LOCUS       sequence-429497-       10039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of the CRISPRi system for genome editing in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Zhang H, Ma J, Wu Z, Chen X, Qian Y, Chen W, Wang Z, Zhang Y, Zhu H,
            Huang X, Ji Q
  TITLE     BacPE: a versatile prime-editing platform in bacteria by inhibiting 
            DNA exonucleases.
  JOURNAL   Nat Commun. 2024 Jan 27;15(1):825. doi: 10.1038/s41467-024-45114-4.
  PUBMED    38280845
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-01-27"; volume: "15(1):825. doi"; pages: " 
            10.1038/s41467-024-45114-4"
COMMENT     Sequence Label: pBbS8c_ddCpf1_final
FEATURES             Location/Qualifiers
     source          1..10039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(106..111,112..128,129..135)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    143..159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      270..341
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      357..384
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        join(571..576,577..593,594..600)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    608..624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      735..806
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      822..849
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        join(1011..1016,1017..1033,1034..1040)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1048..1064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1175..1246
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1262..1289
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        join(1535..1540,1541..1557,1558..1564)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1572..1588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1699..1770
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1786..1813
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2192..3142)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3904..3998)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4019..4678)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4593..4612
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4679..4781)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(4811..5689)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5716..6000
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5923..5942
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     regulatory      9756..9765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagtccatgt gaccttcacc ctcatggacc gcaaaaaacc ccgcttcggc ggggtttttt
       61 cgctgcgcaa ctttggtaac ttgatgtcta cacctaaatg agctgttgac aattaatcat
      121 ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcaatttct actcttgtag
      181 atacgcaacc agcttccggt cactgttttt ttgaagcttg ggcccgaaca aaaactcgga
      241 tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
      301 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      361 caccttcggg tgggcctttc tgcgtttata cctagggtac gggttttgct gcccgcaaac
      421 gggctgttct ggtgttgcta gtttgttatc agaatcgcag atccgtagtc catgtgacct
      481 tcaccctcat ggaccgcaaa aaaccccgct tcggcggggt tttttcgctg cgcaactttg
      541 gtaacttgat gtctacacct aaatgagctg ttgacaatta atcatccggc tcgtataatg
      601 tgtggaattg tgagcggata acaatttcaa tttctactct tgtagatacc taatgatgaa
      661 tgacggtaag tttttttgaa gcttgggccc gaacaaaaac tcggatccaa actcgagtaa
      721 ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      781 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      841 ctttctgcgt ttatacctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt
      901 tccgctagtc catgtgacct tcaccctcat ggaccgcaaa aaaccccgct tcggcggggt
      961 tttttcgctg cgcaactttg gtaacttgat gtctacacct aaatgagctg ttgacaatta
     1021 atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcaa tttctactct
     1081 tgtagatacg attacgaaac ctttggcacg tttttttgaa gcttgggccc gaacaaaaac
     1141 tcggatccaa actcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa
     1201 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1261 tggctcacct tcgggtgggc ctttctgcgt ttatacctag ggtacgggtt ttgctgcccg
     1321 caaacgggct gttctggtgt tggctgaaag cgctatttct tccagaattg ccatgatttt
     1381 ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgacct agtccatgtg
     1441 accttcaccc tcatggaccg caaaaaaccc cgcttcggcg gggttttttc gctgcgcaac
     1501 tttggtaact tgatgtctac acctaaatga gctgttgaca attaatcatc cggctcgtat
     1561 aatgtgtgga attgtgagcg gataacaatt tcaatttcta ctcttgtaga ttggcctggg
     1621 atcaagtaca gcaatttttt tgaagcttgg gcccgaacaa aaactcggat ccaaactcga
     1681 gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1741 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1801 gggcctttct gcgtttatac ctagggtacg ggttttgctg cccgcaaacg ggctgttctg
     1861 gtgttctcgc aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc
     1921 aatttcatgt tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg
     1981 ctgttcatct gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac
     2041 tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag
     2101 ttttcccttt gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc
     2161 ttcactgata gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt
     2221 tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact
     2281 tactttgcat gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta
     2341 aagcatcgtg tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg
     2401 ttggtatttt gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca
     2461 tttgtctatc tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa
     2521 ccaccaattt catattgctg taagtgttta aatctttact tattggtttc aaaacccatt
     2581 ggttaagcct tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat
     2641 caaggctaat ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc
     2701 actcataaat cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt
     2761 ttatgaattt ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta
     2821 atttttcgct tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca
     2881 aaggattcct gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac
     2941 cataagcatt ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata
     3001 ccgtccgttc tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata
     3061 aaattagctt ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt
     3121 tgaaaacaac taattcagac atacatctca attggtctag gtgattttaa tcactatacc
     3181 aattgagatg ggctagtcaa tgataattac tagtcctttt cccgggtgat ctgggtatct
     3241 gtaaattctg ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt
     3301 ccgctagacc tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa
     3361 agaaagaata aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt
     3421 agtcagttcc gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca
     3481 gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata
     3541 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc
     3601 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg
     3661 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt
     3721 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc
     3781 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa
     3841 tgcacccagt aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct
     3901 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     3961 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     4021 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     4081 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     4141 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     4201 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     4261 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     4321 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     4381 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     4441 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     4501 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     4561 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     4621 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     4681 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     4741 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga
     4801 tatcgacgtc ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
     4861 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
     4921 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
     4981 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
     5041 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
     5101 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
     5161 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
     5221 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
     5281 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
     5341 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
     5401 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
     5461 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
     5521 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
     5581 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     5641 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     5701 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     5761 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     5821 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     5881 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     5941 ccataagatt agcggattct acctgacgct ttttatcgca actctctact gtttctccat
     6001 acccgttttt ttgggaattc aaaagatcta caccgtcata aaatgcctcg catgtcagtg
     6061 ttattctctt gcgcggttca taatccatga aactgttttt atgtaggagg tcgtgatgac
     6121 ccagtttgaa ggttttacca atctttatca ggtaagcaag acgctgagat ttgagctgat
     6181 tccccagggc aaaacactga aacacattca agagcaaggc ttcattgaag aggataaggc
     6241 ccgaaacgac cattataaag aacttaaacc gattatcgat cgcatctaca agacgtatgc
     6301 agaccagtgt ctgcaactgg tgcagttaga ttgggaaaat ttaagcgcgg ctatagactc
     6361 ttaccgtaag gagaaaacag aagaaactcg taatgcatta atcgaggagc aggctacata
     6421 ccgcaatgca attcatgact attttattgg taggacagat aatctaacgg acgccatcaa
     6481 caagcgtcat gccgaaatat acaaaggtct gtttaaagcg gagctgttca acggtaaagt
     6541 attaaagcag ttaggtaccg taacgaccac ggaacatgaa aatgccttgc tgcgttcttt
     6601 tgataaattc actacgtatt tttccgggtt ctacgagaat cgcaagaacg tgtttagcgc
     6661 agaagatatt agtacggcca ttccgcatcg tattgttcaa gacaattttc cgaaatttaa
     6721 agaaaattgt catattttta ctcgtctgat cacagcagtg ccatcactgc gagaacattt
     6781 tgaaaatgtt aagaaggcga ttggcatttt cgtgtcaacc agcattgaag aagtgttttc
     6841 attcccgttc tataatcagc ttcttaccca gacacagatt gacctatata atcagctctt
     6901 aggtggtatt agccgagaag ctggaacgga aaagataaaa ggcctgaatg aggtgctgaa
     6961 cctggcaatt caaaagaacg atgaaacagc gcacattatt gcatctctcc cccatcgatt
     7021 tataccgctc tttaaacaaa ttctgtccga ccgcaacaca ctgagcttta tcctggagga
     7081 atttaaaagc gatgaagaag taattcagag cttctgcaaa tataaaacgt tactgcgcaa
     7141 tgaaaacgtc ctggaaacgg ctgaggccct ctttaacgaa ctgaacagca ttgatctcac
     7201 ccatattttt atttcacata aaaagctgga aactatcagc agcgcgctgt gcgaccattg
     7261 ggacacgctg agaaatgccc tgtatgaacg tcgaatttcc gagctgaccg gaaagatcac
     7321 caagtcagct aaagagaaag tccagcgctc cctcaaacat gaagatatta acttacaaga
     7381 gattatttcg gcggccggta aggaactgtc ggaggctttc aaacagaaga caagcgaaat
     7441 cctctcacat gcgcacgcgg ccctagatca gcctctgccg acgacgctga aaaagcagga
     7501 agagaaagaa atcctgaaat ctcaattaga ttctctactg ggactgtacc acttgctgga
     7561 ctggtttgcg gtggacgagt ctaatgaggt ggaccctgaa tttagcgcac gcctgaccgg
     7621 tataaaattg gagatggaac cgtcgttgag cttttacaac aaagcacgca actatgcgac
     7681 caaaaagcct tattcagtgg aaaaattcaa attgaacttc caaatgccga ccctagcgtc
     7741 aggttgggat gtcaacaaag agaagaacaa cggtgcgatc ttatttgtga aaaacggcct
     7801 gtattatctg ggcattatgc cgaagcagaa aggccgttac aaggcactgt cattcgaacc
     7861 gacggagaag accagtgaag ggtttgataa aatgtattac gactactttc cggatgccgc
     7921 caaaatgatt ccgaagtgtt cgacccagct gaaagcggtg accgcgcatt ttcagacaca
     7981 caccaccccg atcctgcttt caaataactt tatagaaccg ctggaaataa ccaaagaaat
     8041 ttacgacctg aacaatccgg aaaaagaacc gaagaaattt cagaccgcct acgcaaagaa
     8101 aaccggagat caaaaaggtt accgcgaagc cctgtgtaaa tggattgatt ttacgcgcga
     8161 ttttctcagc aaatacacca agaccacttc aatagatttg agttcgctgc gtccgagctc
     8221 ccagtataaa gacctgggcg aatattatgc cgagctgaac ccgttactgt accacattag
     8281 cttccagcgc attgcggaaa aagaaatcat ggatgcagtc gaaactggaa agctttatct
     8341 gttccagatt tacaacaagg attttgcgaa aggccatcac ggcaaaccga atctacatac
     8401 tctctattgg actggcctgt tctctcccga aaacttggcc aagacctcaa ttaaactcaa
     8461 tggccaagcc gagctgttct accgtccgaa atcgcgcatg aagcggatgg cccatcgtct
     8521 tggggagaaa atgctcaata agaaactgaa ggatcagaaa acaccgatcc ctgacacgct
     8581 gtatcaggaa ctttacgact atgtcaatca ccgtctgagt cacgatctgt cggacgaagc
     8641 acgggcctta ctgccgaacg taataaccaa agaggtgtca cacgagatca ttaaggatcg
     8701 acgttttacc tccgacaaat tctttttcca cgtaccgatt acccttaatt accaggcggc
     8761 gaactcaccc tcgaaattta accagcgcgt aaacgcgtac ctgaaagagc acccggaaac
     8821 ccctattatt gggattgacc gtggcgaacg caatcttatc tatataaccg taatcgactc
     8881 taccggcaag attttagaac agcgtagcct aaacaccatt cagcaatttg attaccagaa
     8941 aaagttagat aatcgggaaa aagaacgtgt ggcggcacgg caggcttggt cggtagttgg
     9001 gacgattaag gatcttaaac aaggttattt atcccaggtt atacacgaaa tcgtagactt
     9061 aatgatacat tatcaagccg ttgtggtgct ggccaacctg aacttcggat ttaagtcaaa
     9121 gcggacgggc attgccgaaa aggcggtgta tcagcagttc gagaagatgc tgatcgataa
     9181 actcaattgc ttagtactaa aagattatcc cgctgaaaag gtaggtggcg ttctcaaccc
     9241 gtaccagctt acagatcaat ttaccagctt tgcgaagatg ggtacacaga gcggttttct
     9301 gttttatgtt cccgcaccgt ataccagcaa aatcgatccc ctgaccggct ttgttgatcc
     9361 ttttgtatgg aaaaccatca aaaaccacga aagccgcaaa catttcctgg agggctttga
     9421 ttttcttcac tacgatgtga aaacaggcga ttttattctg cacttcaaaa tgaatcgcaa
     9481 tcttagcttt cagcgcggtc tgcctgggtt tatgccggcg tgggatattg tgttcgaaaa
     9541 gaatgaaacg cagttcgacg ccaaaggcac accgtttatt gccgggaaac gtatagtacc
     9601 ggtgatcgaa aatcatcggt ttacaggccg ttatcgcgac ctgtacccgg ctaatgagct
     9661 gattgcactg ctcgaagaaa agggaattgt atttcgtgac ggttccaata ttctgcctaa
     9721 actgttggaa aatgatgact cgcacgcgat tgataccatg gtggctctta tccgcagcgt
     9781 tcttcaaatg cgtaactcta acgcggcgac gggggaagat tatattaaca gcccggtacg
     9841 cgatctcaac ggcgtctgtt ttgatagtcg cttccagaac cccgaatggc cgatggacgc
     9901 ggacgccaac ggagcatacc atattgcgct gaaaggccag ctgttactga atcatctcaa
     9961 agaatccaaa gacctcaaac tacagaatgg catttcaaat caggattggc tcgcgtatat
    10021 tcaggaactg cgcaattga
//
