LOCUS       sequence-429359-        8502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing epegRNA to induce EGFR L858R mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-EGFR-L858R-tmpknot-epegRNA
FEATURES             Location/Qualifiers
     source          1..8502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(165..176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             354..587
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    665..786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(702..721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     756..775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      826..961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(981..1000)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(982..1000)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1010..1027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1010..1026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1019..1041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1168..1623
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1255..1274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1465..1486
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1649..1753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1754..1822,1823..2614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1972..1991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2785..3373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3274..3293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3527..3544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3661..3682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3697..3702,3703..3720,3721..3727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3735..3751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3740..3762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3759..3775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3759..3775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3794..3814
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3796..3814
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3842..4068
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4069..4249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4296..4421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4914..5147
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5332..5376
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5554..5802
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5554..5574
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5725..5744
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5824..5899
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    6134..6251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6390..7568
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6621..7559
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7516..7536
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7581..8180
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7581..7600)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8077..8097
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(8196..8502,1..282)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8249..8269)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
       61 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
      121 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
      181 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
      241 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
      301 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      361 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
      421 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      481 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      541 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
      601 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
      661 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      721 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      781 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
      841 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      901 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      961 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     1021 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     1081 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1141 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1201 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1261 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1321 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1381 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1441 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1501 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1561 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1621 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1681 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1741 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1801 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1861 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1921 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1981 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     2041 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     2101 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2161 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2221 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2281 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2341 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2401 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2461 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2521 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2581 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2641 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2701 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2761 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2821 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2881 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2941 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3001 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3061 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3121 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3181 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3241 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3301 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3361 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3421 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3481 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3541 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3601 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3661 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3721 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     3781 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     3841 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     3901 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     3961 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     4021 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     4081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     4261 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     4321 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     4381 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4441 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4501 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     4561 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     4621 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     4681 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     4741 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     4801 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     4861 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     4921 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     4981 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     5041 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     5101 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     5161 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     5221 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     5281 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     5341 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     5401 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     5461 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     5521 cagacccacc tcccaacccc gaggggaccc agagagggcc tatttcccat gattccttca
     5581 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
     5641 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     5701 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     5761 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgcaagatc acagattttg
     5821 ggcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     5881 agtgggaccg agtcggtcca gtttggcccg cccaaaatct gtgatcttca ggagcccccc
     5941 ccctgaaccc aggataaccc tcaaagtcgg ggggcttttt ttaagcttgg gccgctcgag
     6001 gtacctctct acatatgaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6061 ggccgcgttg ctggcgttgg atccaagctt ggcgtaacta gatcttgaga caaatggcag
     6121 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     6181 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     6241 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     6301 gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     6361 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     6421 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
     6481 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     6541 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     6601 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     6661 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     6721 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     6781 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     6841 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     6901 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     6961 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     7021 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     7081 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     7141 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     7201 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     7261 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     7321 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     7381 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     7441 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     7501 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     7561 tgtcgtgacg tacggccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7621 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7681 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7741 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg
     7801 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7861 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7921 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc
     7981 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8041 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8101 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8161 cccgcaagcc cggtgcctga acgcgttaag tcgacaatca acctctggat tacaaaattt
     8221 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     8281 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     8341 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8401 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8461 agctcctttc cgggactttc gctttccccc tccctattgc ca
//
