LOCUS       sequence-429653-        9038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing epegRNAs for the induction of EGFR 
            L858R and T790M mutations, through tandem U6 expression of the two 
            epegRNAs using independent U6 promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-EGFR-L858R-T790M-dual-epegRNA
FEATURES             Location/Qualifiers
     source          1..9038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        365..469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(470..538,539..1330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(688..707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1501..2089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1990..2009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2243..2260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2377..2398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2413..2418,2419..2436,2437..2443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2451..2467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2456..2478
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2475..2491
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2475..2491
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2510..2530
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2512..2530
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2558..2784
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2785..2965
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3012..3137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3630..3863
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4048..4092
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4270..4518
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4540..4615
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4801..5049
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        5071..5146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5386..5503
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5642..6820
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5873..6811
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6768..6788
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6833..7432
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6833..6852)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7329..7349
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7448..8036
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7501..7521)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7919..7930)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8108..8341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8419..8540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8456..8475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8510..8529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8580..8715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8735..8754)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8736..8754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8764..8781)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8764..8780)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8773..8795)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8922..9038,1..339)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9009..9028)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
       61 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      121 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      181 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      241 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      301 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
      361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
      781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
      841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
      901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
      961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1621 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     2281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     2341 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     2401 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     2461 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     2521 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     2581 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     2641 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     2701 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     2761 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     2821 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     2881 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     2941 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     3001 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     3061 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     3121 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     3181 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     3241 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     3301 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     3361 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     3421 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     3481 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     3541 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     3601 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     3661 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     3721 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     3781 ttgcaactca cagtctgggg cataaagcag ctccaggcaa gaatcctggc tgtggaaaga
     3841 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     3901 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     3961 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     4021 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     4081 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     4141 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     4201 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     4261 ggacccagag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     4321 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     4381 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     4441 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     4501 tggaaaggac gaaacaccgc aagatcacag attttgggcg ttttagagct agaaatagca
     4561 agttaaaata aggctagtcc gttatcaact tgaaaaagtg ggaccgagtc ggtccagttt
     4621 ggcccgccca aaatctgtga tcttcaggag ccccccccct gaacccagga taaccctcaa
     4681 agtcgggggg ctttttttaa gcttgggccg ctcgaggtac ctctctacat atgacatgtg
     4741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgttggatcc
     4801 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     4861 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     4921 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     4981 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     5041 cgaaacaccg tagtccagga ggcagccgaa gttttagagc tagaaatagc aagttaaaat
     5101 aaggctagtc cgttatcaac ttgaaaaagt gggaccgagt cggtccatca tgcagctcat
     5161 gcccttcggc tgcctcctgt caggagcccc ccccctgaac ccaggataac cctcaaagtc
     5221 ggggggcttt ttttaagctt gggccgctcg aggtacctct ctacatatga catgtgagca
     5281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt ggatccaagc
     5341 ttggcgtaac tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     5401 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     5461 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     5521 cagcagagat ccactttggc gccggctcga gggggcccgg gtgcaaagat ggataaagtt
     5581 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     5641 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     5701 gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     5761 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     5821 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     5881 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     5941 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     6001 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     6061 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     6121 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     6181 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     6241 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     6301 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     6361 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     6421 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     6481 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     6541 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     6601 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     6661 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     6721 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     6781 agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacggcca ccatgaccga
     6841 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6901 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6961 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7021 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     7081 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7141 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7201 gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7261 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7321 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7381 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta
     7441 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7501 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7561 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7621 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7681 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7741 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7801 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7861 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7921 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7981 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     8041 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8101 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     8161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8341 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8401 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8521 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8581 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8641 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8701 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     8761 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     8821 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     8881 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     8941 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9001 agcgcccgct cctttcgctt tcttcccttc ctttctcg
//
