LOCUS       sequence-429508-        4205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U3 promoter driven expression of the telomerase RNA with a G414C CAB
            box mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Klump BM, Perez GI, Patrick EM, Adams-Boone K, Cohen SB, Han L, Yu 
            K, Schmidt JC
  TITLE     TCAB1 prevents nucleolar accumulation of the telomerase RNA to 
            facilitate telomerase assembly.
  JOURNAL   Cell Rep. 2023 Jun 1;42(6):112577. doi: 
            10.1016/j.celrep.2023.112577.
  PUBMED    37267110
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-1"; volume: "42(6):112577. doi"; pages: " 
            10.1016/j.celrep.2023.112577"
COMMENT     Sequence Label: pSuper TR G414C
FEATURES             Location/Qualifiers
     source          1..4205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    297..418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(334..353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     388..407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(445..461)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(446..462)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(486..505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(487..505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(515..532)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(515..531)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(524..546)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(672..1128)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(810..831)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1022..1041
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1155..1259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1260..1328,1329..2120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1478..1497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2291..2879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2780..2799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3033..3050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3167..3188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3203..3208,3209..3226,3227..3233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3241..3257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3246..3268
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3265..3281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3265..3281
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3300..3320
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3302..3320
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3357..3373
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3357..3373
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctgtgggacg tgcacccagg actcggctca cacatgcagt tcgctttcct gttggtgggg
       61 ggaacgccga tcgtgcgcat ccgtcacccc tcgccggcaa tgggggcttg tgaaccccca
      121 aacctgactg actgggccag tgtgctgcaa attggcagga gactagtgga tccaccggat
      181 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      241 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
      421 gcgtaaattg taagccaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca
      481 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
      541 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      601 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      661 atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      721 aatcagctca tttttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
      781 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
      841 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
      901 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
      961 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     1021 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     1081 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     1141 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1501 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1561 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1621 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1681 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1741 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1801 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1861 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1921 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1981 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2041 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2101 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2161 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2221 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2401 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     3181 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     3241 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     3301 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta
     3361 gaactagtgg atcccccggg ctgcaggaat tcagatctta aggaccagct tctttgggag
     3421 agaacagacg caggggcggg agggaaaaag ggagaggcag acgtcacttc cccttggcgg
     3481 ctctggcagc agattggtcg gttgagtggc agaaaggcag acggggactg ggcaaggcac
     3541 tgtcggtgac atcacggaca gggcgacttc tatgtagatg aggcagcgca gaggctgctg
     3601 cttcgccact tgctgcttcg ccacgaagga gttcccgtgc cctgggagcg ggttcaggac
     3661 cgcggatcgg aagtgagaat cccagctgtg tgtcagggct ggaaagggct cgggagtgcg
     3721 cggggcaagt gaccgtgtgt gtaaagagtg aggcgtatga ggctgtgtcg gggcagagcc
     3781 cgaagatcat cgggttgcgg agggtgggcc tgggaggggt ggtggccatt ttttgtctaa
     3841 ccctaactga gaagggcgta ggcgccgtgc ttttgctccc cgcgcgctgt ttttctcgct
     3901 gactttcagc gggcggaaaa gcctcggcct gccgccttcc accgttcatt ctagagcaaa
     3961 caaaaaatgt cagctgctgg cccgttcgcc cctcccgggg acctgcggcg ggtcgcctgc
     4021 ccagcccccg aaccccgcct ggaggccgcg gtcggcccgg ggcttctccg gaggcaccca
     4081 ctgccaccgc gaagagttgg gctctgtcag ccgcgggtct ctcgggggcg agggcgaggt
     4141 tcaggccttt caggccgcag gaagaggaac ggagcgagtc cccgcgcgcg gcgcgattcc
     4201 ctgac
//
