LOCUS       sequence-429470-        1278 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DV1 E dimer with fusion loop mutation and increased expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Phan TTN, Hvasta MG, Kudlacek ST, Thiono DJ, Tripathy A, Nicely NI, 
            de Silva AM, Kuhlman B
  TITLE     A conserved set of mutations for stabilizing soluble envelope 
            protein dimers from dengue and Zika viruses to advance the 
            development of subunit vaccines.
  JOURNAL   J Biol Chem. 2022 Jul;298(7):102079. doi: 10.1016/j.jbc.2022.102079.
            Epub 2022 May 26.
  PUBMED    35643320
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-07"; volume: "298(7):102079. doi"; pages: " 
            10.1016/j.jbc.2022.102079. Epub 2022 May 26"
COMMENT     Sequence Label: pD1sE_SC12
FEATURES             Location/Qualifiers
     source          1..1278
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1255..1278
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgaagtggg taacctttat ttcccttctt tttctcttta gctcggctta ttccatgaga
       61 tgtgtgggta taggtaaccg agatttcgtt gagggactta gcggagcgac atgggtggac
      121 gtagtgctgg aacacgggaa gtgtgttact gtgatgatga aagacaagcc tacattggac
      181 atagaacttc tcaagacgga agtcacaaat ccagctgtac tgagaaagct ttgcattgag
      241 gccaagatct ctaacacgac tacggattcc cggtgcccta cgcagggaga ggcgacactc
      301 gttgaagagc aggatacgaa ttttgtatgt agaaggacgt tcgtggatcg gggatgggga
      361 aacggctgcg acctgtttgg caaggggagt ctgatcactt gtgcgaaatt taagtgcgtt
      421 acaaaacttg agggaaagat cgtgcaatat gaaaatctta aatactctgt gattgtaaca
      481 gtacacacag gtgatcaaca tcaggtcgga aatgagacga cagagcatgg caccactgcg
      541 acaataacac cacaggcccc tacttcagag attcagctca ccgattatgg agcgcttaca
      601 ttggactgtt cccctcggac cggattggac ttcaatgaaa tggtacttct gactatggag
      661 aagaaatcct ggttggtgca caaacagtgg ttcctggacc tgcctctccc ctggacatca
      721 ggcgcgagta caagccaaga aacatggaat cgccaagacc tcctggtaac ttttaaaacc
      781 gctcacgcca agaaacagga agtagttgtt cttggctccc aggaggggtg gatgcaccgg
      841 gcattgacgg gagcaacgga gatacagact tcaggaacaa caaccatatg gcccgggcat
      901 cttaaatgtc ggctgaaaat ggataagttg acgcttaaag ggatgagtta cgttatgtgt
      961 accggctcat tcaaattgga aaaagaggtc gcagagacac agcacggtac tgtgctcgta
     1021 caggtcaaat atgaaggtac tgatgcgcct tgcaagatac catttagtag ccaagatgaa
     1081 aagggggtta ctcaaaacgg gcggctcatt accgccaacc ccattgtaac agacaaggaa
     1141 aaaccagtaa atatagaggc agaacctcct tttggtgaat catatatagt agtaggggct
     1201 ggagagaaag ctctgaagct gtcatggttt aagaaaggca gcagcggcgg cagccatcac
     1261 caccatcatc accatcat
//
