LOCUS       sequence-429916-        9858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP P2A RFP control reporter in a lenti backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Inglis AJ, Guna A, Galvez-Merchan A, Pal A, Esantsi TK, Keys HR, 
            Frenkel EM, Oania R, Weissman JS, Voorhees RM
  TITLE     Coupled protein quality control during nonsense-mediated mRNA decay.
  JOURNAL   J Cell Sci. 2023 May 15;136(10):jcs261216. doi: 10.1242/jcs.261216. 
            Epub 2023 May 23.
  PUBMED    37218462
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-05-15"; volume: "136(10):jcs261216. doi"; pages: 
            " 10.1242/jcs.261216. Epub 2023 May 23"
COMMENT     Sequence Label: GPR_control_lenti
FEATURES             Location/Qualifiers
     source          1..9858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(109..128)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(342..361)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     461..483
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(521..539)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        607..711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(712..780,781..1572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(930..949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1743..2331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2232..2251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2485..2502
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2572..2592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2577..2906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2757..2892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4040..4105
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4235..4255
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4680..4814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4717..4736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4771..4790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4982..5615
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5662..5787
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6284..6517
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6702..6746
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7031..7148
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    7185..7409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7398..7415
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8141..8197)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(8943..9292)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(8980..9004)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9050..9068
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9086..9104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9122..9140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9158..9176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9194..9212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9230..9248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9266..9284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     LTR             9401..9634
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9659..9677)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9660..9677)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
       61 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      121 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      181 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      241 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      301 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
      361 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      421 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
      481 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
      541 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      601 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      661 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      721 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      781 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      841 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      901 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      961 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1021 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1081 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1141 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1201 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1261 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1321 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1381 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1441 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1501 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1561 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1621 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1681 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1741 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1801 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1861 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1921 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1981 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2041 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2101 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2161 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2221 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2281 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2341 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2401 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2461 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2521 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     2581 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2641 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2701 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2761 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2821 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     2881 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
     2941 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     3001 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     3061 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
     3121 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
     3181 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
     3241 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
     3301 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
     3361 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
     3421 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
     3481 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
     3541 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
     3601 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
     3661 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
     3721 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
     3781 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
     3841 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
     3901 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
     3961 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     4021 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     4081 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     4141 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     4201 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     4261 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     4321 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     4381 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     4441 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     4501 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     4561 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4621 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4801 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     4861 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     4921 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     4981 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     5041 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     5101 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     5161 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     5221 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     5281 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     5341 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     5401 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     5461 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5521 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5581 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     5641 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     5701 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     5761 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     5821 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     5881 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     5941 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     6001 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     6061 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     6121 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     6181 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     6241 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     6301 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     6361 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     6421 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     6481 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     6541 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     6601 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     6661 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     6721 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     6781 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     6841 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     6901 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     6961 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat
     7021 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     7081 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     7141 aaaattttcg ggtttattac agggacagca gagatccagt ttatccatag agcccaccgc
     7201 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc
     7261 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta
     7321 ggaaaggaca gtgggagtgg caccttctag ggtcaaggaa ggcacggggg aggggcaaac
     7381 aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgggttt aaacgggccc
     7441 tctagactcg agtagcggcc gcttaccctg ttgaatggcg tccctctgcg cgttcgtatt
     7501 gctccactat tgtgtaatct tcgttgtgac tggtaatgtc caatttaata ttgacattgt
     7561 acgcgcctgg caattggaca ggctttttag ctttgtacgt cgttttaact tccgcgtcat
     7621 aatggcctcc gtctttgagt ttcagccgct gcttaatctc acccttaagt gcgccgtcct
     7681 ctgggtacat ccgctcgcta ctggcttccc aacccatagt tttcttctgc atgactgggc
     7741 catcgcttgg gaagttggtt ccgcgaagtt tcaccttata tataaactct ccatcttgca
     7801 aagaagagtc ctgagtcact gtaacaacgc ccccatcttc aaagttcatg acgcgctccc
     7861 atttgaaccc ctctggaaaa ctcagtttaa ggtaatcagg aatgtctgcg ggatgtttga
     7921 catacgcctt ggaaccatac ataaactggg ggctaagaat gtcccatgcg aaaggaagtg
     7981 gtccaccttt agtaaccttc aatttagcgg tctgtgttcc ttcatatggt cgaccctccc
     8041 cctctccctc gatttcgaac tcgtggccgt ttacagatcc ctccatgtga actttgaacc
     8101 tcatgaattc cttgataatc gccatgttgg aaccggtgcc aggtccaggg ttctcctcca
     8161 cgtctccagc ctgcttcagc aggctgaagt tagtagctcc gcttccggcg gatccgccgg
     8221 ttatgccggc ggcagtgacg aactctttca agaccatatg atcgcgcttt tcattcggat
     8281 ctttagaaag agcggattgt gtgctcaaat agtgattatc tggcaagagg acgggaccgt
     8341 ctcctatcgg tgtattctgt tggtaatggt cggcaagctg tactgatccg tcctcgatat
     8401 tatgccggat cttaaaattg actttgatgc cattcttctg tttatctgcc atgatataga
     8461 cgttgtgtga attgtaatta tattcgagtt tatggcccag gatgttaccg tcttccttga
     8521 agtcgattcc cttcagctct attcgattca cgagcgtgtc tccttcaaat ttaacctcag
     8581 cccgcgtttt ataattacca tcgtctttga aaaagatcgt cctttcctgt acgtacccct
     8641 caggcattgc actcttgaaa aaatcgtgct gcttcatatg gtctgggtat ctgctaaaac
     8701 attgcacgcc gtaggtaagc gttgttacga gcgtcggcca gggtacgggc agtttcccgg
     8761 tagtgcagat aaatttcaag gtaagtttac cgtaagtggc gtctccctct ccttcaccgc
     8821 tcactgaaaa tttatggcca ttcacgtctc cgtccaattc aaccaggatg gggacgacac
     8881 ccgtaaaaag ttcttcacct ttagacacca tggtaccatg ctagtttgca acgtatggta
     8941 ccaagtataa gacaaaagtg ttgtggaatt gctccaggcg atctgacggt tcactaaacg
     9001 agctctgctt ttataggcgc ccaccgtaca cgcctacctc gacatacgtt ctctatcact
     9061 gatagggagt aaactggata tacgttctct atcactgata gggagtaaac tgtagatacg
     9121 ttctctatca ctgataggga gtaaactggt catacgttct ctatcactga tagggagtaa
     9181 actccttata cgttctctat cactgatagg gagtaaagtc tgcatacgtt ctctatcact
     9241 gatagggagt aaactcttca tacgttctct atcactgata gggagtaaac tcgagtgaag
     9301 acgaaagggg tttaaacccg ctgatcagcc tcgactttaa gaccaatgac ttacaaggca
     9361 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9421 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9601 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     9661 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     9721 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     9781 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     9841 ctggtaacgc cgtcccgc
//
