LOCUS       sequence-429500-       11440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses presynaptic non-responsive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 11440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..11440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..85)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        257..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        638..836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     791..811
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             854..1034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1081..1206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1699..1932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2117..2161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2459..2576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2621..3909
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3919..3928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5118..5139
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5242..5263)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6151..6739
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6204..6224)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6622..6633)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6742..6758)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6743..6759)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7264..7444
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7470..7487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7476..7700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7746..8174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7833..7852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8043..8064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8183..8203)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8188..8517
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8368..8503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8430..8449
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8565..8612
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8631..9005
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9135..9256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9172..9191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9226..9245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9305..9321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9305..9321)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9318..9340)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9329..9345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9353..9359,9360..9377,9378..9383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9398..9419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9536..9553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9707..10295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9787..9806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10466..11257,11258..11326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11089..11108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11327..11431)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
       61 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
      121 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
      181 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      241 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      301 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
      841 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1021 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     1081 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     1141 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1201 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1261 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1321 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1381 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1441 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1501 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1561 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1621 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1681 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1741 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1801 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1861 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1921 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1981 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     2041 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     2101 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     2161 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2221 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2281 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2341 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2401 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2461 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2521 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2581 tttattacag ggacagcaga gatccagttt ggttaattaa cattatggcc ttaggtcact
     2641 tcatctccat ggggttcttc ttctgatttt ctagaaaatg agatgggggt gcagagagct
     2701 tcctcagtga cctgcccagg gtcacatcag aaatgtcaga gctagaactt gaactcagat
     2761 tactaatctt aaattccatg ccttgggggc atgcaagtac gatatacaga aggagtgaac
     2821 tcattagggc agatgaccaa tgagtttagg aaagaagagt ccagggcagg gtacatctac
     2881 accacccgcc cagccctggg tgagtccagc cacgttcacc tcattatagt tgcctctctc
     2941 cagtcctacc ttgacgggaa gcacaagcag aaactgggac aggagcccca ggagaccaaa
     3001 tcttcatggt ccctctggga ggatgggtgg ggagagctgt ggcagaggcc tcaggagggg
     3061 ccctgctgct cagtggtgac agataggggt gagaaagcag acagagtcat tccgtcagca
     3121 ttctgggtct gtttggtact tcttctcacg ctaaggtggc ggtgtgatat gcacaatggc
     3181 taaaaagcag ggagagctgg aaagaaacaa ggacagagac agaggccaag tcaaccagac
     3241 caattcccag aggaagcaaa gaaaccatta cagagactac aagggggaag ggaaggagag
     3301 atgaattagc ttcccctgta aaccttagaa cccagctgtt gccagggcaa cggggcaata
     3361 cctgtctctt cagaggagat gaagttgcca gggtaactac atcctgtctt tctcaaggac
     3421 catcccagaa tgtggcaccc actagccgtt accatagcaa ctgcctcttt gccccactta
     3481 atcccatccc gtctgttaaa agggccctat agttggaggt gggggaggta ggaagagcga
     3541 tgatcacttg tggactaagt ttgttcgcat ccccttctcc aaccccctca gtacatcacc
     3601 ctgggggaac agggtccact tgctcctggg cccacacagt cctgcagtat tgtgtatata
     3661 aggccagggc aaagaggagc aggttttaaa gtgaaaggca ggcaggtgtt ggggaggcag
     3721 ttaccggggc aacgggaaca gggcgtttcg gaggtggttg ccatggggac ctggatgctg
     3781 acgaaggctc gcgaggctgt gagcagccac agtgccctgc tcagaagccc caagctcgtc
     3841 agtcaagccg gttctccgtt tgcactcagg agcacgggca ggcgagtggc ccctagttct
     3901 gggggcagcg ggggatccgc caccatggac gtggtgaatc agctggtggc tgggggtcag
     3961 ttccgggtgg tcaaggagcc ccttggcttc gtgaaggtgc tgcagtgggt ctttgccatc
     4021 ttcgcctttg ctacgtgtgg cagctacacc ggggagcttc ggctgagcgt ggagtgtgcc
     4081 aacaagacgg agagtgccct caacatcgaa gttgaattcg agtacccctt caggctgcac
     4141 caagtgtact ttgatgcacc ctcctgcgtc aaagggggca ctaccaagat cttcctggtt
     4201 ggggactact cctcgtcggc tgaattcttt gtcaccgtgg ctgtgtttgc cttcctctac
     4261 tccatggggg ccctggccac ctacatcttc ctgcagaaca agtaccgaga gaacaacaaa
     4321 gggcctatga tggactttct ggctacagcc gtgttcgctt tcatgtggct agttagttca
     4381 tcagcctggg ccaaaggcct gtccgatgtg aagatggcca cggacccaga gaacattatc
     4441 aaggagatgc ccatgtgccg ccagacaggg aacacatgca aggaactgag ggaccctgtg
     4501 acttcaggac tcaacacctc agtggtgttt ggcttcctga acctggtgct ctgggttggc
     4561 aacttatggt tcgtgttcaa ggagacaggc tgggcagccc cattcatgcg cgcacctcca
     4621 ggcgccccgg aaaagcaacc agcacctggc gatgcctacg gcgatgcggg ctacgggcag
     4681 ggccccggag gctatgggcc ccaggactcc tacgggcctc agggtggtta tcaacccgat
     4741 tacgggcagc cagccagcgg tggcggtggc tacgggcctc agggcgacta tgggcagcaa
     4801 ggctatggcc aacagggtgc gcccacctcc ttctccaatc agatgggatc cactagtaac
     4861 ggccgccagt gtgctggaat tctgcagata tcgatgggca gatctgtcgg gaacgtctat
     4921 atcaccgccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacgtg
     4981 gaggacggca gcatgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5041 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgtgctgag caaagaccct
     5101 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5161 ggcatggacg agctgtacaa gggcggtacc ggagggagca tgagcaaggg cgaggagctg
     5221 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5281 agcgtgcgcg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     5341 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     5401 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5461 atgcccgaag gctacgtcca ggagcgcacc atcagcttca aggacgacgg cacctacaag
     5521 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5581 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctttctgcag
     5641 ggcggaggct cgggcggagg tgggtcgggt ggcggcggat caatggcaaa cctggacaag
     5701 atgctgaaca ccaccgtgac cgaggtgcgc aagttcctgc aagcggacag agtgtgcgtg
     5761 ttcaagttcg aggaagatta ctccggcacc gtctcgcacg aagccgtgga cgacagatgg
     5821 attagcatcc tgaagaccca ggtgcaggac agatacttca tggaaaccag aggcgaggaa
     5881 tacgtccacg gcagatacca ggccatcgcc gacatctaca cagccaatct ggtcgagtgc
     5941 tacagagacc tgctgatcga gtttcaggtg cgggccattc tggctgtccc catcctgcaa
     6001 ggcaagaagc tgtggggcct gctggtggcc caccaactgg ccggccctcg ggagtggcag
     6061 acctgggaaa tcgacttcct gaaacagcaa gccgtggtga tgggcatcgc catccagcag
     6121 agctaggaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     6181 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6241 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6301 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6361 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6421 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6481 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6541 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6601 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6661 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6721 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     6781 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     6841 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     6901 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6961 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7021 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     7081 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     7141 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     7201 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     7261 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7321 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7381 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7441 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7501 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7561 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7621 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7681 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7741 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7801 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7861 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7921 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7981 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8041 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8101 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8161 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8341 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8401 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     8461 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8521 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     8581 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     8641 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     8701 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     8761 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     8821 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     8881 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     8941 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     9001 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9061 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9121 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9241 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9301 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9361 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9421 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9481 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9541 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9601 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9901 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10141 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10201 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc
//
