LOCUS       sequence-429365-       13031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor with P2A-human 
            P53DD and IRES-human MLH1dn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 13031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: pCMV-PE-Plus
FEATURES             Location/Qualifiers
     source          1..13031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(237..1028,1029..1097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     860..879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1098..1202)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1281..1300)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1472..1851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1852..2055
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2006..2026
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2097..2116
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2097..2115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2165..2185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2186..6286
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6344..6364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8465..8485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8495..8521
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8531..8587
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    join(8912..9486,9487..9489,9490..9498)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9079..9096)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9306..9325
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11766..11783
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11806..11823)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11812..12036
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12107..12123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12107..12123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12120..12142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12131..12147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12155..12161,12162..12179,12180..12185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12200..12221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12338..12355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12509..13031,1..66))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12589..12608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
       61 tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca
      121 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1201 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat
     1261 cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     1321 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     1381 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     1441 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     1501 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     1561 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     1621 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     1681 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1741 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     1801 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     1861 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     1921 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     1981 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     2041 tatataagca gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgctaat
     2101 acgactcact atagggagag ccgccaccat gaaacggaca gccgacggaa gcgagttcga
     2161 gtcaccaaag aagaagcgga aagtcgacaa gaagtacagc atcggcctgg acatcggcac
     2221 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     2281 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     2341 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     2401 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     2461 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     2521 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     2581 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     2641 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     2701 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     2761 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     2821 tgccagactg agcaagagca gaaagctgga aaatctgatc gcccagctgc ccggcgagaa
     2881 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     2941 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     3001 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     3061 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     3121 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     3181 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     3241 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     3301 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     3361 gaagagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     3421 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     3481 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     3541 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     3601 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     3661 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     3721 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     3781 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     3841 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     3901 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     3961 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     4021 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     4081 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     4141 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     4201 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     4261 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     4321 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     4381 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     4441 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     4501 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     4561 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     4621 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     4681 gtccgactac gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga
     4741 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     4801 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     4861 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     4921 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     4981 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     5041 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     5101 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     5161 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     5221 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     5281 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     5341 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     5401 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     5461 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     5521 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     5581 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     5641 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     5701 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     5761 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     5821 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     5881 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     5941 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     6001 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     6061 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     6121 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     6181 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     6241 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactccg gcggaagctc
     6301 tggtggcagc aagcggaccg ccgacggctc tgaattcgag agccctaaga agaaaagaaa
     6361 ggtgagcgga ggctctagcg gcggaagcac cctgaacatt gaagacgagt atagactgca
     6421 tgaaacaagc aaggaacccg acgtgtccct gggctccacc tggctgtccg actttcccca
     6481 ggcctgggcc gagacaggag gaatgggcct ggccgtgcgg caggcacccc tgatcatccc
     6541 tctgaaggcc acctctacac ccgtgagcat caagcagtac cctatgtctc aggaggccag
     6601 actgggcatc aagcctcaca tccagaggct gctggaccag ggcatcctgg tgccatgcca
     6661 gagcccctgg aacacaccac tgctgcccgt gaagaagcca ggcaccaatg actatagacc
     6721 cgtgcaggat ctgagagagg tgaacaagag ggtggaggat atccacccca ccgtgcccaa
     6781 cccttacaat ctgctgtccg gcctgccccc ttctcaccag tggtatacag tgctggacct
     6841 gaaggatgcc ttcttttgtc tgagactgca ccctaccagc cagccactgt tcgcctttga
     6901 gtggagggac cctgagatgg gcatctctgg ccagctgacc tggacacgcc tgcctcaggg
     6961 cttcaagaat agcccaacac tgtttaacga ggccctgcac cgcgacctgg cagatttccg
     7021 gatccagcac ccagatctga tcctgctgca gtacgtggac gatctgctgc tggccgccac
     7081 cagcgagctg gattgccagc agggaacacg cgccctgctg cagaccctgg gaaacctggg
     7141 atatagggca tccgccaaga aggcccagat ctgtcagaag caggtgaagt acctgggcta
     7201 tctgctgaag gagggccaga gatggctgac agaggccagg aaggagacag tgatgggcca
     7261 gccaacaccc aagaccccaa gacagctgag ggagttcctg ggcaaagcag gattttgcag
     7321 gctgttcatc ccaggattcg cagagatggc agcacctctg tacccactga ccaagccggg
     7381 caccctgttt aattggggcc ctgaccagca gaaggcctat caggagatca agcaggccct
     7441 gctgacagca ccagccctgg gcctgccaga cctgaccaag cctttcgagc tgtttgtgga
     7501 tgagaagcag ggctacgcca agggcgtgct gacccagaag ctgggaccat ggagacggcc
     7561 cgtggcctat ctgtccaaga agctggaccc agtggcagca ggatggccac catgcctgag
     7621 gatggtggca gcaatcgccg tgctgacaaa ggatgccggc aagctgacca tgggacagcc
     7681 actggtcatc ctggcaccac acgcagtgga ggccctggtg aagcagcctc cagatcgctg
     7741 gctgtctaac gcccggatga cacactacca ggccctgctg ctggacaccg atcgcgtgca
     7801 gtttggccct gtggtggccc tgaatccagc caccctgctg cctctgccag aggagggcct
     7861 gcagcacaac tgtctggaca tcctggcaga ggcacacgga acaaggccag acctgaccga
     7921 tcagcccctg cctgacgccg atcacacatg gtataccgat ggaagctccc tgctgcagga
     7981 gggccagagg aaggcaggag cagcagtgac cacagagaca gaagtgatct gggccaaggc
     8041 cctgccagca ggcacatccg cccagcgggc cgagctgatc gccctgaccc aggccctgaa
     8101 gatggccgag ggcaagaagc tgaacgtgta cacagactcc agatatgcct tcgccaccgc
     8161 acacatccac ggagagatct acaggcgccg gggctggctg acctctgagg gcaaggagat
     8221 caagaacaag gatgagatcc tggccctgct gaaggccctg tttctgccca agcggctgag
     8281 catcatccac tgtcctggac accagaaggg acactccgcc gaggcaaggg gcaatcggat
     8341 ggccgaccag gccgccagaa aggctgctat tactgaaact cccgacactt ccactctgct
     8401 gattgaaaac tcctcccctt ctggcggctc aaaaagaacc gccgacggca gcgaattcga
     8461 gtctcccaag aagaagagga aagtcggctc tggccctgcc gctaagagag tgaagctgga
     8521 cggatccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     8581 tggaccgatg gagcctcacc acgagctgcc cccagggagc actaagcgag cactgcccaa
     8641 caacaccagc tcctctcccc agccaaagaa gaaaccactg gatggagaat atttcaccct
     8701 tcagatccgt gggcgtgagc gcttcgagat gttccgagag ctgaatgagg ccttggaact
     8761 caaggatgcc caggctggga aggagccagg ggggagcagg gctcactcca gccacctgaa
     8821 gtccaaaaag ggtcagtcta cctcccgcca taaaaaactc atgttcaaga cagaagggcc
     8881 tgactcagac tgaccggtgt ttaggcgcgc ccccctctcc ctcccccccc cctaacgtta
     8941 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     9001 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     9061 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     9121 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     9181 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     9241 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     9301 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     9361 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     9421 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     9481 gataatatgg ccacaaccat gagcttcgtt gctggagtca tccggagact ggacgagaca
     9541 gtggtgaaca gaattgccgc cggcgaggtg atccagagac ctgccaatgc aataaaggag
     9601 atgatcgaga actgtctgga cgccaagtcc acaagcattc aggtgatcgt gaaggagggc
     9661 ggactgaagc tgatccagat ccaagacaac ggcacaggca tcagaaagga agatctggac
     9721 atcgtgtgtg aacggttcac cacatctaag ctgcagtctt ttgaggatct ggcctctatc
     9781 agtacctacg gcttcagagg cgaggccctg gccagcatca gccacgtggc ccatgtgacc
     9841 atcaccacca aaaccgccga cggcaaatgc gcttatcgcg ctagctacag cgacggcaag
     9901 ctgaaagccc cgccaaagcc ttgcgccggc aaccagggta cacagataac agtggaggat
     9961 ctgttctaca acatcgccac ccggagaaag gccctgaaaa atcccagcga ggagtacggc
    10021 aagatcctgg aagtcgtcgg cagatactcc gtgcacaacg ccggaatcag ctttagcgta
    10081 aagaagcagg gagaaaccgt ggccgatgtg cgcaccctgc caaatgccag caccgtggat
    10141 aacatcagaa gcattttcgg aaatgccgtg tccagagaac tgatcgagat cggctgcgaa
    10201 gataagaccc tggcttttaa gatgaacggc tacatcagca acgccaatta ctctgtgaag
    10261 aagtgcatct ttcttctgtt catcaaccac agactggtgg aaagcaccag cctgcggaaa
    10321 gccatcgaga cagtgtacgc cgcctacctg cctaagaaca cccacccctt cctgtacctg
    10381 agcctcgaga tcagccctca gaacgtggac gtcaatgtgc atcctacaaa gcacgaggtg
    10441 cacttcctgc acgaggaaag catcctggaa agagtgcagc agcacattga gagcaagctg
    10501 ctgggctcta acagcagcag aatgtacttc acacagaccc tgctgcctgg cctggccggc
    10561 ccctcaggcg aaatggttaa gtccacaacc tctctgacct catccagcac cagcggttct
    10621 tccgataagg tgtacgccca ccagatggtg cggaccgact ctcgggagca gaagctggac
    10681 gcctttctgc aacctctgag caaacctctg agctctcagc ctcaggccat cgtgaccgag
    10741 gacaagacag atatctcctc cggccgtgcc agacagcagg acgaagaaat gctcgagctg
    10801 ccagctcctg ccgaggtggc cgccaagaac cagagcctgg agggagatac cacaaagggc
    10861 accagcgaaa tgagcgagaa gcggggccct acctccagca accccagaaa acggcaccgg
    10921 gaggacagcg acgtggaaat ggtggaggac gacagcagaa aggaaatgac agccgcttgt
    10981 acccctagaa gaagaatcat caacctgacc tccgtgctga gcctgcagga ggagatcaac
    11041 gagcagggcc acgaggtgct gagagagatg ctgcacaatc acagcttcgt gggctgcgtg
    11101 aaccctcaat gggccctggc tcagcatcaa acaaagctgt acctgctgaa caccaccaag
    11161 ctgagcgaag agctgttcta ccagatcctc atctacgact tcgccaactt cggcgtgcta
    11221 cgcctgagcg agcccgcccc tctgtttgac ctggccatgc tggctctgga tagcccagaa
    11281 agcggctgga cagaagagga cggacctaaa gaggggctgg ctgaatacat cgtggagttc
    11341 ctgaagaaaa aggccgagat gctggccgac tacttttctc tggaaatcga cgaggaaggc
    11401 aacctgatcg gcctgcctct gctgatcgat aactacgtgc ctcccctgga aggcctgccc
    11461 atcttcatcc tgagactggc tacagaggtg aactgggacg aggaaaagga atgcttcgag
    11521 tctctgagca aggagtgcgc catgttctat agcatcagaa aacagtacat ctctgaagag
    11581 agcactctgt ctggccagca gagtgaagtg cccggaagca tccccaacag ctggaagtgg
    11641 accgtggaac acatcgtgta caaggccctg cggagccaca ttctccctcc taagcacttc
    11701 accgaggacg gcaacatcct gcagctggcc aacctgcccg acctttataa ggttttctaa
    11761 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
    11821 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
    11881 ccactcccac tgtcctttcc taataaaatg agaaaattgc atcgcattgt ctgagtaggt
    11941 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
    12001 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
    12061 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    12121 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    12181 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12241 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12301 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    13021 gcaaacaaac c
//
