LOCUS       sequence-429742-        8455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor vector for targeting an eGFP reporter to the human AGER locus 
            at the endogenous ATG start site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, 
            Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, 
            Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, 
            Kotton DN
  TITLE     Generation of human alveolar epithelial type I cells from 
            pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017.
  PUBMED    38642558
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017."
COMMENT     Sequence Label: p2705-AGER-eGFP
FEATURES             Location/Qualifiers
     source          1..8455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(32..50)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(71..87)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(84..106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(119..125,126..143,144..149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    164..185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(302..319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(473..1061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(553..572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1232..2023,2024..2092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1855..1874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2093..2197)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2223..2678)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2360..2381)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2572..2591
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2805..2827
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2819..2836
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2820..2836
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2843..2862
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2843..2861
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2881..2897
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(4613..4615,4616..4618,4619..5332)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4613..4615,4616..4618,4619..5329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4658..4679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4919..4938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5266..5287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          5383..5612
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5595..5614
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(5658..5675)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5664..5888
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5933..5949
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5984..6017)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6560..7159)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6643..6663)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7140..7159
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7171..7670)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7219..7238)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7612..7635
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(7704..7737)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7751..7767)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7751..7767)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
       61 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1021 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1081 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1141 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1201 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1261 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1321 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1381 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1441 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1501 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1561 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1621 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1681 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1741 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1801 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1861 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1921 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1981 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2041 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2101 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2161 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2221 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2281 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2341 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2401 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2461 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     2521 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     2581 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     2641 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     2701 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     2761 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     2821 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccctcgat
     2881 cgaggtcgac ggtatcgata agcttctgat ggaattagaa cttggcaaaa caatactgag
     2941 aatgaagtgt atgtggaaca gaggctgctg atctcgttct tcaggctatg aaactgacac
     3001 atttggaaac cacagtactt agaaccacaa agtgggaatc aagagaaaaa caatgatccc
     3061 acgagagatc tatagatcta tagatcatga gtgggaggaa tgagctggcc cttaatttgg
     3121 ttttgcttgt ttaaattatg atatccaact atgaaacatt atcataaagc aatagtaaag
     3181 agccttcagt aaagagcagg catttatcta atcccacccc acccccaccc ccgtagctcc
     3241 aatccttcca ttcaaaatgt aggtactctg ttctcaccct tcttaacaaa gtatgacagg
     3301 aaaaacttcc attttagtgg acatctttat tgtttaatag atcatcaatt tctgcaggtc
     3361 gactctagct agaggatccc caaatctctg taggtagttt gtccaattat gtcacaccac
     3421 agaagtaagg ttccttcaca aagagatcgc ctgacacgat ttcctgcaca ggcttgagcc
     3481 atatactcat acatcgcatc ttggccacgt tttccacggg tttcaaaatt aatctcaagt
     3541 tctacgctta acgctttcgc ctgttcccag ttattaatat attcaacgct agaactcccc
     3601 tcagcgaagg gaaggctgag cactacacgc gaagcaccat caccgaacct tttgataaac
     3661 tcttccgttc cgacttgctc catcaacggt tcagtgagac ttaaacctaa ctctttctta
     3721 atagtttcgg cattatccac ttttagtgcg agaaccttcg tcagtcctgg atacgtcact
     3781 ttgaccacgc ctccagcttt tccagagagc gggttttcat tatctacaga gtatcccgca
     3841 gcgtcgtatt tattgtcggt actataaaac cctttccaat catcgtcata atttccttgt
     3901 gtaccagatt ttggcttttg taaattccag cctgcaattg atccgggcag gactggggac
     3961 aggttggaag tgtgatgggt gggggggtgg gaggcatggg ccgggggcag ttctctcctc
     4021 acttgtaaac ttgtgtagtt tcacagaaaa aaaacaaaat gcagttttaa ataaagaaat
     4081 ttcttttttc cctgggttta gttgagaatt tttttcaaaa aacatgagaa accccagaaa
     4141 aaaaatgatt ttctttcacg aagttccaaa caggtttctc tcctgttccc cagccttgcc
     4201 ttcatgatgc aggcccaatt gcacccttgc agacaacagt ctggcctgaa ccctattgat
     4261 gcaactttgc gcaatcaaga tggggctcca gtgggtcacc aggcagccct gatggactga
     4321 tggaataaat aggatcgggg gctctgaggg aatgagaccc tagagggtac actccccatc
     4381 ccccagggaa gtgactgtac ccagaggctg gtagtaccca ggggtggggt gataattatt
     4441 tctctagtac ctgaaggact cttgtcccaa aggcatgaat tcctagcatt ccctgtgaca
     4501 agacgactga aagatggggg ctggagagag ggtgcaggca tcacctaggg cggaggccac
     4561 agcagggaga ggggcagaca gagccaggac cctggaagga agcaggacgc gtatggtgag
     4621 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4681 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     4741 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4801 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     4861 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4921 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4981 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5041 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5101 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5161 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5221 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5281 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aacatatgtc
     5341 tagatggaat taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
     5401 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
     5461 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
     5521 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
     5581 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt
     5641 cggagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5701 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5761 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5821 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5881 ctctatggct tctgaggcgg aaagaaccag ctggggctcg atcctctaga ctcgaggtcg
     5941 acggtatcga taagctagct tgggctgcag gtcgagggac ctaataactt cgtatagcat
     6001 acattatacg aagttatatt aagggttccg gatcagcttg atatcgaatt agcttctgat
     6061 ggaattagaa cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg
     6121 atctcgttct tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa
     6181 agtgggaatc aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga
     6241 gtgggaggaa tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact
     6301 atgaaacatt atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta
     6361 atcccacccc acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg
     6421 ttctcaccct tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat
     6481 tgtttaatag atcatcaatt tccgatgcat ggggtcgtgc gctcctttcg gtcgggcgct
     6541 gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc
     6601 cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt
     6661 tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc
     6721 cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg
     6781 tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt
     6841 gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca
     6901 ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg
     6961 cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg
     7021 tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg tagtcggcga
     7081 acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg
     7141 tgggcttgta ctcggtcatg gtaagctggt cgaaaggccc ggagatgagg aagaggagaa
     7201 cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg
     7261 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
     7321 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
     7381 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc
     7441 catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg
     7501 aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt
     7561 ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc
     7621 tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagaatta
     7681 attcctgcag gtcgagggac ctaataactt cgtatagcat acattatacg aagttatatt
     7741 aagggttccg gatccactag ttctagactc gagtatggcg cgccgctggt cctcagtctg
     7801 tggggtgagc cactccctca accccactga ccctccctgc agaaagcact ttaaccccac
     7861 accccagtcg tcctagaact tttcccagaa cccgaggaag tgcctttcaa ggtccctcac
     7921 ccaccctgtc caaattttgt tagccctcat tcccttccta cccctctacc atggtgctat
     7981 ctcccagggg cagtagtagg tgctcaaaac atcacagccc ggattggcga gccactggtg
     8041 ctgaagtgta agggggcccc caagaaacca ccccagcggc tggaatggaa actggtaagc
     8101 ggggctcctg ttgcagcctc ccaacttcca gggagaccag caatgatttg gatccccgtc
     8161 actctgcctc acagtccttt cccaaaggcc ttgcactgtt taggccctgc ttctctgctt
     8221 ctagaacaca ggccggacag aagcttggaa ggtcctgtct ccccagggag gaggcccctg
     8281 ggacagtgtg gctcgtgtcc ttcccaacag ctccctcttc cttccggctg tcgggatcca
     8341 ggatgagggg attttccggt gccaggcaat gaacaggaat ggaaaggaga ccaagtccaa
     8401 ctaccgagtc cgtgtctacc gtaagaattc cagggtcttc tccaaggcct gcggc
//
