LOCUS       sequence-429474-        1308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zika virus stable E dimer with fusion loop mutation and increased 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Phan TTN, Hvasta MG, Kudlacek ST, Thiono DJ, Tripathy A, Nicely NI, 
            de Silva AM, Kuhlman B
  TITLE     A conserved set of mutations for stabilizing soluble envelope 
            protein dimers from dengue and Zika viruses to advance the 
            development of subunit vaccines.
  JOURNAL   J Biol Chem. 2022 Jul;298(7):102079. doi: 10.1016/j.jbc.2022.102079.
            Epub 2022 May 26.
  PUBMED    35643320
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-07"; volume: "298(7):102079. doi"; pages: " 
            10.1016/j.jbc.2022.102079. Epub 2022 May 26"
COMMENT     Sequence Label: pZVsE_SC12
FEATURES             Location/Qualifiers
     source          1..1308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1285..1308
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgaagtggg taacctttat ttcccttctt tttctcttta gctcggctta ttccatccgc
       61 tgcattggtg tgtcaaatcg ggatttcgta gagggaatgt ctgggggcac atgggttgac
      121 attgtcctcg aacatggcaa gtgcgtcaca gtgatgatgc aggataaacc gacggtagac
      181 atcgagcttg ttactaccac tgtgtccaat atggcggaag tgagaagcta ttgttatgag
      241 gcgagcatca gtgatatggc gagtgattca aggtgtccca cccaaggcga ggcgtacttg
      301 gataaacaga gtgatacaca atacgtgtgt aagcgaacac tcgttgacag gggttggggg
      361 aacgggtgcg acctcttcgg taagggaagt ttggtgacat gtgcgaagtt tgcctgctct
      421 aagaaaatga ctgggaagtc catacaacca gaaaatcttg aatatcgaat tatgttgtcc
      481 gttcacggta gccagcattc tggaatgatt gtcaatgata ctgggcacga aactgatgag
      541 aatcgagcga aagtagagat cacgcccaac tcacctcgcg cggaagcgac gttgggaggg
      601 tttggttccc tgggactgga ttgcgagcct agaactggac tcgacttttc cgacctgtac
      661 tatttgacaa tgaataataa gcattggttg gtccacaagg agtggttcca tgatattccg
      721 ctgccctggc atgcgggcgc ggacactgga actccgcatt ggaataacaa agaagccctc
      781 gttgagttta aagatgccca cgccaagagg caaacagttg tcgtattggg ctcccaggaa
      841 ggttgggtgc accgggcttt ggccggagcc ctcgaagcag aaatggacgg agcgaaaggt
      901 cgcctttggc ccggacacct caagtgcagg ctgaagatgg acaaactccg actcaaaggg
      961 gtttcctata gcctttgcac ggcagccttt acattcacaa aaatccccgc cgaaacgttg
     1021 cacggaactg tgaccgtgga ggtgcagtat gccggcacgg acggcccttg caaagtcccg
     1081 gctcaaatgg ctgtagatat gcaaacattg accccggtcg gacggctcat tactgccaat
     1141 cctgttatca cagaatccac agaaaactca aagatgatgc ttgagctcga ccctcccttc
     1201 ggagactcat atattgtgat tggggtaggt gaaaagaaaa ttactcatca ttggcatcgc
     1261 tctggtggca gcagcggcgg cagccatcac caccatcatc accatcat
//
