LOCUS       sequence-429471-        1278 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DV2 stable E dimer with fusion loop mutation and increased 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Phan TTN, Hvasta MG, Kudlacek ST, Thiono DJ, Tripathy A, Nicely NI, 
            de Silva AM, Kuhlman B
  TITLE     A conserved set of mutations for stabilizing soluble envelope 
            protein dimers from dengue and Zika viruses to advance the 
            development of subunit vaccines.
  JOURNAL   J Biol Chem. 2022 Jul;298(7):102079. doi: 10.1016/j.jbc.2022.102079.
            Epub 2022 May 26.
  PUBMED    35643320
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-07"; volume: "298(7):102079. doi"; pages: " 
            10.1016/j.jbc.2022.102079. Epub 2022 May 26"
COMMENT     Sequence Label: pD2sE_SC12
FEATURES             Location/Qualifiers
     source          1..1278
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1255..1278
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgaagtggg taacctttat ttcccttctt tttctcttta gctcggctta ttccatgagg
       61 tgtatcggca tgtccaacag ggactttgtg gagggagtga gcggcggcag ctgggtggac
      121 attgtgctgg agcatggaaa gtgcgtgacc gtgatgatga aaaacaagcc caccctggac
      181 ttcgagctca tcaagacaga ggctaaacag cccgccaccc tcaggaagta ctgcatcgag
      241 gccaagctga ccaacacaac aaccgagtcc agatgcccta cacagggcga acccagcctc
      301 aacgaagagc aggacaagag gttcgtgtgc aaacacagca tggtggacag gggctggggc
      361 aatggatgcg acctcttcgg aaaaggcggc atcgtgacct gcgccatgtt caggtgtaaa
      421 aagaacatgg aaggcaaggt ggtgcagccc gaaaatctgg agtataccat cgtgattacc
      481 ccccacagcg gagaggagca cgccgtgggc aatgacaccg gcaagcacgg caaagagatt
      541 aagatcaccc cccagtcctc cattaccgaa gctgaactga caggctacgg caccgtgaca
      601 atggagtgta gccccaggac cggactggat ttcaacgaga tggtcctgct acagatggag
      661 aacaaggcct ggctcgtgca caggcaatgg tttctggatc tgcctctgcc ttggctgcct
      721 ggcgccgata cacagggctc caactggata cagaaagaga ccctcgtcac cttcaagaat
      781 ccccatgcta agaagcagga cgtggtggtg ctgggcagcc aagaaggctg gatgcaccgg
      841 gccctgaccg gagctaccga gatccagatg agctccggca acctgctgtg gcccggccat
      901 ctgaaatgta ggctgaggat ggataagctg caactcaaag gcatgtccta ctccatgtgc
      961 accggaaagt tcaaggtggt gaaagaaatc gccgaaacac agcacggcac catcgtgatc
     1021 agggtgcagt atgagggcga cggctccccc tgtaagatcc ccttcgaaat catggacctg
     1081 gaaaagaggc acgtgctggg caggctcatc accgtgaacc ccattgtcac agagaaagac
     1141 tcccccgtga acatcgaggc cgagcctccc tttggcgact cctacatcat cattggcgtg
     1201 gagcccggac agctcaagct gaactggttc aagaagggca gcagcggcgg cagccatcac
     1261 caccatcatc accatcat
//
