LOCUS       sequence-429510-       14900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express T. aq RNA Polymerase subunits in E. coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Hertz LM, White EN, Kuznedelov K, Cheng L, Yu AM, Kakkaramadam R, 
            Severinov K, Chen A, Lucks JB
  TITLE     The effect of pseudoknot base pairing on cotranscriptional 
            structural switching of the fluoride riboswitch.
  JOURNAL   Nucleic Acids Res. 2024 May 8;52(8):4466-4482. doi: 
            10.1093/nar/gkae231.
  PUBMED    38567721
REFERENCE   2  (bases 1 to 14900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2024-05-8- 8"; volume: "52"; issue: "8";
            pages: "4466-4482"
COMMENT     Sequence Label: T. aq RNA Polymerase
FEATURES             Location/Qualifiers
     source          1..14900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1283..1302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1367..1386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2339..2358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2499..2521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4559..4578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4881..4900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        6070..6088
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6089..6113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6128..6150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9335..9346
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9587..9605
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9606..9630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             9645..9667
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9686..9703
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             9713..9730
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     promoter        14331..14349
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    14350..14374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             14389..14411
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             14744..14761
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(14814..14832)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      14828..14875
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgttggaga gcaagctgaa agccccggtc
     5101 ttcacggcgc gcacccaggg gcgccactac ggcgagttcg tcctggagcc cctagaaagg
     5161 gggtttgggg tcaccctggg gaaccctctc cgccgcatcc tcctctcctc catccccggg
     5221 accgcggtca ccagcgtcta cattgaggac gtcctccacg agttctccac cattcccggg
     5281 gtgaaggagg acgtggtgga gatcatcctg aacctgaagg agctggtggt gcgcttcctg
     5341 gaccccaaga tggcgtccac caccctgatc ctcagggcgg agggccccaa ggaggtgagg
     5401 gcgggcgact tcaccccctc cgccgacgtg gagatcatga accccgacct ccacatcgcc
     5461 accctcgagg agggcgggaa gctctacatg gaggtgcggg tggaccgggg ggtggggtac
     5521 gtgccggccg agcgccacgg catcaaggac cgcatcaacg ccatccctgt ggatgccatc
     5581 ttctccccgg tgcgccgggt ggccttccag gtggaggaca cccgcctggg ccagcgcacg
     5641 gacctggaca agctcacctt gaggatctgg accgacggct ccgtcacccc cctcgaggcc
     5701 ctgaaccagg ccgtggccat cctgaaggag cacctgaact acttcgccaa ccccgaggcc
     5761 tcccttctgc ccacccccga ggtgtcgaag ggggagaagc gggagagcgc cgaggaggac
     5821 ctggacctgc ccttggagga gcttgggctt tccacccggg tcctccacag cctcaaggag
     5881 gaggggattg agtccgtgcg cgcccttctg gccctcaacc tcaaggacct gaggaacatc
     5941 cccggcatcg gggagcggag cctggaggag atccgccagg ccctggccaa gaagggcttc
     6001 accctaaagg agtgaattat ggcggaaccg ggcattgaca agctcttcgg catctcgatc
     6061 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     6121 aaataatttt gtttaacttt aagaaggaga tatacatatg gagattaagc ggttcggtcg
     6181 catccgagag gtcattcccc ttccccccct tacggaaatc caggtggagt cctataagaa
     6241 ggcgctccag gcggacgttc cccctgaaaa gcgggagaac gtgggcatcc aggccgcctt
     6301 caaggagacc ttccctatag aggaggggga caagggcaag ggtggtctgg ttctggactt
     6361 cctggagtac cgcatcggcg accccccctt ctcccaggac gagtgccggg agaaggacct
     6421 cacctaccag gctccccttt acgcccggct ccagctcatc cacaaggaca ccgggctcat
     6481 caaggaggac gaggtcttcc tgggccacct gcccctgatg accgaggacg gctccttcat
     6541 catcaacggg gccgaccggg tcatcgtctc ccagatccac cgctcccccg gggtctactt
     6601 cacccccgac cccgcccgcc ccggcaggta catcgccagc atcatccccc tgcccaagcg
     6661 ggggccctgg attgacctcg aggtggaggc cagcggcgtg gtgaccatga aggtcaacaa
     6721 gcgcaagttc cccctggtcc tcctcctcag ggtcctgggc tacgaccagg agaccctggt
     6781 gcgggagctt tccgcctacg gggacctggt ccagggcctc ctggacgagg cggttttggc
     6841 catgcgcccc gaggaggcca tggtccgcct gttcaccctc ctccgccccg gtgacccccc
     6901 caagaaggac aaggccctgg cctacctctt tggccttctg gccgacccca agcgctacga
     6961 cctgggagag gcgggccgct acaaggcaga ggagaagctg ggcgtgggcc tttcggggcg
     7021 caccctggtc cggtttgagg acggcgagtt caaggacgag gtcttcctgc ccacgctccg
     7081 ctacctcttc gccctaacgg ccggggttcc cggccacgag gtggacgaca ttgaccacct
     7141 gggcaaccgg cgaatccgca ccgtgggcga gctcatggcg gaccagttcc gcgtgggcct
     7201 ggccaggctg gcccgagggg tgcgggagcg catggtcatg ggctcccccg acaccctcac
     7261 ccccgccaag ctggtcaaca gccgccccct ggaggccgcc ttgcgggagt ttttcagccg
     7321 gagccagctc tcccagttca aggacgagac caaccccctc tcctccctga ggcacaaaag
     7381 gcgcatttcc gccctgggcc ccgggggcct tacccgggag cgggcggggt ttgacgtgcg
     7441 cgacgtgcac cgcacccact acgggcgcat ctgcccggtg gagacgccgg aaggggccaa
     7501 catcggcctc atcacctccc tggccgccta cgcccgggtg gacgccttgg gcttcatccg
     7561 caccccctac cgccgggtca agaacggcgt ggtcacggag gaggtggtct acatgaccgc
     7621 ctccgaggag gaccgctaca ccatcgccca ggccaacacc cccctcgagg gcgaccgcat
     7681 cgccaccgac cgggtggtgg ccaggcgcag gggggagccc gtcatcgtgg ctccggagga
     7741 ggtggagttc atggacgtct cccccaagca ggtcttctct ctcaacacca acctcattcc
     7801 cttcctggag cacgacgacg ccaaccgggc cctcatgggc tccaacatgc agacccaggc
     7861 cgtgcccctc atccgggccc aggccccggt ggtcatgacg ggcctggagg agcgcgtggt
     7921 gcgggactcc ctggccgccc tctacgccga ggaggacggg gaggtggtca aggtggacgg
     7981 caccaggatc gccgtccgct atgaggacgg ccgcctggtg gagcaccctt tgcgccgcta
     8041 tgcccgctcc aaccaaggca ccgccttcga ccagcggccc cgggtcaggg tgggccagcg
     8101 ggtgaagaag ggggacctcc tggcggacgg ccccgcctcg gaagagggct ttctggccct
     8161 ggggcagaac gtcctggtgg ccatcatgcc ctttgacggg tacaactttg aggacgccat
     8221 cgtcatcagc gaggagctcc tcaagcggga cttctacacc tccatccaca ttgagcgcta
     8281 cgagattgag gcccgggaca ccaagctggg ccccgagcgt attacccgtg acatccccca
     8341 cctctccgag gccgcccttc gcgacctgga cgaggagggc atcgtccgca tcggggccga
     8401 ggtcaagccc ggcgacatcc tggtggggcg caccagcttc aagggcgagc aggaaccttc
     8461 ccccgaggag cgcctcctgc gctccatctt cggggagaag gccagggacg tgaaggacac
     8521 ctccctgagg gtcccccccg gggaaggggg gatcgtggtg gggcgcctgc gccttaggcg
     8581 gggcgatccc ggggtggagc tgaagcccgg ggtgcgggag gtggtccggg tcttcgtggc
     8641 ccagaagcgc aagctccagg tgggggacaa gctggccaac cgccacggca acaagggcgt
     8701 ggtggccaag atcctccccg tggaggacat gccccacctg cccgacggca cccccgtgga
     8761 cgtgatcctg aaccccctgg gcgtgcccag ccggatgaac ctgggccaga tcctggagac
     8821 ccacctgggc ctggcgggct acttcctggg ccagcgctac atctcccccg tctttgacgg
     8881 ggccacggag cccgagatca aggagcttct ggccgaggcc ttcaacctct acttcggcaa
     8941 gcgccagggc gagggcttcg gcgtggacaa gcgggagaag gaggtcctgg cccgggcgga
     9001 gaagctgggc ctggtttccc cgggcaagag ccccgaggag caactcaaag agctcttcgt
     9061 ccagggcaag gtggtcctct acgatggccg cacgggggag ccctttgagg ggcccatcgt
     9121 ggtggggcag atgttcatca tgaagctcta ccacatggtg gaggacaaga tgcacgcccg
     9181 ctccactggc ccctactccc tcatcaccca gcagcctctg ggcggcaagg cccagttcgg
     9241 cggccagcgc ttcggggaga tggaggtgtg ggccctcgag gcctacgggg ccgcccacac
     9301 cctgcaggag atgctgacca tcaagtccga cgacattgag ggcaggaacg ccgcctacga
     9361 ggccatcatc aagggcgagg acgtgcccga gcccagcgtg cccgagtcct tccgcgtgct
     9421 ggtgaaggag cttcaggccc tggccctgga cgtgcagacc ctggacgaga aggacaaccc
     9481 cgtggacatc tttgaggggt tggcctccaa gcggtgaatt cggatcccgg tgatgccggc
     9541 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
     9601 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
     9661 aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
     9721 gcgcggcagc catatgaaaa aggaagtccg caaggtccgc atcgccctgg cctcccccga
     9781 gaagatccgc tcctggagct acggggaggt ggagaagccc gagaccatca actaccgcac
     9841 cctgaagccc gagcgggacg ggctctttga cgagcgcatc ttcggcccca tcaaggacta
     9901 cgagtgcgcc tgcggcaagt acaagcgcca gcgctttgag ggcaaggtct gcgagcggtg
     9961 cggggtggag gtgacgagga gcatcgtccg ccgctaccgc atgggccaca ttgagctggc
    10021 cacccccgcc gcccacatct ggttcgtcaa ggacgtgccc tccaagatcg gcaccctcct
    10081 ggacctctcc gccaccgagc tggagcaggt cctctacttc aacaagtaca tcgtcctgga
    10141 ccccaagggg gccgtcctgg acggggtgcc ggtggaaaag cgccagctcc tcaccgacga
    10201 ggagtaccgg gagctccgct acggcaagca ggagacctac cccctgcccg ccggggtgga
    10261 tgccctggtc aaggacgggg aagaggtggt gaagggccag gagctggccc ccggcgtggt
    10321 gagccgcatg gacggcgtgg ctctctaccg cttcccccgc cgggtgcggg tggactacct
    10381 gaggaaggag cgggccgccc tcaggatccc cctttccgcc tgggtggaga aggaggccta
    10441 ccggcccggg gaggtcctgg cggagctatc tgagccctac ctcttccggg ccgaggagag
    10501 cggggtggtg gagctcaagg atctcgccga gggccacctc atctacctgc gccaggagga
    10561 ggaggtggtg gcccgctact tcctccccgc gggcatgact cccctggtgg tggaggggga
    10621 gatcgtggag gtgggccagc ctctggccga ggggaagggg ctattgcgcc tgccccgcca
    10681 catgacggcc aaggaggtgg aggccgagga ggaaggggac agcgtccacc tcaccctgtt
    10741 cctggagtgg accgagccca aggactacaa ggtggccccc cacatgaacg tcatcgtccc
    10801 cgagggggcc aaggtgcagg cgggggagaa gatcgtggcc gccattgacc ccgaggagga
    10861 ggtcatcgcc gaggccgagg gggtggtcca cctgcacgag cccgccagca tcctggtggt
    10921 caaggcccgg gtctacccct ttgaggacga cgtggaggtg accaccgggg accgggtggc
    10981 ccccggggac gtcctggccg acgggggcaa ggtcaagagc gagatctacg gccgggtgga
    11041 ggtggacctg gtccgcaatg tggtgcgggt ggtggagtcc tacgacatag acgcccgcat
    11101 gggggccgag gccatccagg agctcctcaa ggagctggac ctggagaagc tggagaggga
    11161 gcttttggag gagatgaagc acccctcccg ggcccggcgg gccaaggcca ggaagcggct
    11221 ggaggtggtg cgggccttcc tggactccgg caaccgcccg gagtggatga tcctggaggc
    11281 cgtccccgtc ctgcccccgg acctccgccc catggtccag gtggacgggg ggcggttcgc
    11341 caccagcgac ctcaacgacc tctaccgccg cctcatcaac cgcaacaacc gcttgaagaa
    11401 gctcctggcc cagggggccc ccgagatcat catccgcaac gagaagcgca tgctccagga
    11461 ggcggtggac gccgtcatag acaacggccg ccggggcagc cccgtcacca accccggctc
    11521 cgagcgcccc ctccgcagcc tcaccgacat cctctccggc aagcagggcc gcttccgcca
    11581 gaaccttctg ggcaaacggg tggactactc gggccggagc gtgatcgtgg tggggcccca
    11641 gctcaagctc caccagtgcg gcctgcccaa gcgcatggcc ctggagctct tcaagccctt
    11701 cctcctcaag aagatggagg agaagggcat cgcccccaac gtgaaggcgg ccaggcgcat
    11761 gctggagcgc cagcgggaca tcaaggacga ggtgtgggac gccctcgagg aggtcatcca
    11821 cggcaaggtg gtcctcctga accgggcccc taccctgcat cgcctaggga tccaggcctt
    11881 ccagcccgtt ttggtggagg ggcagtccat ccagctccac cccctggtct gcgaggcctt
    11941 caacgccgac tttgacgggg accagatggc cgtccacgtc cccctttcct ccttcgccca
    12001 ggccgaggcc cggatccaga tgctctccgc ccacaacctt ctctccccgg cctcggggga
    12061 gcccctggcc aagcccagcc gggacatcat cctgggcctt tactacatca cccaggtgcg
    12121 aaaggagaag aagggagccg gcatggcctt cgctaccccc gaggaggctt tggcggctta
    12181 cgaaaggggc gaggtggccc tgaacgcccc catcgtggtg gcgggcaggg agaccagcgt
    12241 gggccgcttg aagttcgtct tcgccaaccc cgacgaggcc cttctggcgg tggcccacgg
    12301 gcttttggac ctccaggacg tggtcaccgt ccgctacctg ggcaggcggc tggagacgag
    12361 cccggggcgg atcctcttcg cccgcatcgt gggcgaggcc gtgggggacg aaaaggtggc
    12421 ccaggagctc atccagatgg acgtccccca ggagaagaac tccctcaagg acctggtcta
    12481 ccaggccttc ctgcgcctgg gcatggagaa gaccgcccgc ctcctggacg ccctcaagta
    12541 ctacggtttc accctctcca ccaccagcgg catcaccatc ggcattgacg atgccgtgat
    12601 ccccgaggag aagcagaggt acctggagga agccgaccgg aagctccgcc agattgagca
    12661 ggcctacgag atgggcttcc tcaccgaccg ggagcggtac gaccaggtga tccagctttg
    12721 gacggagacc acggagaagg tgacccaggc ggtcttcaag aactttgagg agaactaccc
    12781 cttcaacccc ctctacgtca tggcccagtc cggggcccgg ggtaacccgc agcagatccg
    12841 ccagctctgc ggcatgcggg gcctgatgca aaagccctcg ggggagacct ttgaggtgcc
    12901 ggtgcgctcc tccttccgcg agggcctcac cgtcttggag tacttcatca gtagccacgg
    12961 ggccaggaag ggcggggcgg acaccgcgct ccgcaccgcc gactccggct acctgacccg
    13021 gaagctggtg gatgtggccc acgagatcgt ggtgcgggag gcggactgcg gcaccaccaa
    13081 ctacatctcc gtccccctct tccagatgga cgaggtgacc cgcaccctgc gcctcaggaa
    13141 gcgctcggac atagagtccg gcctctacgg ccgggtcctg gcccgggagg tggaggctct
    13201 gggccgcagg ctggaggagg ggcgctacct ctccctcgag gacgtccact tcctcatcaa
    13261 ggcggcggag gcgggggagg tgcgggaggt tcccgtgagg agccccctca cctgccagac
    13321 ccgttacggg gtctgccaga agtgctacgg ctacgacctg tccatggccc gccccgtgtc
    13381 catcggggag gccgtgggcg tagtggccgc cgagtccatc ggcgagcccg gcacccagct
    13441 caccatgcgc accttccaca ccgggggcgt ggcggtgggc accgacatca cccagggtct
    13501 tccccgggtc attgagctct ttgaggcccg ccgccccaag gccaaggcgg tcatctccga
    13561 gattgacggc gtggtgcgca ttgaggaagg cgaggaccgg ctctcggtct tcgtggagtc
    13621 cgagggcttc tccaaggagt acaagctccc caaggacgcc cgccttttgg tcaaggacgg
    13681 ggactacgtg gaggcgggcc agcccctgac ccgcggggcc attgaccccc accagctttt
    13741 ggaggccaag ggtcccgagg cggtggagcg ctacctggtg gacgagatcc agaaggtcta
    13801 ccgggcccag ggcgtgaagc tccacgacaa gcacattgag atcgtggtgc ggcagatgct
    13861 caagtacgtg gaggtcaccg accccgggga tagccgcctt ttggagggcc aggtcctgga
    13921 gaagtgggac gtggaggccc tgaacgagcg cctgatcgcc gagggcaagg tgcccgtggc
    13981 ctggaagccc ctcctcatgg gggtcaccaa gagcgccctc tccaccaaga gctggctctc
    14041 cgccgccagc ttccagaaca ccacccacgt cctcactgag gcggccatcg ccggcaagaa
    14101 ggacgagctc atcggcctca aggagaacgt gatcctgggt cgcctgatcc ctgcgggcac
    14161 gggctccgac ttcgtccgct tcacccaggt ggtggaccag aggaccctga aggccattga
    14221 ggaggcccgc aaggaggcgg tggaggccaa ggagaaggag gctccccgcc gccccgtgcg
    14281 ccgggagcag ccggggaagg gcctttgaat tgatctcgat cccgcgaaat taatacgact
    14341 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
    14401 taagaaggag atataccatg gcggaaccgg gcattgacaa gctcttcggc atggtggact
    14461 ccaagtaccg gctcacggtg gtggtggcca aacgcgccca gcagcttctg cgccaccgct
    14521 ttaagaacac ggttctggag ccggaggaga ggcccaagat gcgcaccctc gagggcctct
    14581 acgacgaccc caacgccgtt acctgggcca tgaaggagct tttgacgggg cggcttttct
    14641 tcggggagaa cctggtgccc gaggaccggc ttcagaagga gatggaaagg ctctacccca
    14701 ccgaagagga ggcctagtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
    14761 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
    14821 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
    14881 aggaggaact atatccggat
//
