LOCUS       sequence-429914-        9068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nonsense mediated decay GFP P2A RFP reporter with two b-globin 
            introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Inglis AJ, Guna A, Galvez-Merchan A, Pal A, Esantsi TK, Keys HR, 
            Frenkel EM, Oania R, Weissman JS, Voorhees RM
  TITLE     Coupled protein quality control during nonsense-mediated mRNA decay.
  JOURNAL   J Cell Sci. 2023 May 15;136(10):jcs261216. doi: 10.1242/jcs.261216. 
            Epub 2023 May 23.
  PUBMED    37218462
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-05-15"; volume: "136(10):jcs261216. doi"; pages: 
            " 10.1242/jcs.261216. Epub 2023 May 23"
COMMENT     Sequence Label: NMD2
FEATURES             Location/Qualifiers
     source          1..9068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1706..1762
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3659..3676
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3660..3676
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5020..5037)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5026..5250
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5383..5402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5534..5581
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5589..6611
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5589..6611
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6741..6862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6778..6797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6832..6851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6911..6927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6911..6927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6924..6946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6935..6951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6959..6965,6966..6983,6984..6989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7004..7025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7142..7159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7313..7901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7393..7412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8072..8863,8864..8932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8695..8714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8933..9037)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttacggtac catggtgtct aaaggtgaag aactttttac
     1021 gggtgtcgtc cccatcctgg ttgaattgga cggagacgtg aatggccata aattttcagt
     1081 gagcggtgaa ggagagggag acgccactta cggtaaactt accttgaaat ttatctgcac
     1141 taccgggaaa ctgcccgtac cctggccgac gctcgtaaca acgcttacct acggcgtgca
     1201 atgttttagc agatacccag accatatgaa gcagcacgat tttttcaaga gtgcaatgcc
     1261 tgaggggtac gtacaggaaa ggacgatctt tttcaaagac gatggtaatt ataaaacgcg
     1321 ggctgaggtt aaatttgaag gagacacgct cgtgaatcga atagagctga agggaatcga
     1381 cttcaaggaa gacggtaaca tcctgggcca taaactcgaa tataattaca attcacacaa
     1441 cgtctatatc atggcagata aacagaagaa tggcatcaaa gtcaatttta agatccggca
     1501 taatatcgag gacggatcag tacagcttgc cgaccattac caacagaata caccgatagg
     1561 agacggtccc gtcctcttgc cagataatca ctatttgagc acacaatccg ctctttctaa
     1621 agatccgaat gaaaagcgcg atcatatggt cttgaaagag ttcgtcactg ccgccggcat
     1681 aaccggcgga tccgccggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
     1741 cgtggaggag aaccctggac ctggcaccgg ttccaacatg gcgattatca aggaattcat
     1801 gaggttcaaa gttcacatgg agggatctgt aaacggccac gagttcgaaa tcgagggaga
     1861 gggggagggt cgaccatatg aaggaacaca gaccgctaaa ttgaaggtta ctaaaggtgg
     1921 accacttcct ttcgcatggg acattcttag cccccagttt atgtatggtt ccaaggcgta
     1981 tgtcaaacat cccgcagaca ttcctgatta ccttaaactg agttttccag aggggttcaa
     2041 atgggagcgc gtcatgaact ttgaagatgg gggcgttgtt acagtgactc aggactcttc
     2101 tttgcaagat ggagagttta tatataaggt gaaacttcgc ggaaccaact tcccaagcga
     2161 tggcccagtc atgcagaaga aaactatggg ttgggaagcc agtagcgagc ggatgtaccc
     2221 agaggacggc gcacttaagg gtgagattaa gcagcggctg aaactcaaag acggaggcca
     2281 ttatgacgcg gaagttaaaa cgacgtacaa agctaaaaag cctgtccaat tgccaggcgc
     2341 gtacaatgtc aatattaaat tggacattac cagtcacaac gaagattaca caatagtgga
     2401 gcaatacgaa cgcgcagagg gacgccattc aacagggtaa gcggccgcgg gctgctggtg
     2461 gtctaccctt ggacccagag gttctttgag tcctttgggg atctgtccac tcctgatgct
     2521 gttatgggca accctaaggt gaaggctcat ggcaagaaag tgctcggtgc ctttagtgat
     2581 ggcctggctc acctggacaa cctcaagggc acctttgcca cactgagtga gctgcactgt
     2641 gacaagctgc acgtggatcc tgagaacttc agggtgagtc tatgggaccc ttgatgtttt
     2701 ctttcccctt cttttctatg gttaagttca tgtcatagga aggggagaag taacagggta
     2761 cagtttagaa tgggaaacag acgaatgatt gcatcagtgt ggaagtctca ggatcgtttt
     2821 agtttctttt atttgctgtt cataacaatt gttttctttt gtttaattct tgctttcttt
     2881 ttttttcttc tccgcaattt ttactattat acttaatgcc ttaacattgt gtataacaaa
     2941 aggaaatatc tctgagatac attaagtaac ttaaaaaaaa actttacaca gtctgcctag
     3001 tacattacta tttggaatat atgtgtgctt atttgcatat tcataatctc cctactttat
     3061 tttcttttat ttttaattga tacataatca ttatacatat ttatgggtta aagtgtaatg
     3121 ttttaatatg tgtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa
     3181 aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc
     3241 tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa
     3301 taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc
     3361 atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta
     3421 ccattctgct tttattttat ggttgggata aggctggatt attctgagtc caagctaggc
     3481 ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct
     3541 ggtctgtgtg ctggcccatc actttggcaa agaattcacc ccaccagtgc aggctgccta
     3601 tcagaaagtg gtggctggtg tggctaatgc cctggcccac aagtatcact aactcgagtg
     3661 taaaacgacg gccagtgctg ctggtggtct acccttggac ccagaggttc tttgagtcct
     3721 ttggggatct gtccactcct gatgctgtta tgggcaaccc taaggtgaag gctcatggca
     3781 agaaagtgct cggtgccttt agtgatggcc tggctcacct ggacaacctc aagggcacct
     3841 ttgccacact gagtgagctg cactgtgaca agctgcacgt ggatcctgag aacttcaggg
     3901 tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc
     3961 ataggaaggg gagaagtaac agggtacagt ttagaatggg aaacagacga atgattgcat
     4021 cagtgtggaa gtctcaggat cgttttagtt tcttttattt gctgttcata acaattgttt
     4081 tcttttgttt aattcttgct ttcttttttt ttcttctccg caatttttac tattatactt
     4141 aatgccttaa cattgtgtat aacaaaagga aatatctctg agatacatta agtaacttaa
     4201 aaaaaaactt tacacagtct gcctagtaca ttactatttg gaatatatgt gtgcttattt
     4261 gcatattcat aatctcccta ctttattttc ttttattttt aattgataca taatcattat
     4321 acatatttat gggttaaagt gtaatgtttt aatatgtgta cacatattga ccaaatcagg
     4381 gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact tttttgttta
     4441 tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat acaatgtatc
     4501 atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa ggcaatagca
     4561 atatttctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga ggtttcatat
     4621 tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt gggataaggc
     4681 tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc tcttatcttc
     4741 ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg cccatcactt tggcaaagaa
     4801 ttcaccccac cagtgcaggc tgcctatcag aaagtggtgg ctggtgtggc taatgccctg
     4861 gcccacaagt atcactaagc tcgctttctt gctgtccaat ttctattaaa ggttcctttg
     4921 ttccctaagt ccaactacta aactggggga tattatgaag ggccttgagc atctggattc
     4981 tgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     5041 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5101 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5161 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5221 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     5281 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5341 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5401 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5461 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5521 gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     5581 ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     5641 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     5701 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     5761 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     5821 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     5881 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     5941 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     6001 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     6061 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     6121 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     6181 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     6241 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     6301 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     6361 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     6421 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     6481 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     6541 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     6601 caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6661 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6721 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6841 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8641 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8701 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8761 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8821 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8881 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8941 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9001 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9061 ctgacgtc
//
