LOCUS       sequence-429357-        9040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing nicking sgRNAs for the induction of 
            EGFR L858R and T790M mutations, through tandem U6 expression of the 
            two sgRNAs using independent U6 promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-EGFR-L858R-T790M-dual-nick sgRNA
FEATURES             Location/Qualifiers
     source          1..9040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(62..81)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     272..293
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(412..432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        417..746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      597..732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     659..678
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        794..841
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             860..1234
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1364..1485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1401..1420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1455..1474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1534..1550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1534..1550)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1547..1569)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1582..1588,1589..1606,1607..1612))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1627..1648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1765..1782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1936..2524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2016..2035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2695..3486,3487..3555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3318..3337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3556..3660)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3735..3754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3930..4309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4311..4509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4464..4484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4527..4707
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4754..4879
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5372..5605
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5790..5834
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6132..6249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6300..6548
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6570..6645
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6714..6962
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6984..7059
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7170..7381
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      7400..7409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7406..7804
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7820..8408
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7873..7893)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8291..8302)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8480..8713
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8739..8756)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8745..8969
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9015..9040,1..403)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
       61 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
      301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
      361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
      421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
      541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
      601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
      661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
      721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
      781 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
      841 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
      901 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
      961 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     1021 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     1081 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     1141 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     1201 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     1261 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     1321 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     1381 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1441 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     1501 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1561 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1621 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1681 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1741 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1801 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2341 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2581 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2641 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2701 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2761 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2821 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2881 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2941 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3001 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3061 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3121 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3181 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3241 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3301 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3361 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3421 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3481 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3541 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3601 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3661 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     3721 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     3781 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     3841 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     3901 gatgtacggg ccagatatac gcgcgcgttg acattgatta ttgactagtt attaatagta
     3961 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     4021 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4081 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     4141 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     4201 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     4261 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     4321 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     4381 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     4441 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     4501 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4561 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4621 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4681 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     4741 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     4801 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     4861 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     4921 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     4981 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     5041 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     5101 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     5161 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     5221 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     5281 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     5341 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     5401 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     5461 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     5521 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     5581 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     5641 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     5701 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     5761 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     5821 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     5881 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     5941 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     6001 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     6061 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     6121 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     6181 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     6241 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat taaggtaccg
     6301 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     6361 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     6421 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     6481 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     6541 gaaacaccgt tactttgcct ccttctgcag ttttagagct agaaatagca agttaaaata
     6601 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct
     6661 agccgggttt attacaggga cagcagagat ccagtttggt taattaaggt accgagggcc
     6721 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
     6781 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     6841 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     6901 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     6961 ccgcctccag gaagcctacg tgagttttag agctagaaat agcaagttaa aataaggcta
     7021 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaatt cgctagctag
     7081 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gctagctagg tcttgaaagg
     7141 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     7201 gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa
     7261 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     7321 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     7381 ggaccggttc tagagcgctg ccaccatggc caagcctttg tctcaagaag aatccaccct
     7441 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     7501 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     7561 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     7621 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     7681 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     7741 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     7801 ctaaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7861 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7921 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7981 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8041 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8101 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8161 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8221 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8281 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8341 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8401 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8461 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     8521 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8581 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8641 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8701 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8761 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8821 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8881 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8941 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     9001 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
//
