LOCUS       sequence-42973-e        4635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE3k-RFP
FEATURES             Location/Qualifiers
     source          1..4635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1922..2599
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      2632..2703
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2719..2746
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2904..3492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2984..3003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3580..3674)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3705..4499)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3816..3835)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4426..4445
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcttaa ttacccgact ggtctttggc accagctttt aaacgacgcc acagagtggt
       61 gcggctaatc cccagataac gcgccgcggc ggtcttatcg cccttaaagc gcgccagtac
      121 atcctgtaac gcgttcgcat ccacggttga aggcgtcagt tctgctgtgt tcaccataag
      181 ctcaggtaat aactgccgca taaattgcct gtccagcgtt ggcgcgggat cgacgcttaa
      241 aaaaagcgcc aggcgctcca tcatattacg cagttcgcga atattaccgg gccagcgcca
      301 ggccagcaaa agcggctgac actgtgtcaa tccatgacgt atcgattcgg taaacggaat
      361 ttccatcgcc gccagcgact gttttaaaaa gctttccgcc agcggcaaaa tatcagcctg
      421 ccgctcgcgc aaaggaggaa gcgtcagacg cagaatactc aggcgataaa agagatcggg
      481 gcgaaaacgt ccttgcatta tctcccgatc cagatcgcaa tgcgtagcgc tgatcacccg
      541 gacatccacc gggatcggct ggtgtccgcc gacgcgagtg acggcttttt cctccagtac
      601 gcgcaaaagt cgggtttgta acggcaaggg catttcgccg atttcatcca gaaacagcgt
      661 gccgccgtgt gcgatttcaa acagccccgc ccggcctcct cgtcgtgaac cggtaaacgc
      721 gccctcttca taaccaaaca gttccgcttc cagcaacgac tcggtaatcg cgccgcaatt
      781 gacggcgaca aagggagggg atggcttatt ctgacggtga ggctggcggt gaaagaacgt
      841 ctggtgaatc gcctgcgccg ccagctcttt tccggtccct gtttccccct gaatcagcac
      901 tgccgcacgg gagcgggcat agagcgtaat cgtctggcgg agctgctcca tttgcggcga
      961 ctggccgcgt atatcgccca gttcataccg ggtttgtaat cccttgccgg atgggtaatc
     1021 cacgcgctgg cgccgtgtca gacgggtcat atccagcgca tcatgaaaag cctgacgaac
     1081 ggtcgccgcg gaataaataa agatggcggt cattcctgcc tcttccgcca gatcggtaat
     1141 taatcccgcg ccgacgacgg cctcaatacc gttggcctta agttcgttaa tttgcccgcg
     1201 cgcgtcctct tcggtgacat agcttcgctg ttcaagacgg aggtgaaacg ttttctgaaa
     1261 ggcaagtaaa gccggaatgg tctcctgata ggtcacgata ccgatagacg aggtgagctt
     1321 tcccgctttc gccagcgcct gtaatacatc gaatccgctg ggcttgatga ggatcaccgg
     1381 tattgacagg cggcttttta aataggcccc attcgaaccc gccgcgataa tcgcgtcgca
     1441 gcgctcggtc gccagttttt tgcgaatgta ggtcaccgcc ttttcaaagc cgagttgaat
     1501 aggcgtgatg gtcgccagat gatcaaattc caggctgata tcccgaaaca gttcgaacag
     1561 acgcgttacg gagaccgtcc agatcaccgg tttatcgcta ttatcgcgcg gagcgctgtg
     1621 ggcagtcgtc atcgcagtag attcatcttt aaggggcgat tttttgtttt aaacgtgttt
     1681 cataaatgtt gcaatgaaac agggtgattc gtttcatgaa acgttagctg acacgttttt
     1741 ttttccctta atcgcgctta ttcataacag aaatgactgt aattacctgt ttttaaatct
     1801 cattgtattt aattttctcg ctgcctcttt gcctggcata gcctttgctt tggtgaatac
     1861 atcttgaata acaatttact aacatggaat tcaaaagatc ttttaagaag gagatataca
     1921 tatggcgagt agcgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     1981 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     2041 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     2101 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     2161 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     2221 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     2281 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     2341 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     2401 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     2461 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     2521 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     2581 tcactccacc ggtgcttaag gatccaaact cgagtaagga tctccaggca tcaaataaaa
     2641 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2701 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctagggc
     2761 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2821 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2881 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2941 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3001 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3061 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3121 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3181 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3241 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3301 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3361 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3421 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3481 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3541 aactcacgtt aagggatttt ggtcatgact agtgcttgga ttctcaccaa taaaaaacgc
     3601 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     3661 tatcaacagg agtccaagcg agctctcgaa ccccagagtc ccgctcagaa gaactcgtca
     3721 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     3781 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     3841 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     3901 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     3961 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     4021 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     4081 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     4141 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     4201 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     4261 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     4321 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     4381 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     4441 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     4501 cgaaacgatc ctcatcctgt ctcttgatca gatcatgatc ccctgcgcca tcagatcctt
     4561 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     4621 ccagctggca attcc
//
