LOCUS       sequence-429681-        4400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang 
            R, Bishop JM
  TITLE     Distinct pathways of genomic progression to benign and malignant 
            tumors of the liver.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep
            4.
  PUBMED    17785413
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-09-11"; volume: "104(37)"; pages: 
            "14771-6. Epub 2007 Sep 4"
COMMENT     Sequence Label: pENTR-N90-beta-catenin
FEATURES             Location/Qualifiers
     source          1..4400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             488..517
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(2629..2728)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2840..2859)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2851..3660
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2918..2937)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3750..4338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4239..4258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 caccatggag cagaagctga tctccgagga ggacctgaag gatcctcgag ctcagagggt
      541 acgagctgct atgttccctg agacattaga tgagggcatg cagatcccat ctacacagtt
      601 tgatgctgct catcccacta atgtccagcg tttggctgaa ccatcacaga tgctgaaaca
      661 tgcagttgta aacttgatta actatcaaga tgatgcagaa cttgccacac gtgcaatccc
      721 tgaactgaca aaactgctaa atgacgagga ccaggtggtg gttaataagg ctgcagttat
      781 ggtccatcag ctttctaaaa aggaagcttc cagacacgct atcatgcgtt ctcctcagat
      841 ggtgtctgct attgtacgta ccatgcagaa tacaaatgat gtagaaacag ctcgttgtac
      901 cgctgggacc ttgcataacc tttcccatca tcgtgagggc ttactggcca tctttaagtc
      961 tggaggcatt cctgccctgg tgaaaatgct tggttcacca gtggattctg tgttgtttta
     1021 tgccattaca actctccaca accttttatt acatcaagaa ggagctaaaa tggcagtgcg
     1081 tttagctggt gggctgcaga aaatggttgc cttgctcaac aaaacaaatg ttaaattctt
     1141 ggctattacg acagactgcc ttcaaatttt agcttatggc aaccaagaaa gcaagctcat
     1201 catactggct agtggtggac cccaagcttt agtaaatata atgaggacct atacttacga
     1261 aaaactactg tggaccacaa gcagagtgct gaaggtgcta tctgtctgct ctagtaataa
     1321 gccggctatt gtagaagctg gtggaatgca agctttagga cttcacctga cagatccaag
     1381 tcaacgtctt gttcagaact gtctttggac tctcaggaat ctttcagatg ctgcaactaa
     1441 acaggaaggg atggaaggtc tccttgggac tcttgttcag cttctgggtt cagatgatat
     1501 aaatgtggtc acctgtgcag ctggaattct ttctaacctc acttgcaata attataagaa
     1561 caagatgatg gtctgccaag tgggtggtat agaggctctt gtgcgtactg tccttcgggc
     1621 tggtgacagg gaagacatca ctgagcctgc catctgtgct cttcgtcatc tgaccagccg
     1681 acaccaagaa gcagagatgg cccagaatgc agttcgcctt cactatggac taccagttgt
     1741 ggttaagctc ttacacccac catcccactg gcctctgata aaggctactg ttggattgat
     1801 tcgaaatctt gccctttgtc ccgcaaatca tgcacctttg cgtgagcagg gtgccattcc
     1861 acgactagtt cagttgcttg ttcgtgcaca tcaggatacc cagcgccgta cgtccatggg
     1921 tgggacacag cagcaatttg tggagggggt ccgcatggaa gaaatagttg aaggttgtac
     1981 cggagccctt cacatcctag ctcgggatgt tcacaaccga attgttatca gaggactaaa
     2041 taccattcca ttgtttgtgc agctgcttta ttctcccatt gaaaacatcc aaagagtagc
     2101 tgcaggggtc ctctgtgaac ttgctcagga caaggaagct gcagaagcta ttgaagctga
     2161 gggagccaca gctcctctga cagagttact tcactctagg aatgaaggtg tggcgacata
     2221 tgcagctgct gttttgttcc gaatgtctga ggacaagcca caagattaca agaaacggct
     2281 ttcagttgag ctgaccagct ctctcttcag aacagagcca atggcttgga atgagactgc
     2341 tgatcttgga cttgatattg gtgcccaggg agaacccctt ggatatcgcc aggatgatcc
     2401 tagctatcgt tcttttcact ctggtggata tggccaggat gccttgggta tggaccccat
     2461 gatggaacat gagatgggtg gccaccaccc tggtgctgac tatccagttg atgggctgcc
     2521 agatctgggg catgcccagg acctcatgga tgggctgcct ccaggtgaca gcaatcagct
     2581 ggcctggttt gatactgacc tgtaagcggc cgcactcgag atatctagac ccagctttct
     2641 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     2701 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa
     2761 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2821 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2881 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2941 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     3001 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3061 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3121 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     3181 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgtccctgcg ccggttgcat
     3241 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3301 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3361 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3421 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     3481 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3541 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3601 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3661 tcagaattgg ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca
     3721 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3781 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3841 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3901 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3961 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4021 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4081 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4141 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4201 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4261 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4321 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4381 tggccttttg ctcacatgtt
//
