LOCUS       sequence-42965-e        4616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA4a-RFP
FEATURES             Location/Qualifiers
     source          1..4616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1857..2534
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      2567..2638
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2654..2681
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2709..2726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2843..3388)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2923..2942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3502..3596)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3626..4417,4418..4486))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4249..4268
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4487..4591)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtctcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc
       61 cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg
      121 agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagctgca aaaattgcgg
      181 cgttaaatcc ggcgtcggtt ccacacttaa aaatagcgcc agtcgctcca tcatattgcg
      241 cagttcacga atattgcccg gccagtcgta gtgcaccagc acggtttcgc ttgcctgtaa
      301 tccctggcgt aatgcggcag aaaacggggt ggagagcgcc gccagagaca ctttcaaaaa
      361 gctttccgcc agtggcagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg
      421 caaaatactc agccgataaa acaggtcacg gcgaaactgc ccttgccgca tatcttcttc
      481 cagattgcag tgagtggcgc taatgacccg cacatccacc ggaacaggct gatgcccgcc
      541 gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca acggcagcgg
      601 catttcgcca atctcatcga gaaacagcgt acctccgtgg gcgatttcaa acagcccggc
      661 gcgaccgccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc
      721 cagcagcgat tcggcaatcg ccccgcagtt gactgcaaca aacggatgcg actttttgcc
      781 ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt
      841 gcccgtcccc gtttccccct caatcaacac cgctgcactg gagcgggcat acagcaaaat
      901 agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg
      961 agttctcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat
     1021 atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt
     1081 cattccggct tcttctgcca gatcggtaat cagccccgcg ccgaccaccg cttcggtgcc
     1141 gttagctttt agctcgttaa tctgcccgcg tgcgtcttct tcggtaatgt agctacgttg
     1201 atcgaggcgc aaattaaagg ttttttgaaa cgccaccagt gccggaatag tttcctgata
     1261 agtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc
     1321 gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc
     1381 gttagatcca gcggcgatga tggcgtcaca gcgttcgttt gccagtttct tgtggatgta
     1441 ggtcaccgct ttttcaaagc caagctggat aggggtaatg ttcgccaggt gatcaaactc
     1501 aaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg
     1561 tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa
     1621 tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa
     1681 acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa
     1741 ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg
     1801 caaataacaa gttgataaca agaattcaaa agatctttta agaaggagat atacatatgg
     1861 cgagtagcga agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg
     1921 ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc
     1981 agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt
     2041 ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact
     2101 acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg
     2161 gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag
     2221 ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg
     2281 gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca
     2341 aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca
     2401 tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca
     2461 cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact
     2521 ccaccggtgc ttaaggatcc aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa
     2581 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2641 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacct agggatatat
     2701 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     2761 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     2821 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     2881 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2941 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     3001 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     3061 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     3121 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     3181 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     3241 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     3301 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     3361 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt
     3421 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata
     3481 taagttgtta ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     3541 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     3601 cgagctcgta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3661 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3721 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3781 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3841 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3901 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3961 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4021 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4081 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4141 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4201 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4261 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4321 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4381 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4441 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4501 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4561 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacct
//
