LOCUS       sequence-429391-        5040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0390m_3xC2 is an optimized enhancer sequence designed to drive 
            AAV-mediated Cre recombination in brain astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14801 -
            pAAV-AiE0390m_3xC2-minBG-iCre(R297T...
FEATURES             Location/Qualifiers
     source          1..5040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             988..1008
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2074..2185
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2303..2443
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2303..2432
                     /label=AAV2 ITR
     rep_origin      2518..2973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2605..2624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2815..2836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2990..3009)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3109..3131
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3169..3187)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3255..3359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3360..3428,3429..4220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3578..3597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4391..4979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4880..4899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgaaagg aaagagcttg ctttcaacct caaaagctag
      181 gaggaaaggg ctctgaaatt tgctcagaat tcccaattca ccattagcct gtttcttcct
      241 ttagcctcaa ggcattctcc gctttttgaa aagatgttaa gaaattcagt cacaatagag
      301 agcctagttt tgaacatgtt tcactcggtc cattgaggtc tgggctccag cctttgtgtg
      361 gggtgaattg agctgagcgg ctagctggtt ggaggaaagg aaagagcttg ctttcaacct
      421 caaaagctag gaggaaaggg ctctgaaatt tgctcagaat tcccaattca ccattagcct
      481 gtttcttcct ttagcctcaa ggcattctcc gctttttgaa aagatgttaa gaaattcagt
      541 cacaatagag agcctagttt tgaacatgtt tcactcggtc cattgaggtc tgggctccag
      601 cctttgtgtg gggtgaattg agctgagcgg ctagctggtt ggaggaaagg aaagagcttg
      661 ctttcaacct caaaagctag gaggaaaggg ctctgaaatt tgctcagaat tcccaattca
      721 ccattagcct gtttcttcct ttagcctcaa ggcattctcc gctttttgaa aagatgttaa
      781 gaaattcagt cacaatagag agcctagttt tgaacatgtt tcactcggtc cattgaggtc
      841 tgggctccag cctttgtgtg gggtgaattg agctgagcgg ctagctggtt ggaggagctc
      901 gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca
      961 gatctttcga agctagccac catggtgccc aagaagaaga ggaaagtctc caacctgctg
     1021 actgtgcacc aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag
     1081 aacctgatgg acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc
     1141 ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct
     1201 gctgaacctg aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg
     1261 aagaccatcc aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct
     1321 cgcccttctg actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg
     1381 gatgctgggg agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc
     1441 agatccctga tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc
     1501 attgcctaca acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc
     1561 tcccgcaccg atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc
     1621 acagctggtg tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc
     1681 tctgtgtctg gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat
     1741 ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt
     1801 gaggccaccc accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc
     1861 tggtctggcc actctgccag agtgggtgct gccaccgaca tggccagggc tggtgtgtcc
     1921 atccctgaaa tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc
     1981 agaaacctgg actctgagac tggggccatg gtgaggctgc tcgaagatgg ggactgaggc
     2041 gcgccgaatt caagcttctc gagagatctt cgactgtgcc ttctagttgc cagccatctg
     2101 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     2161 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     2221 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgagatct
     2281 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     2341 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     2401 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     2461 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     2521 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2581 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2641 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2701 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     2761 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2821 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     2881 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2941 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     3001 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3061 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3121 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3181 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
