LOCUS       sequence-429472-        1272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DV3 E dimer with fusion loop mutation and increased expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Phan TTN, Hvasta MG, Kudlacek ST, Thiono DJ, Tripathy A, Nicely NI, 
            de Silva AM, Kuhlman B
  TITLE     A conserved set of mutations for stabilizing soluble envelope 
            protein dimers from dengue and Zika viruses to advance the 
            development of subunit vaccines.
  JOURNAL   J Biol Chem. 2022 Jul;298(7):102079. doi: 10.1016/j.jbc.2022.102079.
            Epub 2022 May 26.
  PUBMED    35643320
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-07"; volume: "298(7):102079. doi"; pages: " 
            10.1016/j.jbc.2022.102079. Epub 2022 May 26"
COMMENT     Sequence Label: pD3sE_SC12
FEATURES             Location/Qualifiers
     source          1..1272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1249..1272
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgaagtggg taacctttat ttcccttctt tttctcttta gctcggctta ttccatgcga
       61 tgtgttgggg tgggtaacag ggactttgtc gaaggcctta gcggggcgac atgggtggat
      121 gtggtgcttg aacacggaaa gtgcgtaacg gtgatgatga agaataagcc cacgttggac
      181 atagagctgc agaagactga agccactcaa ctcgctacac tccgaaagct ttgtatagaa
      241 ggtaagatca ctaatattac cactgattct cgctgcccca cccaggggga agctattctt
      301 cctgaggaac aggatcagaa ctatgtctgc aagcacacct acgtggatcg gggatggggt
      361 aacggatgtg acctttttgg caagggctcc ttggtcacat gcgccaagtt tcaatgcctt
      421 gaatccatcg agggaaaggt cgtgcaacac gagaacctga aatacaccgt aattattact
      481 gtacatactg gcgaccaaca tcaggtaggt aatgaaacac agggagtgac agccgaaatt
      541 actccgcagg cgtccaccgt ggaagcaata ctccccgaat acggtactct tggtcttgaa
      601 tgttcccccc gaacaggcct ggatttcaac gaaatgattc tgctgacgat gaaaaataag
      661 gcatggatgg tgcatcgcca atggttcttt gacctccccc tgccgtggac gtccggagcg
      721 accactgaaa cacccacatg gaataggaag gagcttctgg ttacgtttaa aaacgctcat
      781 gctaaaaagc aagaggttgt agtcctcggt agccaggaag gttggatgca ccgggcgctg
      841 accggcgcga cagagatcca gaactccgga ggaactagta tttggcccgg acatctcaaa
      901 tgtagactca agatggataa gctggagttg aagggtatgt cctatgccat gtgtctgaat
      961 acattcgtgc tgaagaaaga ggtatccgag acgcaacatg gaactatctt gattaaggtt
     1021 gaatacaaag gggaggatgc tccatgcaaa ataccttttt ctacagagga cggacaggga
     1081 aaggcacata acggtcgcct gataactgct aacccggtcg tcacgaagaa agaggaacca
     1141 gtgaatatag aagccgagcc acccttcgga gagtccaaca tagtgatcgg cattggtgac
     1201 aaagcactga aaatcaactg gtacaaaaag ggcagcagcg gcggcagcca tcaccaccat
     1261 catcaccatc at
//
