LOCUS       sequence-429737-        9172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor vector for targeting a tdTomato reporter to the human AGER 
            locus at the endogenous ATG start site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, 
            Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, 
            Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, 
            Kotton DN
  TITLE     Generation of human alveolar epithelial type I cells from 
            pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017.
  PUBMED    38642558
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017."
COMMENT     Sequence Label: p2701-AGER-tdTomato
FEATURES             Location/Qualifiers
     source          1..9172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     371..390
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(402..901)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(450..469)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     843..866
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(935..968)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(982..998)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(982..998)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(1718..1736)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1757..1773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1757..1773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1770..1792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1781..1797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1805..1811,1812..1829,1830..1835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1850..1871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1988..2005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2159..2747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2239..2258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2918..3709,3710..3778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3541..3560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3779..3883)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3909..4364)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4046..4067)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4258..4277
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4491..4513
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4505..4522
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4506..4522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4529..4548
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4529..4547
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4567..4583
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6305..7735
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     intron          7786..8015
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7998..8017
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(8061..8078)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8067..8291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8336..8352
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8387..8420)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(8963..9172,1..390))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9046..9066)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc
       61 ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat
      121 ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc
      181 gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc
      241 ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
      301 gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc
      361 gaggcgcacc gtgggcttgt actcggtcat ggtaagctgg tcgaaaggcc cggagatgag
      421 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
      481 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
      541 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
      601 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
      661 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
      721 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
      781 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
      841 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
      901 cggtagaatt aattcctgca ggtcgaggga cctaataact tcgtatagca tacattatac
      961 gaagttatat taagggttcc ggatccacta gttctagact cgagtatggc gcgccgctgg
     1021 tcctcagtct gtggggtgag ccactccctc aaccccactg accctccctg cagaaagcac
     1081 tttaacccca caccccagtc gtcctagaac ttttcccaga acccgaggaa gtgcctttca
     1141 aggtccctca cccaccctgt ccaaattttg ttagccctca ttcccttcct acccctctac
     1201 catggtgcta tctcccaggg gcagtagtag gtgctcaaaa catcacagcc cggattggcg
     1261 agccactggt gctgaagtgt aagggggccc ccaagaaacc accccagcgg ctggaatgga
     1321 aactggtaag cggggctcct gttgcagcct cccaacttcc agggagacca gcaatgattt
     1381 ggatccccgt cactctgcct cacagtcctt tcccaaaggc cttgcactgt ttaggccctg
     1441 cttctctgct tctagaacac aggccggaca gaagcttgga aggtcctgtc tccccaggga
     1501 ggaggcccct gggacagtgt ggctcgtgtc cttcccaaca gctccctctt ccttccggct
     1561 gtcgggatcc aggatgaggg gattttccgg tgccaggcaa tgaacaggaa tggaaaggag
     1621 accaagtcca actaccgagt ccgtgtctac cgtaagaatt ccagggtctt ctccaaggcc
     1681 tgcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaatttcga
     1741 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1801 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1861 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2581 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3901 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3961 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4021 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4081 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4141 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4201 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4261 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4321 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     4381 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     4441 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     4501 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattgggccc
     4561 ctcgatcgag gtcgacggta tcgataagct tctgatggaa ttagaacttg gcaaaacaat
     4621 actgagaatg aagtgtatgt ggaacagagg ctgctgatct cgttcttcag gctatgaaac
     4681 tgacacattt ggaaaccaca gtacttagaa ccacaaagtg ggaatcaaga gaaaaacaat
     4741 gatcccacga gagatctata gatctataga tcatgagtgg gaggaatgag ctggccctta
     4801 atttggtttt gcttgtttaa attatgatat ccaactatga aacattatca taaagcaata
     4861 gtaaagagcc ttcagtaaag agcaggcatt tatctaatcc caccccaccc ccacccccgt
     4921 agctccaatc cttccattca aaatgtaggt actctgttct cacccttctt aacaaagtat
     4981 gacaggaaaa acttccattt tagtggacat ctttattgtt taatagatca tcaatttctg
     5041 caggtcgact ctagctagag gatccccaaa tctctgtagg tagtttgtcc aattatgtca
     5101 caccacagaa gtaaggttcc ttcacaaaga gatcgcctga cacgatttcc tgcacaggct
     5161 tgagccatat actcatacat cgcatcttgg ccacgttttc cacgggtttc aaaattaatc
     5221 tcaagttcta cgcttaacgc tttcgcctgt tcccagttat taatatattc aacgctagaa
     5281 ctcccctcag cgaagggaag gctgagcact acacgcgaag caccatcacc gaaccttttg
     5341 ataaactctt ccgttccgac ttgctccatc aacggttcag tgagacttaa acctaactct
     5401 ttcttaatag tttcggcatt atccactttt agtgcgagaa ccttcgtcag tcctggatac
     5461 gtcactttga ccacgcctcc agcttttcca gagagcgggt tttcattatc tacagagtat
     5521 cccgcagcgt cgtatttatt gtcggtacta taaaaccctt tccaatcatc gtcataattt
     5581 ccttgtgtac cagattttgg cttttgtaaa ttccagcctg caattgatcc gggcaggact
     5641 ggggacaggt tggaagtgtg atgggtgggg gggtgggagg catgggccgg gggcagttct
     5701 ctcctcactt gtaaacttgt gtagtttcac agaaaaaaaa caaaatgcag ttttaaataa
     5761 agaaatttct tttttccctg ggtttagttg agaatttttt tcaaaaaaca tgagaaaccc
     5821 cagaaaaaaa atgattttct ttcacgaagt tccaaacagg tttctctcct gttccccagc
     5881 cttgccttca tgatgcaggc ccaattgcac ccttgcagac aacagtctgg cctgaaccct
     5941 attgatgcaa ctttgcgcaa tcaagatggg gctccagtgg gtcaccaggc agccctgatg
     6001 gactgatgga ataaatagga tcgggggctc tgagggaatg agaccctaga gggtacactc
     6061 cccatccccc agggaagtga ctgtacccag aggctggtag tacccagggg tggggtgata
     6121 attatttctc tagtacctga aggactcttg tcccaaaggc atgaattcct agcattccct
     6181 gtgacaagac gactgaaaga tgggggctgg agagagggtg caggcatcac ctagggcgga
     6241 ggccacagca gggagagggg cagacagagc caggaccctg gaaggaagca ggacgcgtgg
     6301 taccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
     6361 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6421 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     6481 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     6541 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6601 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     6661 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     6721 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     6781 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     6841 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     6901 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     6961 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     7021 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
     7081 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     7141 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     7201 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     7261 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     7321 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     7381 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     7441 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     7501 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     7561 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     7621 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     7681 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaacatat
     7741 gtctagatgg aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct
     7801 caaaagcggg catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga
     7861 tattcacctg gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga
     7921 caatcttttt gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag
     7981 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
     8041 ggtcggagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8101 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8161 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8221 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8281 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgatcctct agactcgagg
     8341 tcgacggtat cgataagcta gcttgggctg caggtcgagg gacctaataa cttcgtatag
     8401 catacattat acgaagttat attaagggtt ccggatcagc ttgatatcga attagcttct
     8461 gatggaatta gaacttggca aaacaatact gagaatgaag tgtatgtgga acagaggctg
     8521 ctgatctcgt tcttcaggct atgaaactga cacatttgga aaccacagta cttagaacca
     8581 caaagtggga atcaagagaa aaacaatgat cccacgagag atctatagat ctatagatca
     8641 tgagtgggag gaatgagctg gcccttaatt tggttttgct tgtttaaatt atgatatcca
     8701 actatgaaac attatcataa agcaatagta aagagccttc agtaaagagc aggcatttat
     8761 ctaatcccac cccaccccca cccccgtagc tccaatcctt ccattcaaaa tgtaggtact
     8821 ctgttctcac ccttcttaac aaagtatgac aggaaaaact tccattttag tggacatctt
     8881 tattgtttaa tagatcatca atttccgatg catggggtcg tgcgctcctt tcggtcgggc
     8941 gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg
     9001 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
     9061 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
     9121 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc ga
//
