LOCUS       sequence-429689-        8923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter-regulated ER targeted HaloTag expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Crapart CC, Scott ZC, Konno T, Sharma A, Parutto P, Bailey DMD, 
            Westrate LM, Avezov E, Koslover EF
  TITLE     Luminal transport through intact endoplasmic reticulum limits the 
            magnitude of localized Ca(2+) signals.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2312172121. doi: 
            10.1073/pnas.2312172121. Epub 2024 Mar 19.
  PUBMED    38502705
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-03-26"; volume: "121(13):e2312172121. 
            doi"; pages: " 10.1073/pnas.2312172121. Epub 2024 Mar 19"
COMMENT     Sequence Label: pLV_CMV_Prss-Halo-KDEL
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1808..1925
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1943..1963
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2024..2327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2328..2531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2482..2502
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2552..2576
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2589..2678
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             2691..3578
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     protein_bind    complement(3600..3624)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3642..4230
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3695..3715)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4113..4124)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4261..4760
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4296..4319)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4693..4712
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4777..5376
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4777..4796)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5273..5293
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5444..5677
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5749..5870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5786..5805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5840..5859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5910..6045
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6065..6084)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6066..6084)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6094..6111)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6094..6110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6103..6125)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6252..6707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6339..6358)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6549..6570
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6733..6837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6838..6906,6907..7698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7056..7075)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7869..8457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8358..8377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8611..8628
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8745..8766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8781..8786,8787..8804,8805..8811)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8819..8835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8824..8846
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8843..8859
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8843..8859
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8878..8898
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8880..8898
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgctg
     1801 aggatccttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1861 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1921 attttactag tgattatcgg atcaactttg tatagaaaag ttgtagttat taatagtaat
     1981 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2041 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2101 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2161 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2221 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2281 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     2341 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     2401 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2461 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2521 taagcagagc tggtttagtg aaccgtcaga tccaagtttg tacaaaaaag caggctgcca
     2581 ccgggcccat ggacagcaaa ggttcgtcgc agaaagggtc ccgcctgctc ctgctgctgg
     2641 tggtgtcaaa tctactcttg tgccagggtg tggtctcctg cagtcgactt gcagaaatcg
     2701 gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg
     2761 tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct
     2821 cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag
     2881 acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg
     2941 tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc
     3001 acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag
     3061 gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg
     3121 cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc
     3181 agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
     3241 agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct
     3301 tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat
     3361 acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg
     3421 ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg
     3481 tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg
     3541 agatcgcgcg ctggctgtcg acgctcgaga tttccggcaa ggacgagctg taactcgaga
     3601 cccagctttc ttgtacaaag tggtgataat cgaattccga taatcaacct ctggattaca
     3661 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3721 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3781 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3841 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3901 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3961 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4021 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     4081 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4141 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4201 gtcggatctc cctttgggcc gcctccccgc atcgggaatt cccgcggttc gaattctacc
     4261 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     4321 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     4381 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     4441 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     4501 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     4561 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     4621 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     4681 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4741 cctcatctcc gggcctttcg acctcacgtg gccaccatga ccgagtacaa gcccacggtg
     4801 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     4861 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     4921 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     4981 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5041 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5101 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     5161 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     5221 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     5281 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5341 cgcacctggt gcatgacccg caagcccggt gcctgagctt taagaccaat gacttacaag
     5401 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5461 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5521 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5581 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5641 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     5701 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     5761 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5821 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5881 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5941 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6001 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6061 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6121 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6181 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6241 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6301 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6361 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6421 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6481 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6541 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6601 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6661 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     6721 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6781 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6841 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6901 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6961 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7021 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7081 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7141 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7201 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7261 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7321 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7381 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7441 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7501 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7561 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7621 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7681 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7741 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7801 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7861 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7921 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7981 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8041 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8101 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8161 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8221 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8281 cccgaagaga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8341 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8401 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8461 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8521 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8581 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8641 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8701 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8761 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8821 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     8881 attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
