtggg cgtgagcgct tcgagatgtt
     8461 ccgagagctg aatgaggcct tggaactcaa ggatgcccag gctgggaagg agccaggggg
     8521 gagcagggct cactccagcc acctgaagtc caaaaagggt cagtctacct cccgccataa
     8581 aaaactcatg ttcaagacag aagggcctga ctcagacgga agcggagcta ctaacttcag
     8641 cctgctgaag caggctggag acgtggagga gaaccctgga cctagcttcg ttgctggagt
     8701 catccggaga ctggacgaga cagtggtgaa cagaattgcc gccggcgagg tgatccagag
     8761 acctgccaat gcaataaagg agatgatcga gaactgtctg gacgccaagt ccacaagcat
     8821 tcaggtgatc gtgaaggagg gcggactgaa gctgatccag atccaagaca acggcacagg
     8881 catcagaaag gaagatctgg acatcgtgtg tgaacggttc accacatcta agctgcagtc
     8941 ttttgaggat ctggcctcta tcagtaccta cggcttcaga ggcgaggccc tggccagcat
     9001 cagccacgtg gcccatgtga ccatcaccac caaaaccgcc gacggcaaat gcgcttatcg
     9061 cgctagctac agcgacggca agctgaaagc cccgccaaag ccttgcgccg gcaaccaggg
     9121 tacacagata acagtggagg atctgttcta caacatcgcc acccggagaa aggccctgaa
     9181 aaatcccagc gaggagtacg gcaagatcct ggaagtcgtc ggcagatact ccgtgcacaa
     9241 cgccggaatc agctttagcg taaagaagca gggagaaacc gtggccgatg tgcgcaccct
     9301 gccaaatgcc agcaccgtgg ataacatcag aagcattttc ggaaatgccg tgtccagaga
     9361 actgatcgag atcggctgcg aagataagac cctggctttt aagatgaacg gctacatcag
     9421 caacgccaat tactctgtga agaagtgcat ctttcttctg ttcatcaacc acagactggt
     9481 ggaaagcacc agcctgcgga aagccatcga gacagtgtac gccgcctacc tgcctaagaa
     9541 cacccacccc ttcctgtacc tgagcctcga gatcagccct cagaacgtgg acgtcaatgt
     9601 gcatcctaca aagcacgagg tgcacttcct gcacgaggaa agcatcctgg aaagagtgca
     9661 gcagcacatt gagagcaagc tgctgggctc taacagcagc agaatgtact tcacacagac
     9721 cctgctgcct ggcctggccg gcccctcagg cgaaatggtt aagtccacaa cctctctgac
     9781 ctcatccagc accagcggtt cttccgataa ggtgtacgcc caccagatgg tgcggaccga
     9841 ctctcgggag cagaagctgg acgcctttct gcaacctctg agcaaacctc tgagctctca
     9901 gcctcaggcc atcgtgaccg aggacaagac agatatctcc tccggccgtg ccagacagca
     9961 ggacgaagaa atgctcgagc tgccagctcc tgccgaggtg gccgccaaga accagagcct
    10021 ggagggagat accacaaagg gcaccagcga aatgagcgag aagcggggcc ctacctccag
    10081 caaccccaga aaacggcacc gggaggacag cgacgtggaa atggtggagg acgacagcag
    10141 aaaggaaatg acagccgctt gtacccctag aagaagaatc atcaacctga cctccgtgct
    10201 gagcctgcag gaggagatca acgagcaggg ccacgaggtg ctgagagaga tgctgcacaa
    10261 tcacagcttc gtgggctgcg tgaaccctca atgggccctg gctcagcatc aaacaaagct
    10321 gtacctgctg aacaccacca agctgagcga agagctgttc taccagatcc tcatctacga
    10381 cttcgccaac ttcggcgtgc tacgcctgag cgagcccgcc cctctgtttg acctggccat
    10441 gctggctctg gatagcccag aaagcggctg gacagaagag gacggaccta aagaggggct
    10501 ggctgaatac atcgtggagt tcctgaagaa aaaggccgag atgctggccg actacttttc
    10561 tctggaaatc gacgaggaag gcaacctgat cggcctgcct ctgctgatcg ataactacgt
    10621 gcctcccctg gaaggcctgc ccatcttcat cctgagactg gctacagagg tgaactggga
    10681 cgaggaaaag gaatgcttcg agtctctgag caaggagtgc gccatgttct atagcatcag
    10741 aaaacagtac atctctgaag agagcactct gtctggccag cagagtgaag tgcccggaag
    10801 catccccaac agctggaagt ggaccgtgga acacatcgtg tacaaggccc tgcggagcca
    10861 cattctccct cctaagcact tcaccgagga cggcaacatc ctgcagctgg ccaacctgcc
    10921 cgacctttat aaggttttct aaccggtcat catcaccatc accattgagt ttaaacccgc
    10981 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
    11041 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgagaaaatt
    11101 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
    11161 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
    11221 tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg
    11281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    11341 atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca
    11401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    11461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    11521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    11581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    11641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    11701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    11761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    11821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    11881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    11941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    12001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    12061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    12121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    12181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    12241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    12301 acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    12361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    12421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    12481 tcagcg
//
