LOCUS       sequence-429655-        8501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing epegRNA to induce KRAS G13A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-KRAS-G13A-tmpknot-epegRNA
FEATURES             Location/Qualifiers
     source          1..8501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(165..176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             354..587
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    665..786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(702..721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     756..775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      826..961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(981..1000)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(982..1000)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1010..1027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1010..1026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1019..1041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1168..1623
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1255..1274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1465..1486
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1649..1753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1754..1822,1823..2614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1972..1991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2785..3373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3274..3293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3527..3544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3661..3682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3697..3702,3703..3720,3721..3727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3735..3751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3740..3762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3759..3775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3759..3775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3794..3814
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3796..3814
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3842..4068
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4069..4249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4296..4421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4914..5147
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5332..5376
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5554..5802
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5554..5574
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5725..5744
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5824..5899
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    6133..6250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6389..7567
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6620..7558
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7515..7535
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7580..8179
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7580..7599)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8076..8096
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(8195..8501,1..282)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8248..8268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
       61 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
      121 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
      181 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
      241 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
      301 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      361 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
      421 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      481 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      541 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
      601 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
      661 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      721 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      781 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
      841 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      901 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      961 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     1021 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     1081 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1141 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1201 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1261 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1321 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1381 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1441 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1501 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1561 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1621 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1681 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1741 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1801 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1861 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1921 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1981 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     2041 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     2101 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2161 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2221 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2281 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2341 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2401 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2461 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2521 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2581 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2641 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2701 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2761 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2821 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2881 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2941 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3001 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3061 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3121 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3181 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3241 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3301 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3361 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3421 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3481 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3541 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3601 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3661 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3721 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     3781 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     3841 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     3901 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     3961 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     4021 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     4081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     4261 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     4321 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     4381 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4441 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4501 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     4561 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     4621 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     4681 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     4741 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     4801 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     4861 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     4921 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     4981 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     5041 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     5101 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     5161 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     5221 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     5281 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     5341 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     5401 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     5461 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     5521 cagacccacc tcccaacccc gaggggaccc agagagggcc tatttcccat gattccttca
     5581 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
     5641 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     5701 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     5761 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgcttgtgg tagttggagc
     5821 tgggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     5881 agtgggaccg agtcggtcct gcctacgtca ccagctccaa ctaccatcag gagccccccc
     5941 cctgaaccca ggataaccct caaagtcggg gggctttttt taagcttggg ccgctcgagg
     6001 tacctctcta catatgacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6061 gccgcgttgc tggcgttgga tccaagcttg gcgtaactag atcttgagac aaatggcagt
     6121 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6181 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6241 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     6301 ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     6361 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     6421 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     6481 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     6541 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     6601 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     6661 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     6721 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     6781 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     6841 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     6901 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     6961 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     7021 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     7081 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     7141 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     7201 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     7261 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     7321 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     7381 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     7441 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     7501 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     7561 gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     7621 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     7681 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     7741 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     7801 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     7861 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     7921 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca
     7981 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8041 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8101 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8161 ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     8221 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8281 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8341 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8401 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8461 gctcctttcc gggactttcg ctttccccct ccctattgcc a
//
