LOCUS       sequence-429473-        1278 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DV4 stable E dimer with fusion loop mutation and increased 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Phan TTN, Hvasta MG, Kudlacek ST, Thiono DJ, Tripathy A, Nicely NI, 
            de Silva AM, Kuhlman B
  TITLE     A conserved set of mutations for stabilizing soluble envelope 
            protein dimers from dengue and Zika viruses to advance the 
            development of subunit vaccines.
  JOURNAL   J Biol Chem. 2022 Jul;298(7):102079. doi: 10.1016/j.jbc.2022.102079.
            Epub 2022 May 26.
  PUBMED    35643320
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-07"; volume: "298(7):102079. doi"; pages: " 
            10.1016/j.jbc.2022.102079. Epub 2022 May 26"
COMMENT     Sequence Label: pD4sE_SC12
FEATURES             Location/Qualifiers
     source          1..1278
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(169..180)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1255..1278
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgaagtggg taacctttat ttcccttctt tttctcttta gctcggctta ttccatgcgg
       61 tgcgtcggcg tcggtaatag agattttgta gagggggtat caggaggtgc ttgggtggat
      121 ctggtgcttg agcatggcaa gtgcgtaacg gtgatgatgc aaggtaaacc taccctcgat
      181 ttcgagttga ctaagacgac agcaaaggaa gtagcgcttc tcaggaccta ttgtatagaa
      241 gcgtcaataa gcaacataac cacagcgacg cggtgcccaa ctcagggtga accctacttg
      301 aaagaggagc aggaccaaca atatatctgt agacgagacg tcgtagatag ggggtgggga
      361 aatggctgtg acctgtttgg caagggcgga gtcgtcacat gcgccaaatt ctcatgctct
      421 ggtaagatca ccggtaatct ggtgcaaata gaaaatcttg aatatacagt cgtggtgaca
      481 gttcacaatg gggacactca cgccgtcggc aacgatacta gtaaccatgg cgttacagct
      541 atgataactc cccgatctcc atcagtggaa gtgaagttgc cagattatgg agagcttacc
      601 ctcgattgtg aaccaagatc cggtattgac ttcaatgaaa tgatattgat gaaaatgaaa
      661 aagaaaacgt ggctggtgca caagcagtgg ttcttggatc tccccttgcc ctggactgca
      721 ggcgcggaca cgtccgaggt tcattggaac tataaagaaa gaatggttac cttcaaagtc
      781 ccgcacgcaa agagacaaga cgtcactgtc ttgggtagtc aggaaggatg gatgcaccgg
      841 gcactggctg gtgctactga agttgactct ggagatggaa accatatgtg gcccgggcat
      901 ctgaagtgca aggttcgcat ggagaagttg agaatcaagg gtatgtcata tacgatgtgt
      961 agtggaaaat ttagcataga taaagagatg gctgaaacac aacatggaac gacagtcgtt
     1021 aaggttaagt atgagggagc tggggctccc tgcaaggttc ccatagaaat ccgggacgtt
     1081 aataaagaga aagtcgtcgg gcgcatcatt agttccactc cattggctga gaacacaaat
     1141 tctgtgacaa acattgagct ggagccgccg ttcggggact catatatcgt cataggagtg
     1201 ggtaattccg cgctgacttt gcactggttc cgcaaaggca gcagcggcgg cagccatcac
     1261 caccatcatc accatcat
//
