LOCUS       sequence-429538-        3008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, 
            includes linker for C-terminal tagging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Modaffari D, Finlayson A, Miao Y, Wallace EWJ, Sawin KE
  TITLE     Improved gene editing and fluorescent-protein tagging in Aspergillus
            nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system.
  JOURNAL   Wellcome Open Res. 2024 Oct 17;9:602. doi: 
            10.12688/wellcomeopenres.23086.1. eCollection 2024.
  PUBMED    39640368
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Wellcome 
            Open Res."; date: "2024-10-17"; volume: "9:602. doi"; pages: " 
            10.12688/wellcomeopenres.23086.1. eCollection 2024"
COMMENT     Sequence Label: pDM042
FEATURES             Location/Qualifiers
     source          1..3008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             291..977
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(1018..1035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1189..1777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1269..1288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1948..2739,2740..2808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2571..2590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2809..2913)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2981..2999
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc caggctgctg ctctcgagct cgtcgaccct atggactcca
      301 ccgaggccgt catcaaggag tttatgcgtt ttaaggttca catggaaggt tctatgaacg
      361 gtcacgagtt cgagatcgag ggtgagggtg agggtcgccc ctacgagggt acccagaccg
      421 ccaagttacg tgttactaaa ggtggtcctt tacctttctc ctgggacatc ctctctcctc
      481 agttcatgta cggttcccgc gccttcacca agcaccccgc tgatattccc gattattgga
      541 agcagtcctt tcccgagggt ttcaagtggg agcgcgtcat gaacttcgaa gatggtggtg
      601 ccgtctctgt tgcccaagat acttcccttg aggacggtac cctcatctac aaggtcaagc
      661 tccgcggtac caattttcct cctgatggtc ccgtcatgca gaagaagacc atgggttggg
      721 aggcctccac cgaacgtctc taccctgagg atgtcgttct taaaggtgac atcaagatgg
      781 cccttcgcct taaggacggt ggtcgctacc tcgctgactt caagaccacc taccgtgcta
      841 aaaagcccgt tcagatgcct ggtgccttca acatcgaccg caagctcgac atcacctccc
      901 acaacgagga ctacaccgtc gtcgaacaat atgagcgttc cgttgctcgt cattctaccg
      961 gtggttctgg tggttcctaa ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1021 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1081 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1141 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1201 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1261 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1321 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1381 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1441 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1501 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1561 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1621 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1681 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1741 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1801 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1861 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1921 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1981 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2041 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2101 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2161 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2221 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2281 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2341 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2401 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2461 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2521 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2581 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2641 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2701 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2761 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2821 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2881 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2941 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3001 ctttcgtc
//
