LOCUS       sequence-429362-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing epegRNA to induce EGFR T790M mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-EGFR-T790M-tmpknot-epegRNA
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    240..365
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             412..592
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(593..819)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(847..867)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(847..865)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(886..902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(886..902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(899..921)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    910..926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(934..940,941..958,959..964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    979..1000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1117..1134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1288..1876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1368..1387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2047..2838,2839..2907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2670..2689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2908..3012)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3038..3493)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3175..3196)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3387..3406
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3620..3642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3634..3651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3635..3651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3661..3680
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3661..3679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3700..3835
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3875..3996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3886..3905)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3940..3959
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4074..4307
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4379..4967
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4485..4496
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4894..4914
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4983..5582)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5066..5086)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5563..5582
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5595..6773)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5604..6542
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5627..5647)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6912..7029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(7269..7344)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7366..7614)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7424..7443)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7594..7614)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7792..7836)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8021..8254
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt
       61 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact
      121 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt
      181 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg
      241 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc
      301 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga
      361 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga
      421 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
      481 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
      541 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt
      601 atcgagctag gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa
      661 tccatgtcag acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca
      721 ccaatcggca tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta
      781 ccatgttgca agactacaag agtattgcat aagactacat taagcttgca gctccagctt
      841 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
      901 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      961 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1021 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2341 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2641 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3001 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     3061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3121 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3481 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3541 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3601 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     3661 taatacgact cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc
     3721 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3781 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3841 gagttagggg cgggatagct agagccagac atgataagat acattgatga gtttggacaa
     3901 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3961 ttatttgtaa ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt
     4021 ctttgcaagt tataaatact gaataataag atgacatgaa ctactatacg tactgctaga
     4081 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     4141 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     4201 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     4261 agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc
     4321 ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta aagtcgacgc
     4381 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     4441 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     4501 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     4561 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     4621 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     4681 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     4741 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     4801 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     4861 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     4921 agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc gacttaacgc
     4981 gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     5041 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     5101 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     5161 ctgcccagac ccttgccctg gtggtcgggc gagactccga cggtggccag gaaccacgcg
     5221 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     5281 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     5341 ctctccggcg tggtccagac cgccacggcg gcgccgtcgt ccgcgaccca caccttgccg
     5401 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     5461 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     5521 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     5581 atggtggccg tacgtcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
     5641 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
     5701 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
     5761 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
     5821 tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag
     5881 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
     5941 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
     6001 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
     6061 agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
     6121 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
     6181 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
     6241 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
     6301 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
     6361 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
     6421 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
     6481 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
     6541 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     6601 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     6661 cagtttaccc cgcgccacct tctctaggca ccggatcaat tgccgacccc tccccccaac
     6721 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gccaattccc
     6781 actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt ttaaaacttt
     6841 atccatcttt gcacccgggc cccctcgagc cggcgccaaa gtggatctct gctgtccctg
     6901 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     6961 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     7021 cttttaaaat tgtggatgaa tactgccatt tgtctcaaga tctagttacg ccaagcttgg
     7081 atccaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7141 catgtcatat gtagagaggt acctcgagcg gcccaagctt aaaaaaagcc ccccgacttt
     7201 gagggttatc ctgggttcag ggggggggct cctgacagga ggcagccgaa gggcatgagc
     7261 tgcatgatgg accgactcgg tcccactttt tcaagttgat aacggactag ccttatttta
     7321 acttgctatt tctagctcta aaacttcggc tgcctcctgg actacggtgt ttcgtccttt
     7381 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
     7441 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
     7501 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct
     7561 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctctctggg
     7621 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     7681 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     7741 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     7801 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     7861 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     7921 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     7981 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     8041 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     8101 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     8161 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     8221 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     8281 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     8341 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     8401 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     8461 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatag
//
