LOCUS       sequence-429656-        9200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest.
            The cloning site is BsmbI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, 
            Zhou T
  TITLE     Robust and inducible genome editing via an all-in-one prime editor 
            in human pluripotent stem cells.
  JOURNAL   Nat Commun. 2024 Dec 30;15(1):10824. doi: 
            10.1038/s41467-024-55104-1.
  PUBMED    39737975
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-30"; volume: "15(1):10824. doi"; pages: " 
            10.1038/s41467-024-55104-1"
COMMENT     Sequence Label: lenti-tmpknot-epegRNA-puro backbone
FEATURES             Location/Qualifiers
     source          1..9200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    586..711
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             758..938
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(939..1165)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1193..1213)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1193..1211)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1232..1248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1232..1248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1245..1267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1256..1272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1280..1286,1287..1304,1305..1310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1325..1346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1463..1480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1634..2222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1714..1733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2393..3184,3185..3253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3016..3035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3254..3358)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3384..3839)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3521..3542)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3733..3752
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3966..3988
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3980..3997
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3981..3997
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4007..4026
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4007..4025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4046..4181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4221..4342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4232..4251)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4286..4305
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4420..4653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4725..5313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4831..4842
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5240..5260
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5329..5928)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5412..5432)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5909..5928
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5941..7119)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5950..6888
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5973..5993)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7258..7375
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    7590..7608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7615..7633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             7651..7662
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7669..8346
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      8363..8394
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        complement(8405..8653)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8463..8482)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8633..8653)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8831..8875)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9060..9200,1..93)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
       61 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
      121 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
      181 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
      241 agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa
      301 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
      361 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
      421 tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag
      481 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
      541 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
      601 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
      661 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
      721 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
      781 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
      841 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
      901 agctcccagg ctcagatctg gtctaaccag agagacccgt ttatgtatcg agctaggcac
      961 ttaaatacaa tatctctgca atgcggcaat tcagtggttc gtccaatcca tgtcagaccc
     1021 gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca
     1081 cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac
     1141 tacaagagta ttgcataaga ctacattaag cttgcagctc cagcttttgt tccctttagt
     1201 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1261 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1381 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1441 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3361 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     3421 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3481 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3541 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3601 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3661 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3721 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3781 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3841 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3901 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3961 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     4021 atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat
     4081 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     4141 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     4201 atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4261 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4321 tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt gcaagttata
     4381 aatactgaat aataagatga catgaactac tatacgtact gctagagatt ttccacactg
     4441 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     4501 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     4561 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
     4621 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     4681 taagatctac agctgccttg taagtcattg gtcttaaagt cgacgcgggg aggcggccca
     4741 aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg
     4801 cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga
     4861 cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt
     4921 ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc
     4981 aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga
     5041 aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt
     5101 gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca
     5161 ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg
     5221 cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa
     5281 tctttcacaa attttgtaat ccagaggttg attgtcgact taacgcgttc aggcaccggg
     5341 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     5401 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     5461 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     5521 gccctggtgg tcgggcgaga ctccgacggt ggccaggaac cacgcgggct ccttgggccg
     5581 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     5641 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     5701 ccagaccgcc acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     5761 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     5821 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     5881 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggccgtacg
     5941 tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa
     6001 ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg
     6061 cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc
     6121 ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc
     6181 ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc ttttcctttg
     6241 tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc ctccattttg agctccctgc
     6301 agcagggccg ggaagcggcc atctttccgc tcacgcaact ggtgccgacc gggccagcct
     6361 tgccgcccag ggcggggcga tacacggcgg cgcgaggcca ggcaccagag caggccggcc
     6421 agcttgagac tacccccgtc cgattctcgg tggccgcgct cgcaggcccc gcctcgccga
     6481 acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc
     6541 agtgtgcaga tcttggcccg catttacaag actatcttgc cagaaaaaaa gcgtcgcagc
     6601 aggtcatcaa aaattttaaa tggctagaga cttatcgaaa gcagcgagac aggcgcgaag
     6661 gtgccaccag attcgcacgc ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg
     6721 aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc caacccgaag
     6781 ctcgggatca agaatcacgt actgcagcca ggtggaagta attcaaggca cgcaagggcc
     6841 ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct gtgttctggc
     6901 ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc
     6961 cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg
     7021 ccaccttctc taggcaccgg atcaattgcc gacccctccc cccaacttct cggggactgt
     7081 gggcgatgtg cgctctgccc actgacgggc accggagcca attcccactc ctttcaagac
     7141 ctagaaggtc cattagctgc aaagattcct ctctgtttaa aactttatcc atctttgcac
     7201 ccgggccccc tcgagccggc gccaaagtgg atctctgctg tccctgtaat aaacccgaaa
     7261 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     7321 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     7381 gatgaatact gccatttgtc tcaagatcta gttacgccaa gcttggatcc aacgccagca
     7441 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tcatatgtag
     7501 agaggtacct cgagcggccc aagcttaaaa aaagcccccc gactttgagg gttatcctgg
     7561 gttcaggggg ggggctcctg acgagacgct ccctatcagt gatagagatt gacatcccta
     7621 tcagtgatag agatactgag cacggatctg aaagaggaga aaggatctat ggcgagtagc
     7681 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
     7741 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
     7801 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
     7861 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
     7921 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
     7981 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
     8041 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
     8101 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
     8161 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
     8221 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
     8281 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
     8341 gcttaatggc actcctcagc caagtcaaaa gcctccgacc ggaggctttt gactacgcgt
     8401 ctcgggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag
     8461 ttacggtaag catatgatag tccattttaa aacataattt taaaactgca aactacccaa
     8521 gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat
     8581 taattctaat tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg
     8641 gaaataggcc ctctctgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
     8701 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     8761 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     8821 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     8881 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     8941 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
     9001 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
     9061 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
     9121 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     9181 tctgctgctg cactatacca
//
