LOCUS       sequence-42964-e        4570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE4k-RFP
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1857..2534
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      2567..2638
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2654..2681
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2839..3427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2919..2938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3515..3609)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3640..4434)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3751..3770)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4361..4380
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtctcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc
       61 cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg
      121 agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagctgca aaaattgcgg
      181 cgttaaatcc ggcgtcggtt ccacacttaa aaatagcgcc agtcgctcca tcatattgcg
      241 cagttcacga atattgcccg gccagtcgta gtgcaccagc acggtttcgc ttgcctgtaa
      301 tccctggcgt aatgcggcag aaaacggggt ggagagcgcc gccagagaca ctttcaaaaa
      361 gctttccgcc agtggcagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg
      421 caaaatactc agccgataaa acaggtcacg gcgaaactgc ccttgccgca tatcttcttc
      481 cagattgcag tgagtggcgc taatgacccg cacatccacc ggaacaggct gatgcccgcc
      541 gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca acggcagcgg
      601 catttcgcca atctcatcga gaaacagcgt acctccgtgg gcgatttcaa acagcccggc
      661 gcgaccgccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc
      721 cagcagcgat tcggcaatcg ccccgcagtt gactgcaaca aacggatgcg actttttgcc
      781 ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt
      841 gcccgtcccc gtttccccct caatcaacac cgctgcactg gagcgggcat acagcaaaat
      901 agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg
      961 agttctcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat
     1021 atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt
     1081 cattccggct tcttctgcca gatcggtaat cagccccgcg ccgaccaccg cttcggtgcc
     1141 gttagctttt agctcgttaa tctgcccgcg tgcgtcttct tcggtaatgt agctacgttg
     1201 atcgaggcgc aaattaaagg ttttttgaaa cgccaccagt gccggaatag tttcctgata
     1261 agtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc
     1321 gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc
     1381 gttagatcca gcggcgatga tggcgtcaca gcgttcgttt gccagtttct tgtggatgta
     1441 ggtcaccgct ttttcaaagc caagctggat aggggtaatg ttcgccaggt gatcaaactc
     1501 aaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg
     1561 tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa
     1621 tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa
     1681 acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa
     1741 ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg
     1801 caaataacaa gttgataaca agaattcaaa agatctttta agaaggagat atacatatgg
     1861 cgagtagcga agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg
     1921 ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc
     1981 agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt
     2041 ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact
     2101 acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg
     2161 gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag
     2221 ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg
     2281 gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca
     2341 aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca
     2401 tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca
     2461 cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact
     2521 ccaccggtgc ttaaggatcc aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa
     2581 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2641 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacct agggcgttcg
     2701 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2761 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3001 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3241 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3481 acgttaaggg attttggtca tgactagtgc ttggattctc accaataaaa aacgcccggc
     3541 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     3601 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     3661 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3721 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3781 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3841 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     3901 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     3961 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     4021 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     4081 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     4141 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     4201 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     4261 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     4321 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     4381 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     4441 cgatcctcat cctgtctctt gatcagatca tgatcccctg cgccatcaga tccttggcgg
     4501 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     4561 tggcaattcc
//
