LOCUS       sequence-429478-        4578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression tetramerization domain (TET) and C-terminal 
            negative regulatory domain (CTD) of human TP53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: EF1a-hP53DD (TET+CTD)
FEATURES             Location/Qualifiers
     source          1..4578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        328..432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(433..501,502..1293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(651..670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1464..2052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1953..1972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2206..2223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2291..2425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2315..2334)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2369..2388
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2431..2626)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2445..2580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2685..2706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2721..2726,2727..2744,2745..2751)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2759..2775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2764..2786
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2783..2799
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2783..2799
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3142..3161
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3160..3215
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3261..3280
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     promoter        complement(join(3662..4578,1..266))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(3671..4578,1..35)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3694..3714)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc aaacccgttg
       61 cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc accctcggga
      121 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
      181 gcaccggttc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
      241 tctgcccact gacgggcacc ggagcctcac gcgatgatct agtcaataat caatgtcgac
      301 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      361 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      421 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      481 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      541 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      601 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      661 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      721 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      781 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      841 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      901 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      961 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1021 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1081 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1141 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1201 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1261 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1321 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1441 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1501 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1561 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1621 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1681 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1741 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1801 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1861 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1921 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1981 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2041 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2101 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2161 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2221 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2281 taatgcagcg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2341 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2401 cattataagc tgcaataaac aagttagctt tttgcaaaag cctaggcctc caaaaaagcc
     2461 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     2521 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     2581 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcggat ccgctggcac
     2641 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2701 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2761 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttggg
     2821 ctgcaggtcg agggatctcc ataagagaag agggacagct atgactggga gtagtcagga
     2881 gaggaggaaa aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa
     2941 aaataacaca aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa
     3001 taaggaatgg acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt
     3061 gttcatggca gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg
     3121 atgttttaaa tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca
     3181 acacactatt gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg
     3241 cttcatgatg tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc
     3301 cagggcattg gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgcg
     3361 gccgctagcg ggtttaaaca acttggcgcg cctaaacacc ggtcagtctg agtcaggccc
     3421 ttctgtcttg aacatgagtt ttttatggcg ggaggtagac tgaccctttt tggacttcag
     3481 gtggctggag tgagccctgc tcccccctgg ctccttccca gcctgggcat ccttgagttc
     3541 caaggcctca ttcagctctc ggaacatctc gaagcgctca cgcccacgga tctgaagggt
     3601 gaaatattct ccatccagca tggtggcccc gggcccgcgg taccgtcgac tgcagaattc
     3661 ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga
     3721 accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg
     3781 gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa aacccctccc
     3841 cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg
     3901 cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt
     3961 gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt gagctccctg
     4021 cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac cgggccagcc
     4081 ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga gcaggccggc
     4141 cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg
     4201 aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa accgaaatac
     4261 cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag
     4321 caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga caggcgcgaa
     4381 ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca actcaagcac
     4441 gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa
     4501 gctcgggatc aagaatcacg tactgcagcc aggggcgtgg aagtaattca aggcacgcaa
     4561 gggccataac ccgtaaag
//
