LOCUS       sequence-429912-        8546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green and hygromycin resistant lentiviral vector plasmid for 
            multiplexed receptor pseudovirus experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Shukla N, Roelle SM, Snell JC, DelSignore O, Bruchez AM, Matreyek KA
  TITLE     Pseudotyped virus infection of multiplexed ACE2 libraries reveals 
            SARS-CoV-2 variant shifts in receptor usage.
  JOURNAL   PLoS Pathog. 2024 May 20;20(5):e1012044. doi: 
            10.1371/journal.ppat.1012044.
  PUBMED    38768238
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2024-05-20"; volume: "20(5):e1012044. doi"; pages: 
            " 10.1371/journal.ppat.1012044"
COMMENT     Sequence Label: G1088E_pLenti-CMV-mNeonGreen-2A-HygroR
FEATURES             Location/Qualifiers
     source          1..8546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(128..139)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             382..615
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    687..808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(724..743)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     778..797
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      848..983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1003..1022)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1004..1022)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1032..1049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1032..1048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1041..1063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1190..1645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1277..1296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1487..1508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1671..1775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1776..1844,1845..2636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1994..2013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2807..3395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3296..3315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3549..3566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3683..3704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3719..3724,3725..3742,3743..3749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3757..3773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3762..3784
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3781..3797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3781..3797
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3816..3836
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3818..3836
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3864..4090
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4091..4271
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4318..4443
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4936..5169
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5354..5398
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5665..5781
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        5804..6107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6108..6311
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6262..6282
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6308..6332
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6343..6352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6604..6613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7063..7119
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7120..8145
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7120..8145
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8148..8172)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(8203..8546,1..245)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8256..8276)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
       61 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
      121 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
      181 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
      241 cccgcctgga attctgcaga tatccggtta gtaatgagtt tggaattaat tctgtgcaca
      301 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
      361 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
      421 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      481 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      541 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      601 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
      661 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
      721 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      781 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
      841 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      901 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
      961 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     1021 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1081 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1141 cgcaccgatc acccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     1201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     1381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     1441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     1501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     1561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     1621 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1681 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1741 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1801 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1861 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1921 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1981 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2041 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2101 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2161 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2221 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2281 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2341 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2401 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2461 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2521 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2581 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2641 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2701 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2941 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3481 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3541 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3601 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3661 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     3721 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     3781 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     3841 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     3901 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     3961 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     4021 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     4081 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4141 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4201 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4261 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     4321 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     4381 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     4441 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     4501 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     4561 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     4621 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     4681 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     4741 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     4801 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     4861 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     4921 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     4981 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     5041 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     5101 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     5161 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     5221 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     5281 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     5341 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     5401 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     5461 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     5521 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     5581 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     5641 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     5701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     5761 aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg
     5821 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5881 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5941 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     6001 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     6061 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6121 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6181 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6241 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6301 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccaccat ggtgtccaag
     6361 ggcgaggagg acaacatggc ctccctgccc gctacccacg agctgcacat tttcggctcc
     6421 attaacggcg tggacttcga catggtgggc cagggcacag gcaacccaaa cgacggctac
     6481 gaggagctga acctgaagtc tacaaagggc gacctgcagt tctctccatg gatcctggtg
     6541 ccccacatcg gctacggctt ccaccagtac ctcccctacc ccgacggcat gtcccctttc
     6601 caggccgcca tggtggacgg cagcggctac caggtgcaca ggaccatgca gttcgaggac
     6661 ggcgctagcc tgacagtgaa ctaccgctac acctacgagg gcagccacat taagggcgag
     6721 gctcaggtga agggcacagg cttccctgcc gacggccctg tgatgacaaa ctctctgaca
     6781 gccgctgact ggtgcagatc taagaagacc tacccaaacg acaagaccat cattagcacc
     6841 ttcaagtggt cttacaccac cggcaacggc aagcggtaca gaagcacagc caggacaaca
     6901 tacacattcg ctaagcccat ggctgctaac tacctgaaga accagcccat gtacgtgttc
     6961 aggaagacag agctgaagca cagcaagaca gagctcaact tcaaggagtg gcagaaggct
     7021 ttcaccgacg tgatgggcat ggacgagctg tacaagagcg gagctactaa cttcagcctg
     7081 ctgaagcagg ctggagacgt ggaggagaac cctggaccta tgaaaaagcc tgaactcacc
     7141 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     7201 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     7261 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     7321 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     7381 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     7441 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     7501 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     7561 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     7621 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     7681 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     7741 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     7801 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     7861 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     7921 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     7981 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     8041 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     8101 agtggaaacc gacgccccag cactcgtccg agggcaaagg aataatcacc cagctttctt
     8161 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac
     8221 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8281 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8341 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8401 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8461 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8521 atcgccgcct gccttgcccg ctgctg
//
