LOCUS       sequence-42979-e        6044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS3k-RFP
FEATURES             Location/Qualifiers
     source          1..6044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1922..2599
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      2632..2703
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2719..2746
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3277..4227)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(4989..5083)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5114..5908)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5225..5244)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5835..5854
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcttaa ttacccgact ggtctttggc accagctttt aaacgacgcc acagagtggt
       61 gcggctaatc cccagataac gcgccgcggc ggtcttatcg cccttaaagc gcgccagtac
      121 atcctgtaac gcgttcgcat ccacggttga aggcgtcagt tctgctgtgt tcaccataag
      181 ctcaggtaat aactgccgca taaattgcct gtccagcgtt ggcgcgggat cgacgcttaa
      241 aaaaagcgcc aggcgctcca tcatattacg cagttcgcga atattaccgg gccagcgcca
      301 ggccagcaaa agcggctgac actgtgtcaa tccatgacgt atcgattcgg taaacggaat
      361 ttccatcgcc gccagcgact gttttaaaaa gctttccgcc agcggcaaaa tatcagcctg
      421 ccgctcgcgc aaaggaggaa gcgtcagacg cagaatactc aggcgataaa agagatcggg
      481 gcgaaaacgt ccttgcatta tctcccgatc cagatcgcaa tgcgtagcgc tgatcacccg
      541 gacatccacc gggatcggct ggtgtccgcc gacgcgagtg acggcttttt cctccagtac
      601 gcgcaaaagt cgggtttgta acggcaaggg catttcgccg atttcatcca gaaacagcgt
      661 gccgccgtgt gcgatttcaa acagccccgc ccggcctcct cgtcgtgaac cggtaaacgc
      721 gccctcttca taaccaaaca gttccgcttc cagcaacgac tcggtaatcg cgccgcaatt
      781 gacggcgaca aagggagggg atggcttatt ctgacggtga ggctggcggt gaaagaacgt
      841 ctggtgaatc gcctgcgccg ccagctcttt tccggtccct gtttccccct gaatcagcac
      901 tgccgcacgg gagcgggcat agagcgtaat cgtctggcgg agctgctcca tttgcggcga
      961 ctggccgcgt atatcgccca gttcataccg ggtttgtaat cccttgccgg atgggtaatc
     1021 cacgcgctgg cgccgtgtca gacgggtcat atccagcgca tcatgaaaag cctgacgaac
     1081 ggtcgccgcg gaataaataa agatggcggt cattcctgcc tcttccgcca gatcggtaat
     1141 taatcccgcg ccgacgacgg cctcaatacc gttggcctta agttcgttaa tttgcccgcg
     1201 cgcgtcctct tcggtgacat agcttcgctg ttcaagacgg aggtgaaacg ttttctgaaa
     1261 ggcaagtaaa gccggaatgg tctcctgata ggtcacgata ccgatagacg aggtgagctt
     1321 tcccgctttc gccagcgcct gtaatacatc gaatccgctg ggcttgatga ggatcaccgg
     1381 tattgacagg cggcttttta aataggcccc attcgaaccc gccgcgataa tcgcgtcgca
     1441 gcgctcggtc gccagttttt tgcgaatgta ggtcaccgcc ttttcaaagc cgagttgaat
     1501 aggcgtgatg gtcgccagat gatcaaattc caggctgata tcccgaaaca gttcgaacag
     1561 acgcgttacg gagaccgtcc agatcaccgg tttatcgcta ttatcgcgcg gagcgctgtg
     1621 ggcagtcgtc atcgcagtag attcatcttt aaggggcgat tttttgtttt aaacgtgttt
     1681 cataaatgtt gcaatgaaac agggtgattc gtttcatgaa acgttagctg acacgttttt
     1741 ttttccctta atcgcgctta ttcataacag aaatgactgt aattacctgt ttttaaatct
     1801 cattgtattt aattttctcg ctgcctcttt gcctggcata gcctttgctt tggtgaatac
     1861 atcttgaata acaatttact aacatggaat tcaaaagatc ttttaagaag gagatataca
     1921 tatggcgagt agcgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     1981 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     2041 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     2101 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     2161 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     2221 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     2281 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     2341 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     2401 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     2461 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     2521 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     2581 tcactccacc ggtgcttaag gatccaaact cgagtaagga tctccaggca tcaaataaaa
     2641 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2701 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctagggt
     2761 acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc
     2821 agatccggct tcagccggtt tgccggctga aagcgctatt tcttccagaa ttgccatgat
     2881 tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga ttcgataagc
     2941 agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg
     3001 tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct attaggtgtt
     3061 acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt gaatgcacca
     3121 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
     3181 gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt
     3241 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
     3301 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
     3361 atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
     3421 agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat
     3481 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
     3541 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
     3601 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
     3661 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
     3721 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
     3781 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
     3841 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
     3901 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
     3961 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
     4021 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     4081 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     4141 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     4201 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     4261 ataccaattg agatgggcta gtcaatgata attactagtc cttttcccgg gtgatctggg
     4321 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     4381 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     4441 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     4501 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     4561 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     4621 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     4681 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     4741 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     4801 ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt cagcagttcc
     4861 tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc attcagactg
     4921 gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt actgtcccta
     4981 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     5041 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctctcgaac
     5101 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     5161 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     5221 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     5281 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     5341 cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca
     5401 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     5461 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     5521 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     5581 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     5641 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     5701 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
     5761 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     5821 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     5881 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
     5941 atcatgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     6001 agggcttccc aaccttacca gagggcgccc cagctggcaa ttcc
//
