LOCUS       sequence-42976-e        4622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA3k-RFP
FEATURES             Location/Qualifiers
     source          1..4622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1922..2599
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      2632..2703
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2719..2746
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2774..2791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2908..3453)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2988..3007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3567..3661)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3692..4486)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3803..3822)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4413..4432
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcttaa ttacccgact ggtctttggc accagctttt aaacgacgcc acagagtggt
       61 gcggctaatc cccagataac gcgccgcggc ggtcttatcg cccttaaagc gcgccagtac
      121 atcctgtaac gcgttcgcat ccacggttga aggcgtcagt tctgctgtgt tcaccataag
      181 ctcaggtaat aactgccgca taaattgcct gtccagcgtt ggcgcgggat cgacgcttaa
      241 aaaaagcgcc aggcgctcca tcatattacg cagttcgcga atattaccgg gccagcgcca
      301 ggccagcaaa agcggctgac actgtgtcaa tccatgacgt atcgattcgg taaacggaat
      361 ttccatcgcc gccagcgact gttttaaaaa gctttccgcc agcggcaaaa tatcagcctg
      421 ccgctcgcgc aaaggaggaa gcgtcagacg cagaatactc aggcgataaa agagatcggg
      481 gcgaaaacgt ccttgcatta tctcccgatc cagatcgcaa tgcgtagcgc tgatcacccg
      541 gacatccacc gggatcggct ggtgtccgcc gacgcgagtg acggcttttt cctccagtac
      601 gcgcaaaagt cgggtttgta acggcaaggg catttcgccg atttcatcca gaaacagcgt
      661 gccgccgtgt gcgatttcaa acagccccgc ccggcctcct cgtcgtgaac cggtaaacgc
      721 gccctcttca taaccaaaca gttccgcttc cagcaacgac tcggtaatcg cgccgcaatt
      781 gacggcgaca aagggagggg atggcttatt ctgacggtga ggctggcggt gaaagaacgt
      841 ctggtgaatc gcctgcgccg ccagctcttt tccggtccct gtttccccct gaatcagcac
      901 tgccgcacgg gagcgggcat agagcgtaat cgtctggcgg agctgctcca tttgcggcga
      961 ctggccgcgt atatcgccca gttcataccg ggtttgtaat cccttgccgg atgggtaatc
     1021 cacgcgctgg cgccgtgtca gacgggtcat atccagcgca tcatgaaaag cctgacgaac
     1081 ggtcgccgcg gaataaataa agatggcggt cattcctgcc tcttccgcca gatcggtaat
     1141 taatcccgcg ccgacgacgg cctcaatacc gttggcctta agttcgttaa tttgcccgcg
     1201 cgcgtcctct tcggtgacat agcttcgctg ttcaagacgg aggtgaaacg ttttctgaaa
     1261 ggcaagtaaa gccggaatgg tctcctgata ggtcacgata ccgatagacg aggtgagctt
     1321 tcccgctttc gccagcgcct gtaatacatc gaatccgctg ggcttgatga ggatcaccgg
     1381 tattgacagg cggcttttta aataggcccc attcgaaccc gccgcgataa tcgcgtcgca
     1441 gcgctcggtc gccagttttt tgcgaatgta ggtcaccgcc ttttcaaagc cgagttgaat
     1501 aggcgtgatg gtcgccagat gatcaaattc caggctgata tcccgaaaca gttcgaacag
     1561 acgcgttacg gagaccgtcc agatcaccgg tttatcgcta ttatcgcgcg gagcgctgtg
     1621 ggcagtcgtc atcgcagtag attcatcttt aaggggcgat tttttgtttt aaacgtgttt
     1681 cataaatgtt gcaatgaaac agggtgattc gtttcatgaa acgttagctg acacgttttt
     1741 ttttccctta atcgcgctta ttcataacag aaatgactgt aattacctgt ttttaaatct
     1801 cattgtattt aattttctcg ctgcctcttt gcctggcata gcctttgctt tggtgaatac
     1861 atcttgaata acaatttact aacatggaat tcaaaagatc ttttaagaag gagatataca
     1921 tatggcgagt agcgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     1981 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     2041 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     2101 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     2161 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     2221 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     2281 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     2341 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     2401 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     2461 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     2521 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     2581 tcactccacc ggtgcttaag gatccaaact cgagtaagga tctccaggca tcaaataaaa
     2641 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2701 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctaggga
     2761 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     2821 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     2881 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     2941 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     3001 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     3061 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     3121 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     3181 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     3241 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     3301 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     3361 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     3421 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
     3481 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     3541 cgatataagt tgttactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     3601 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     3661 ccaagcgagc tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     3721 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     3781 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     3841 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     3901 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
     3961 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     4021 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     4081 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     4141 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     4201 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     4261 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     4321 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     4381 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     4441 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     4501 atcctgtctc ttgatcagat catgatcccc tgcgccatca gatccttggc ggcaagaaag
     4561 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
     4621 cc
//
