LOCUS       sequence-429505-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of CREB1 domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Martinez-Yamout MA, Nasir I, Shnitkind S, Ellis JP, Berlow RB, Kroon
            G, Deniz AA, Dyson HJ, Wright PE
  TITLE     Glutamine-rich regions of the disordered CREB transactivation domain
            mediate dynamic intra- and intermolecular interactions.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2313835120. doi: 
            10.1073/pnas.2313835120. Epub 2023 Nov 14.
  PUBMED    37971402
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-11-21"; volume: "120(47):e2313835120. 
            doi"; pages: " 10.1073/pnas.2313835120. Epub 2023 Nov 14"
COMMENT     Sequence Label: H6Gb1-CREB(1-280) A50C,C90A
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..201
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             226..246
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggccatc atcatcatca tcacagcagc ggcatgactt acaaactgat ccttaacggt
       61 aaaactttga aaggcgaaac cactaccgaa gctgttgatg ctgcgactgc agaaaaagtt
      121 ttcaaacagt acgctaacga caacggtgtt gacggtgaat ggacttacga cgatgcgact
      181 aagaccttca ccgttactga agaattcagc agcggcagca gcggcgaaaa cctgtatttt
      241 cagggctccc atatgaccat ggactctgga gcagacaacc agcagagtgg agatgctgct
      301 gtaacagaag ctgaaagtca acaaatgaca gttcaagccc agccacagat tgccacatta
      361 gcccaggtat ccatgccagc agctcatgcg acgtcatctt gtcccactgt aaccttagtg
      421 cagctgccca atgggcagac agtccaggtc catggggtca tccaggcggc ccagccatca
      481 gttattcagt ctccacaagt ccaaacagtt cagtcttccg ctaaggactt aaaaagactt
      541 ttctccggaa ctcagatttc aactattgca gaaagtgaag attcacagga gtctgtggat
      601 agtgtaactg attcccaaaa acgaagggaa atcctttcaa ggaggccttc ctacaggaaa
      661 attttgaatg acttatcttc tgatgcacca ggggtgccaa ggattgaaga agaaaaatca
      721 gaagaagaga cttcagcccc tgccatcacc actgtaacag tgccaacccc gatttaccaa
      781 actagcagtg ggcagtatat tgccattacc cagggaggag caatacagct ggctaacaat
      841 ggtaccgatg gggtacaggg cctgcagaca ttaaccatga ccaatgcagc tgccactcag
      901 ccgggtacta ccattctaca atatgcacag accactgatg gacagcagat tctagtgccc
      961 agcaaccaag ttgttgttca agctgcctct ggtgatgtac aaacatacca gattcgcaca
     1021 gcacccacta gcaccattgc ccctggagtt gttatggcgt cctccccagc acttcctaca
     1081 cagcctgctg aataataga
//
