LOCUS       sequence-429364-        8577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or 
            G13A mutation using PE3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-KRAS-G121C/G13A-nick sgRNA
FEATURES             Location/Qualifiers
     source          1..8577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..171
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    304..528
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     517..534
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             560..793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    865..1453
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             971..982
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1380..1400
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1469..1867)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      1864..1873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1892..2103)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(2165..2240)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(2262..2510)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2320..2339)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2490..2510)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2561..2678
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2976..3020)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3205..3438
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3931..4056
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4103..4283
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4301..4499)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4326..4346)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4501..4880
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5056..5075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5150..5254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5255..5323,5324..6115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5473..5492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6286..6874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6775..6794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7028..7045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7162..7183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7198..7203,7204..7221,7222..7228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7236..7252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7241..7263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7260..7276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7260..7276
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7325..7446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7336..7355)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7390..7409
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7576..7950)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7969..8016)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(8064..8393)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8078..8213
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8132..8151)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8378..8398
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(join(8407..8577,1..258))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8517..8538)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
       61 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
      121 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
      181 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
      241 cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag
      301 aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct
      361 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
      421 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
      481 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
      541 agcgggttta aacgggccct gctagagatt ttccacactg actaaaaggg tctgagggat
      601 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
      661 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
      721 tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg ttgggagtga
      781 attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg
      841 taagtcattg gtcttaaagt cgacgcgggg aggcggccca aagggagatc cgactcgtct
      901 gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa
      961 ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg
     1021 tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac accacggaat
     1081 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
     1141 ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg
     1201 caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc
     1261 tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga
     1321 aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac
     1381 atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat
     1441 ccagaggttg attgtcgact taacgcgttt agccctccca cacataacca gagggcagca
     1501 attcacgaat cccaactgcc gtcggctgtc catcactgtc cttcactatg gctttgatcc
     1561 caggatgcag atcgagaagc acctgtcggc accgtccgca ggggctcaag atgcccctgt
     1621 tctcatttcc gatcgcgacg atacaagtca ggttgccagc tgccgcagca gcagcagtgc
     1681 ccagcaccac gagttctgca caaggtcccc cagtaaaatg atatacattg acaccagtga
     1741 agatgcggcc gtcgctagag agagctgcgc tggcgacgct gtagtcttca gagatgggga
     1801 tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga gacaaaggct
     1861 tggccatggt ggcagcgctc tagaaccggt cctgtgttct ggcggcaaac ccgttgcgaa
     1921 aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa
     1981 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
     2041 cggatcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
     2101 cccactgacg ggcaccggag ccaattccca ctcctttcaa gacctagcta gcgaattcaa
     2161 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
     2221 gctatttcta gctctaaaac acaatagagg taaatcttgt cggtgtttcg tcctttccac
     2281 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
     2341 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
     2401 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca
     2461 gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc ggtaccttaa
     2521 ttaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
     2581 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     2641 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
     2701 gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg
     2761 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     2821 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     2881 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     2941 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     3001 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     3061 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     3121 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     3181 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     3241 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     3301 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     3361 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     3421 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     3481 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     3541 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     3601 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     3661 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     3721 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     3781 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     3841 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     3901 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     3961 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     4021 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     4081 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     4141 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     4201 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     4261 atctggtcta accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca
     4321 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     4381 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     4441 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     4501 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     4561 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     4621 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     4681 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     4741 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     4801 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     4861 actagtcaat aatcaatgtc aacgcgcgcg tatatctggc ccgtacatcg cgaagcagcg
     4921 caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg
     4981 tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat
     5041 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg
     5101 gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5161 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5221 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5281 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5341 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5401 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5461 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5521 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5581 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5641 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5701 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5761 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5821 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5881 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5941 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6001 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6061 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6121 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6181 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6241 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6301 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6361 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6421 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6481 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6541 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6601 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6661 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6721 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6781 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6841 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6901 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6961 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7021 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7081 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     7141 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7201 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7261 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtatacagac
     7321 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     7381 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     7441 caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     7501 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     7561 atctcgtagc acgtgtcagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg
     7621 gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc
     7681 ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag
     7741 gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct
     7801 gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg
     7861 ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag
     7921 caccggaacg gcactggtca acttggccat ggtttagttc ctcaccttgt cgtattatac
     7981 tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcaga tccgaaaatg
     8041 gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     8101 tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt
     8161 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     8221 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     8281 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     8341 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     8401 agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     8461 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     8521 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaa
//
