LOCUS       sequence-429915-       12390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nonsense mediated decay GFP P2A RFP reporter with two b-globin 
            introns in lenti backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12390)
  AUTHORS   Inglis AJ, Guna A, Galvez-Merchan A, Pal A, Esantsi TK, Keys HR, 
            Frenkel EM, Oania R, Weissman JS, Voorhees RM
  TITLE     Coupled protein quality control during nonsense-mediated mRNA decay.
  JOURNAL   J Cell Sci. 2023 May 15;136(10):jcs261216. doi: 10.1242/jcs.261216. 
            Epub 2023 May 23.
  PUBMED    37218462
REFERENCE   2  (bases 1 to 12390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-05-15"; volume: "136(10):jcs261216. doi"; pages: 
            " 10.1242/jcs.261216. Epub 2023 May 23"
COMMENT     Sequence Label: NMD2_lenti
FEATURES             Location/Qualifiers
     source          1..12390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    312..545
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             730..774
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1059..1176
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    1213..1437
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1426..1443
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(2787..2804)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2787..2803)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4701..4757)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(5503..5852)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(5540..5564)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5610..5628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5646..5664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5682..5700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5718..5736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5754..5772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5790..5808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5826..5844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     LTR             5961..6194
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6219..6237)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6220..6237)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6527..6546)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6760..6779)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6879..6901
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6939..6957)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7025..7129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7130..7198,7199..7990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7348..7367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8161..8749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8650..8669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8903..8920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8990..9010)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8995..9324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9175..9310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10458..10523
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10653..10673
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11098..11232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11135..11154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11189..11208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11400..12033
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12080..12205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
       61 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
      121 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
      181 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
      241 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
      301 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
      361 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
      421 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
      481 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
      541 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
      601 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
      661 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
      721 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
      781 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
      841 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
      901 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
      961 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     1021 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     1081 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     1141 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     1201 gatccagttt atccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     1261 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     1321 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttctaggg
     1381 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     1441 aggctgatca gcgggtttaa acgggccctc tagactcgag cagaatccag atgctcaagg
     1501 cccttcataa tatcccccag tttagtagtt ggacttaggg aacaaaggaa cctttaatag
     1561 aaattggaca gcaagaaagc gagcttagtg atacttgtgg gccagggcat tagccacacc
     1621 agccaccact ttctgatagg cagcctgcac tggtggggtg aattctttgc caaagtgatg
     1681 ggccagcaca cagaccagca cgttgcccag gagctgtggg aggaagataa gaggtatgaa
     1741 catgattagc aaaagggcct agcttggact cagaataatc cagccttatc ccaaccataa
     1801 aataaaagca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttacat
     1861 cagttacaat ttatatgcag aaatatttat atgcagaaat attgctattg ccttaaccca
     1921 gaaattatca ctgttattct ttagaatggt gcaaagaggc atgatacatt gtatcattat
     1981 tgccctgaaa gaaagagatt agggaaagta ttagaaataa gataaacaaa aaagtatatt
     2041 aaaagaagaa agcatttttt aaaattacaa atgcaaaatt accctgattt ggtcaatatg
     2101 tgtacacata ttaaaacatt acactttaac ccataaatat gtataatgat tatgtatcaa
     2161 ttaaaaataa aagaaaataa agtagggaga ttatgaatat gcaaataagc acacatatat
     2221 tccaaatagt aatgtactag gcagactgtg taaagttttt ttttaagtta cttaatgtat
     2281 ctcagagata tttccttttg ttatacacaa tgttaaggca ttaagtataa tagtaaaaat
     2341 tgcggagaag aaaaaaaaag aaagcaagaa ttaaacaaaa gaaaacaatt gttatgaaca
     2401 gcaaataaaa gaaactaaaa cgatcctgag acttccacac tgatgcaatc attcgtctgt
     2461 ttcccattct aaactgtacc ctgttacttc tccccttcct atgacatgaa cttaaccata
     2521 gaaaagaagg ggaaagaaaa catcaagggt cccatagact caccctgaag ttctcaggat
     2581 ccacgtgcag cttgtcacag tgcagctcac tcagtgtggc aaaggtgccc ttgaggttgt
     2641 ccaggtgagc caggccatca ctaaaggcac cgagcacttt cttgccatga gccttcacct
     2701 tagggttgcc cataacagca tcaggagtgg acagatcccc aaaggactca aagaacctct
     2761 gggtccaagg gtagaccacc agcagcactg gccgtcgttt tacactcgag ttagtgatac
     2821 ttgtgggcca gggcattagc cacaccagcc accactttct gataggcagc ctgcactggt
     2881 ggggtgaatt ctttgccaaa gtgatgggcc agcacacaga ccagcacgtt gcccaggagc
     2941 tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct tggactcaga
     3001 ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga ttgtagctgc
     3061 tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat atttatatgc
     3121 agaaatattg ctattgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     3181 agaggcatga tacattgtat cattattgcc ctgaaagaaa gagattaggg aaagtattag
     3241 aaataagata aacaaaaaag tatattaaaa gaagaaagca ttttttaaaa ttacaaatgc
     3301 aaaattaccc tgatttggtc aatatgtgta cacatattaa aacattacac tttaacccat
     3361 aaatatgtat aatgattatg tatcaattaa aaataaaaga aaataaagta gggagattat
     3421 gaatatgcaa ataagcacac atatattcca aatagtaatg tactaggcag actgtgtaaa
     3481 gttttttttt aagttactta atgtatctca gagatatttc cttttgttat acacaatgtt
     3541 aaggcattaa gtataatagt aaaaattgcg gagaagaaaa aaaaagaaag caagaattaa
     3601 acaaaagaaa acaattgtta tgaacagcaa ataaaagaaa ctaaaacgat cctgagactt
     3661 ccacactgat gcaatcattc gtctgtttcc cattctaaac tgtaccctgt tacttctccc
     3721 cttcctatga catgaactta accatagaaa agaaggggaa agaaaacatc aagggtccca
     3781 tagactcacc ctgaagttct caggatccac gtgcagcttg tcacagtgca gctcactcag
     3841 tgtggcaaag gtgcccttga ggttgtccag gtgagccagg ccatcactaa aggcaccgag
     3901 cactttcttg ccatgagcct tcaccttagg gttgcccata acagcatcag gagtggacag
     3961 atccccaaag gactcaaaga acctctgggt ccaagggtag accaccagca gccagcggcc
     4021 gcttaccctg ttgaatggcg tccctctgcg cgttcgtatt gctccactat tgtgtaatct
     4081 tcgttgtgac tggtaatgtc caatttaata ttgacattgt acgcgcctgg caattggaca
     4141 ggctttttag ctttgtacgt cgttttaact tccgcgtcat aatggcctcc gtctttgagt
     4201 ttcagccgct gcttaatctc acccttaagt gcgccgtcct ctgggtacat ccgctcgcta
     4261 ctggcttccc aacccatagt tttcttctgc atgactgggc catcgcttgg gaagttggtt
     4321 ccgcgaagtt tcaccttata tataaactct ccatcttgca aagaagagtc ctgagtcact
     4381 gtaacaacgc ccccatcttc aaagttcatg acgcgctccc atttgaaccc ctctggaaaa
     4441 ctcagtttaa ggtaatcagg aatgtctgcg ggatgtttga catacgcctt ggaaccatac
     4501 ataaactggg ggctaagaat gtcccatgcg aaaggaagtg gtccaccttt agtaaccttc
     4561 aatttagcgg tctgtgttcc ttcatatggt cgaccctccc cctctccctc gatttcgaac
     4621 tcgtggccgt ttacagatcc ctccatgtga actttgaacc tcatgaattc cttgataatc
     4681 gccatgttgg aaccggtgcc aggtccaggg ttctcctcca cgtctccagc ctgcttcagc
     4741 aggctgaagt tagtagctcc gcttccggcg gatccgccgg ttatgccggc ggcagtgacg
     4801 aactctttca agaccatatg atcgcgcttt tcattcggat ctttagaaag agcggattgt
     4861 gtgctcaaat agtgattatc tggcaagagg acgggaccgt ctcctatcgg tgtattctgt
     4921 tggtaatggt cggcaagctg tactgatccg tcctcgatat tatgccggat cttaaaattg
     4981 actttgatgc cattcttctg tttatctgcc atgatataga cgttgtgtga attgtaatta
     5041 tattcgagtt tatggcccag gatgttaccg tcttccttga agtcgattcc cttcagctct
     5101 attcgattca cgagcgtgtc tccttcaaat ttaacctcag cccgcgtttt ataattacca
     5161 tcgtctttga aaaagatcgt cctttcctgt acgtacccct caggcattgc actcttgaaa
     5221 aaatcgtgct gcttcatatg gtctgggtat ctgctaaaac attgcacgcc gtaggtaagc
     5281 gttgttacga gcgtcggcca gggtacgggc agtttcccgg tagtgcagat aaatttcaag
     5341 gtaagtttac cgtaagtggc gtctccctct ccttcaccgc tcactgaaaa tttatggcca
     5401 ttcacgtctc cgtccaattc aaccaggatg gggacgacac ccgtaaaaag ttcttcacct
     5461 ttagacacca tggtaccatg ctagtttgca acgtatggta ccaagtataa gacaaaagtg
     5521 ttgtggaatt gctccaggcg atctgacggt tcactaaacg agctctgctt ttataggcgc
     5581 ccaccgtaca cgcctacctc gacatacgtt ctctatcact gatagggagt aaactggata
     5641 tacgttctct atcactgata gggagtaaac tgtaggtacg ttctctatca ctgataggga
     5701 gtaaactggt catacgttct ctatcactga tagggagtaa actccttata cgttctctat
     5761 cactgatagg gagtaaagtc tgcatacgtt ctctatcact gatagggagt aaactcttca
     5821 tacgttctct atcactgata gggagtaaac tcgagtgaag acgaaagggg tttaaacccg
     5881 ctgatcagcc tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5941 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6001 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6061 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6121 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6181 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     6241 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     6301 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     6361 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     6421 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     6481 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     6541 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     6601 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     6661 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     6721 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     6781 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     6841 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     6901 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     6961 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7141 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7201 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7261 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7321 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7381 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7441 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7501 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7561 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7621 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7681 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7741 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7801 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7861 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7921 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7981 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8041 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8821 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8881 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8941 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     9001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9241 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9301 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     9361 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     9421 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     9481 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     9541 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     9601 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     9661 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     9721 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     9781 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     9841 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     9901 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     9961 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
    10021 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
    10081 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
    10141 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
    10201 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
    10261 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
    10321 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
    10381 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
    10441 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
    10501 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
    10561 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
    10621 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
    10681 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
    10741 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
    10801 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
    10861 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
    10921 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
    10981 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    11041 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    11101 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    11161 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11221 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    11281 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    11341 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
    11401 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
    11461 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
    11521 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
    11581 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
    11641 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
    11701 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
    11761 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
    11821 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
    11881 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    11941 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    12001 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
    12061 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
    12121 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
    12181 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
    12241 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
    12301 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
    12361 tagacaaata ctgggacagc tacaaccatc
//
