LOCUS       sequence-429688-       11700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible hRTN3E (long form) expression vector, expresses 
            hRTN3E-TEV-Halo, Lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Crapart CC, Scott ZC, Konno T, Sharma A, Parutto P, Bailey DMD, 
            Westrate LM, Avezov E, Koslover EF
  TITLE     Luminal transport through intact endoplasmic reticulum limits the 
            magnitude of localized Ca(2+) signals.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2312172121. doi: 
            10.1073/pnas.2312172121. Epub 2024 Mar 19.
  PUBMED    38502705
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-03-26"; volume: "121(13):e2312172121. 
            doi"; pages: " 10.1073/pnas.2312172121. Epub 2024 Mar 19"
COMMENT     Sequence Label: pLV_TRE3G_hRTN3E-Halo
FEATURES             Location/Qualifiers
     source          1..11700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1964..1982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2000..2018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2036..2054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2072..2090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2180..2198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2243..2267
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2334..2358
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2359..2368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2826..2837)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5461..5481
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5482..6369
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     protein_bind    complement(6373..6397)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6415..7003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6468..6488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6886..6897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7034..7533
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7069..7092)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7466..7485
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7550..8149
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7550..7569)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8046..8066
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8221..8454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8526..8647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8563..8582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8617..8636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8687..8822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8842..8861)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8843..8861)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8871..8888)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8871..8887)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8880..8902)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9029..9484
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9116..9135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9326..9347
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9510..9614
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9615..9683,9684..10475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9833..9852)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10646..11234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11135..11154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11388..11405
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11522..11543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11558..11563,11564..11581,11582..11588)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11596..11612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11601..11623
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11620..11636
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11620..11636
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11655..11675
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11657..11675
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtt tactccctat cagtgataga
     1981 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     2041 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     2101 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     2161 gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta
     2221 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt
     2281 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaacaagtt
     2341 tgtacaaaaa agcaggctgc caccatggcg gagccgtcgg cggccactca gtcccattcc
     2401 atctcctcgt cgtccttcgg agccgagccg tccgcgcccg gcggcggcgg gagcccagga
     2461 gcctgccccg ccctggggac gaagagctgc agctcctcct gtgcggattc ctttgtttct
     2521 tcctcttcct ctcagcctgt atctctattt tcgacctcac aagagggatt gagctctctt
     2581 tgctctgatg agccatcttc agaaattatg acttcttcct ttctttcatc ttctgaaata
     2641 cataacactg gccttacaat actacatgga gaaaaaagcc atgtgttagg gagccagcct
     2701 attttagcca aagaaggaaa agaccacttg gatcttctag atatgaaaaa gatggaaaag
     2761 cctcagggga ccagcaacaa cgtatcagac tcttcagttt ctcttgcagc aggagttcat
     2821 tgtgaccgtc cttctattcc agccagtttc ccagagcatc ctgcttttct ctcaaagaaa
     2881 attggtcaag tggaagagca aatagataaa gagaccaaga acccaaatgg ggtatcaagt
     2941 agggaggcta aaactgcatt ggatgctgat gacagattca ctttgctgac agcccagaaa
     3001 ccacctactg agtactctaa ggtagaaggc atttatacat attctttgtc tccatccaaa
     3061 gtttcaggag atgatgttat tgaaaaggat tcccctgaat caccatttga agtaattatt
     3121 gacaaagcag catttgacaa agaatttaaa gactcatata aggagagcac agatgatttt
     3181 ggtagctggt ctgtgcacac tgataaagaa tcatccgaag acatttcaga gactaatgac
     3241 aagctttttc cactgagaaa taaagaggca ggacgttacc caatgtctgc attgctcagt
     3301 aggcagtttt cacacacaaa tgcagcactg gaagaggtgt ccagatgcgt gaatgatatg
     3361 cataacttta ctaacgaaat actgacttgg gatctggttc cccaagtgaa acaacagacc
     3421 gataaatctt ctgactgcat cacaaaaact acaggacttg acatgagtga atataattca
     3481 gaaattccag ttgtaaatct taaaactagc actcatcaga aaactcctgt atgttctatt
     3541 gatgggagca ctcccatcac taaatcaaca ggtgattggg cagaagcatc tctccagcaa
     3601 gaaaatgcta ttactggaaa acctgtacct gactctttga attccacaaa agaattcagt
     3661 atcaaaggtg tgcaaggcaa tatgcagaaa caggatgaca cacttgcaga attacctgga
     3721 tctccacctg agaaatgtga ctctttgggt tctggagtgg ccacagtgaa agtggtttta
     3781 cctgatgacc acctgaaaga tgaaatggac tggcagagct ctgcattggg agaaatcaca
     3841 gaagctgata gttctggtga gtctgatgac acagtaatag aggacatcac agcagataca
     3901 tcatttgaaa ataacaaaat tcaggctgaa aaacctgttt ccattccaag tgctgttgta
     3961 aaaacaggtg aaagagaaat caaagagatt cccagttgtg agagagaaga aaaaacatct
     4021 aaaaactttg aagaattggt cagtgactct gagctgcatc aagatcagcc tgatattctt
     4081 ggaaggagtc cagctagtga ggcagcatgt tcaaaagtac ccgatacgaa tgtctcctta
     4141 gaagatgtga gtgaagttgc tcctgaaaag cctattacta ctgagaaccc caaacttcct
     4201 tcaacagtgt ctccaaatgt ttttaatgag acagaattct cattaaatgt gacaacatct
     4261 gcctatttgg agtcattaca tgggaaaaat gttaaacata tagatgattc ctccccagag
     4321 gacctgatag cagcctttac agaaaccaga gataaaggaa tagtagatag tgaaagaaat
     4381 gcttttaaag caatatcaga gaagatgaca gactttaaaa caactcctcc tgtagaagtc
     4441 ttacatgaaa atgagtccgg tggttctgaa attaaagaca ttggaagcaa atacagtgaa
     4501 caaagcaaag aaacaaatgg aagtgagcct ctaggtgttt tccctaccca aggtactcca
     4561 gtagcatctc ttgacttaga acaagaacag ctcacaatta aggctcttaa agaattaggt
     4621 gaaagacagg ttgagaagtc aacttctgca cagcgtgacg cagaattgcc ttctgaagaa
     4681 gtactgaagc aaactttcac atttgctcca gaatcttggc cacagagatc atatgacatc
     4741 ctagaacgta atgtcaagaa tggatctgat cttgggattt cccagaagcc catcactatc
     4801 agagaaacta ctagggtaga tgctgtttcc agccttagca agactgaatt ggtaaaaaag
     4861 catgtcctag caagacttct gacagacttc tcagtgcacg atctgatttt ctggagagat
     4921 gtgaagaaga ctgggtttgt ctttggcacc acgctgatca tgctgctttc cctggcagct
     4981 ttcagtgtca tcagtgtggt ttcttacctc atcctggctc ttctctctgt caccatcagc
     5041 ttcaggatct acaagtccgt catccaagct gtacagaagt cagaagaagg ccatccattc
     5101 aaagcctacc tggacgtaga cattactctg tcctcagaag ctttccataa ttacatgaat
     5161 gctgccatgg tgcacatcaa cagggccctg aaactcatta ttcgtctctt tctggtagaa
     5221 gatctggttg actccttgaa gctggctgtc ttcatgtggc tgatgaccta tgttggtgct
     5281 gtttttaacg gaatcaccct tctaattctt gctgaactgc tcattttcag tgtcccgatt
     5341 gtctatgaga agtacaagac ccagattgat cactatgttg gcatcgcccg agatcagacc
     5401 aagtcaattg ttgaaaagat ccaagcaaaa ctccctggaa tcgccaaaaa aaaggcagaa
     5461 gaaaacctgt attttcaggg cgcagaaatc ggtactggct ttccattcga cccccattat
     5521 gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga tggcacccct
     5581 gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat catcccgcat
     5641 gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa atccgacaaa
     5701 ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt catcgaagcc
     5761 ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct gggtttccac
     5821 tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt catccgccct
     5881 atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc cttccgcacc
     5941 accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg tacgctgccg
     6001 atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga gccgttcctg
     6061 aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat cgccggtgag
     6121 ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca gtcccctgtc
     6181 ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga agccgctcgc
     6241 ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct gaatctgctg
     6301 caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc gacgctcgag
     6361 atttccggct aaacccagct ttcttgtaca aagtggtgat aatcgaattc cgataatcaa
     6421 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6481 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6541 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6601 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6661 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6781 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6841 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6901 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6961 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcccgcgg
     7021 ttcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     7081 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     7141 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     7201 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     7261 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     7321 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     7381 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     7441 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     7501 ctgttctcct cttcctcatc tccgggcctt tcgacctcac gtggccacca tgaccgagta
     7561 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     7621 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     7681 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7741 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     7801 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7861 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     7921 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     7981 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8041 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8101 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag gtacctttaa
     8161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8761 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8821 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     8881 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8941 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9001 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9061 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9121 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9181 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9241 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9301 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9361 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9421 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9481 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11041 gagaaagcgc cacgcttccc gaagagagaa aggcggacag gtatccggta agcggcaggg
    11101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11641 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
