LOCUS       sequence-429366-       10183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: pCMV-PEmax-P2A-hP53DD
FEATURES             Location/Qualifiers
     source          1..10183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        48..427
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        428..631
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     582..602
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     673..692
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        673..691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             741..761
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             762..4862
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4920..4940
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7041..7061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7071..7097
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7107..7163
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7494..7511
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7534..7551)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7540..7764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7835..7851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7835..7851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7848..7870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7859..7875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7883..7889,7890..7907,7908..7913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7928..7949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8066..8083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8237..8825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8317..8336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8996..9787,9788..9856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9619..9638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9857..9961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10040..10059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
       61 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      121 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      181 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      241 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      301 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      361 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      421 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      481 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      541 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      601 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagag
      661 atccgcggcc gctaatacga ctcactatag ggagagccgc caccatgaaa cggacagccg
      721 acggaagcga gttcgagtca ccaaagaaga agcggaaagt cgacaagaag tacagcatcg
      781 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
      841 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
      901 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
      961 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     1021 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     1081 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     1141 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     1201 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     1261 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     1321 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     1381 ccaaggccat cctgtctgcc agactgagca agagcagaaa gctggaaaat ctgatcgccc
     1441 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     1501 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     1561 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     1621 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     1681 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     1741 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     1801 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     1861 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     1921 aactgctcgt gaagctgaag agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     1981 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     2041 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     2101 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     2161 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     2221 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     2281 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     2341 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     2401 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     2461 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     2521 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     2581 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     2641 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     2701 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     2761 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     2821 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     2881 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     2941 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     3001 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     3061 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     3121 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     3181 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     3241 tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag agctttctga
     3301 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     3361 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     3421 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     3481 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     3541 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     3601 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     3661 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     3721 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     3781 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     3841 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     3901 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     3961 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     4021 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     4081 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     4141 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     4201 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     4261 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     4321 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     4381 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     4441 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     4501 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     4561 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     4621 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     4681 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     4741 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     4801 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggtg
     4861 actccggcgg aagctctggt ggcagcaagc ggaccgccga cggctctgaa ttcgagagcc
     4921 ctaagaagaa aagaaaggtg agcggaggct ctagcggcgg aagcaccctg aacattgaag
     4981 acgagtatag actgcatgaa acaagcaagg aacccgacgt gtccctgggc tccacctggc
     5041 tgtccgactt tccccaggcc tgggccgaga caggaggaat gggcctggcc gtgcggcagg
     5101 cacccctgat catccctctg aaggccacct ctacacccgt gagcatcaag cagtacccta
     5161 tgtctcagga ggccagactg ggcatcaagc ctcacatcca gaggctgctg gaccagggca
     5221 tcctggtgcc atgccagagc ccctggaaca caccactgct gcccgtgaag aagccaggca
     5281 ccaatgacta tagacccgtg caggatctga gagaggtgaa caagagggtg gaggatatcc
     5341 accccaccgt gcccaaccct tacaatctgc tgtccggcct gcccccttct caccagtggt
     5401 atacagtgct ggacctgaag gatgccttct tttgtctgag actgcaccct accagccagc
     5461 cactgttcgc ctttgagtgg agggaccctg agatgggcat ctctggccag ctgacctgga
     5521 cacgcctgcc tcagggcttc aagaatagcc caacactgtt taacgaggcc ctgcaccgcg
     5581 acctggcaga tttccggatc cagcacccag atctgatcct gctgcagtac gtggacgatc
     5641 tgctgctggc cgccaccagc gagctggatt gccagcaggg aacacgcgcc ctgctgcaga
     5701 ccctgggaaa cctgggatat agggcatccg ccaagaaggc ccagatctgt cagaagcagg
     5761 tgaagtacct gggctatctg ctgaaggagg gccagagatg gctgacagag gccaggaagg
     5821 agacagtgat gggccagcca acacccaaga ccccaagaca gctgagggag ttcctgggca
     5881 aagcaggatt ttgcaggctg ttcatcccag gattcgcaga gatggcagca cctctgtacc
     5941 cactgaccaa gccgggcacc ctgtttaatt ggggccctga ccagcagaag gcctatcagg
     6001 agatcaagca ggccctgctg acagcaccag ccctgggcct gccagacctg accaagcctt
     6061 tcgagctgtt tgtggatgag aagcagggct acgccaaggg cgtgctgacc cagaagctgg
     6121 gaccatggag acggcccgtg gcctatctgt ccaagaagct ggacccagtg gcagcaggat
     6181 ggccaccatg cctgaggatg gtggcagcaa tcgccgtgct gacaaaggat gccggcaagc
     6241 tgaccatggg acagccactg gtcatcctgg caccacacgc agtggaggcc ctggtgaagc
     6301 agcctccaga tcgctggctg tctaacgccc ggatgacaca ctaccaggcc ctgctgctgg
     6361 acaccgatcg cgtgcagttt ggccctgtgg tggccctgaa tccagccacc ctgctgcctc
     6421 tgccagagga gggcctgcag cacaactgtc tggacatcct ggcagaggca cacggaacaa
     6481 ggccagacct gaccgatcag cccctgcctg acgccgatca cacatggtat accgatggaa
     6541 gctccctgct gcaggagggc cagaggaagg caggagcagc agtgaccaca gagacagaag
     6601 tgatctgggc caaggccctg ccagcaggca catccgccca gcgggccgag ctgatcgccc
     6661 tgacccaggc cctgaagatg gccgagggca agaagctgaa cgtgtacaca gactccagat
     6721 atgccttcgc caccgcacac atccacggag agatctacag gcgccggggc tggctgacct
     6781 ctgagggcaa ggagatcaag aacaaggatg agatcctggc cctgctgaag gccctgtttc
     6841 tgcccaagcg gctgagcatc atccactgtc ctggacacca gaagggacac tccgccgagg
     6901 caaggggcaa tcggatggcc gaccaggccg ccagaaaggc tgctattact gaaactcccg
     6961 acacttccac tctgctgatt gaaaactcct ccccttctgg cggctcaaaa agaaccgccg
     7021 acggcagcga attcgagtct cccaagaaga agaggaaagt cggctctggc cctgccgcta
     7081 agagagtgaa gctggacgga tccggcgcaa caaacttctc tctgctgaaa caagccggag
     7141 atgtcgaaga gaatcctgga ccgatggagc ctcaccacga gctgccccca gggagcacta
     7201 agcgagcact gcccaacaac accagctcct ctccccagcc aaagaagaaa ccactggatg
     7261 gagaatattt cacccttcag atccgtgggc gtgagcgctt cgagatgttc cgagagctga
     7321 atgaggcctt ggaactcaag gatgcccagg ctgggaagga gccagggggg agcagggctc
     7381 actccagcca cctgaagtcc aaaaagggtc agtctacctc ccgccataaa aaactcatgt
     7441 tcaagacaga agggcctgac tcagactgac cggtgtttag gcgcgccacc ggtcatcatc
     7501 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7561 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7621 tcctttccta ataaaatgag aaaattgcat cgcattgtct gagtaggtgt cattctattc
     7681 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7741 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     7801 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7861 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     7921 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7981 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8041 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8641 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac
     8881 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8941 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9001 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9061 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9121 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9181 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9241 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9301 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9361 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9421 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9481 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9541 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9601 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9661 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9721 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9781 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9841 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9901 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9961 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
    10021 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    10081 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    10141 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggt
//
