LOCUS       sequence-429628-        2656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO2te
FEATURES             Location/Qualifiers
     source          1..2656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..583
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     484..503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     700..717
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     850..866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     850..866
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1189..1206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1189..1205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1326..2531
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     primer_bind     2532..2551
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2532..2550
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(2639..2656,1))
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tctagaaata ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg
       61 taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct
      121 gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg
      181 tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta
      241 ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
      361 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa
      421 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc
      481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
      541 tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc
      601 cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg
      661 taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa
      721 gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg
      781 ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatataca
      841 ctccgctagc aggaaacagc tatgaccatg cccgggtaag tgactagcgg aaacacagaa
      901 aaaagcccgc acctgacagt gcgggctttt tttttcgacc aaaggctccg aagagccgag
      961 tgtcttcggt tcgagaccga attcgcgagc tctcgctagc cagtttaaac aattgcggcc
     1021 gcgtactagt ctatcgattt ggatccaccc tgcaggaagt ctagaccgtc gacacggtct
     1081 ctgagagaag acacgtcgct cttcgacgta agtaactgac tcggtaccaa attccagaaa
     1141 agaggcctcc cgaaaggggg gccttttttc gttttggtcc taagcttaac tggccgtcgt
     1201 tttacatacg gccccaaggt ccaaacggtg atagggataa cagggtaatg taccatttac
     1261 gttgacacca cctttcgcgt atggcatgac agcgcccgga agagagtcaa ttcagggtgg
     1321 tgaatatgaa tagttcgaca aagatcgcat tggtaattac gttgctcgat gccatgggga
     1381 ttggccttat catgccagtc ttgccaacgt tattacgtga atttattgct tcggaagata
     1441 tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt aatgcaggtt atctttgctc
     1501 cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc agtgctgttg ttgtcattaa
     1561 taggcgcatc gctggattac ttattgctgg ctttttcaag tgcgctttgg atgctgtatt
     1621 taggccgttt gctttcaggg atcacaggag ctactggggc tgtcgcggca tcggtcattg
     1681 ccgataccac ctcagcttct caacgcgtga agtggttcgg ttggttaggg gcaagttttg
     1741 ggcttggttt aatagcgggg cctattattg gtggttttgc aggagagatt tcaccgcata
     1801 gtcccttttt tatcgctgcg ttgctaaata ttgtcacttt ccttgtggtt atgttttggt
     1861 tccgtgaaac caaaaataca cgtgataata cagataccga agtaggggtt gagacgcaat
     1921 caaattcggt gtacatcact ttatttaaaa cgatgcccat tttgttgatt atttattttt
     1981 cagcgcaatt gataggccaa attcccgcaa cggtgtgggt gctatttacc gaaaatcgtt
     2041 ttggatggaa tagcatgatg gttggctttt cattagcggg tcttggtctt ttacactcag
     2101 tattccaagc ctttgtggca ggaagaatag ccactaaatg gggcgaaaaa acggcagtac
     2161 tgctcggatt tattgcagat agtagtgcat ttgccttttt agcgtttata tctgaaggtt
     2221 ggttagtttt ccctgtttta attttattgg ctggtggtgg gatcgcttta cctgcattac
     2281 agggagtgat gtctatccaa acaaagagtc atcagcaagg tgctttacag ggattattgg
     2341 tgagccttac caatgcaacc ggtgttattg gcccattact gtttgctgtt atttataatc
     2401 attcactacc aatttgggat ggctggattt ggattattgg tttagcgttt tactgtatta
     2461 ttatcctgct atcaatgacc ttcatgttga cccctcaagc tcaggggagt aaacaggaga
     2521 caagtgctta gtaatacgac tcactatagg gtaactaatt gaggcgcgcc taccaccgtc
     2581 aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa ccttcctagg taagtgaccc
     2641 tttagtgagg gttaat
//
