LOCUS       sequence-429919-       10450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike for JD.1 pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 JD.1
FEATURES             Location/Qualifiers
     source          1..10450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(222..424)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(251..271)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        426..729
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    810..858
                     /note="synthetic polyadenylation signal"
     terminator      876..922
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    927..998
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1150..1286)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1299..2090,2091..2159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1922..1941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2905..3493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3394..3413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3689..3913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3924..4718)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4035..4054)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4645..4664
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4732..4928)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4746..4881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    4973..5044
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5089..5225)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(5255..5301)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5416..5537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5453..5472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5507..5526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5590..6178
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5696..5707
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6105..6125
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          join(10063..10450,1..88)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 gtaccctgtt acttctcccc ttcctatgac atgaacttaa ccatagaaaa gaaggggaaa
       61 gaaaacatca agggtcccat agactcaccc tgaagttctc aggatccgag ctcggtacca
      121 catgtaagct tcgaggggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag
      181 cgtggatggc gtctccaggc gatctgacgg ttcactaaac cagctctgct tatatagacc
      241 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      301 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
      361 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
      421 atcagcatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
      481 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
      541 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
      601 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
      661 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
      721 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactag
      781 atctggctac actcgatcag cagttagata ataaaatcgc tatccatcga agatggatgt
      841 gtgttggttt tttgtgtgtg taacgcaacg attgatagca taaccccttg gggcctctaa
      901 acgggtcttg aggggttttt tgaattgaga ggtagctgaa gctgctgtta tgaccactag
      961 agggaagaag atacctgtgg agctaatggt ccaagatgtc gggagccgcg atcgctgcca
     1021 aaaaggtaag ctgacgatag ctgacgagat tcgatgcccg acgagcaaat gagcgccacg
     1081 gctgttcgcg taagctcgaa acgacggaga ttcgatagag ccagagtcga gtttaaacga
     1141 ttcgggatct taaggataaa tttctggtaa ggaggacacg tatggaagtg ggcaagttgg
     1201 ggaagccgta tccgttgctg aatctggcat atgtgggagt ataagacgcg cagcgtcgca
     1261 tcaggcattt ttttctgcgc caatgcacct agaatcggtt accaatgctt aatcagtgag
     1321 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1381 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1441 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1501 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctatcaactg ttgccgggaa
     1561 gctagagtaa gtagttcgcc agttaatagt ttacgcaagg ttgttgccat tgctacaggc
     1621 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1681 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctcca
     1741 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1801 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga atactcaacc
     1861 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     1921 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa gcgttcttcg
     1981 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgc
     2041 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2101 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcatg
     2161 cgaaagcctc ctgctgatcc tgtctcttga tagatgtttg caaaagctag gactcgatta
     2221 tacgttgacc gatcagacta tcaatatcta ggtttaaaca gatcggtacg ctgccgtgac
     2281 caggcttgag tacaatatct gacgattgga cttcctgggt cccaagctgg attccgactg
     2341 gctgactgcc aagcaacgat atggatcgtc gagactcgca ctgagaggat cgttctcggt
     2401 acaccaggcg cgccccgatt tcgatattcc tcggatctcg gatagatcgg gattgaggat
     2461 gtaggttgaa cttctctcgg gtgatcccga tctcggatat tcgggattgg agtaatacct
     2521 ggcgataggc gctgccaagc aatagccaag gcgcaaggcc ggccaagaga cttgcgatag
     2581 ttcgttcgag aggtagcctg caagatggat cggacgaaag gtttgaagat tttccgacga
     2641 gggccttgga gagttcggaa atttaaatgc aactatggat gaacgaaata gacagatcgc
     2701 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2821 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3541 ttttgctcac atgatttaaa tgaaactcgt aatcggagaa cgggtgcaat agcaaggacc
     3601 agatcgaact tgactgacgc aattctgagc gtagtcgaaa cccagacaag tcgatctcgc
     3661 caagtagctg acttgactgg ttaattaacc atagagccca ccgcatcccc agcatgcctg
     3721 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
     3781 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
     3841 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
     3901 ctagaaggca cagggcttac atactagaag aactcgtcaa gaaggcgata gaaggcgatg
     3961 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     4021 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     4081 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     4141 aagcaggcat cgccgtgggt cacgacgaga tcctcgccgt cgggcatccg cgccttgagc
     4201 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     4261 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     4321 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     4381 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     4441 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     4501 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga gttcattcag ggcaccggac
     4561 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4621 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4681 gcgggagaac ctgcgtgcaa tccatcttgt tcaatcatgt tggctacctc ctttgcaaaa
     4741 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     4801 ctcggcctct gcataaataa aaaaaattag tcagcgatgg ggcggagaat gggcggaact
     4861 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     4921 gagatgcaga tcgcttaatt aagtttcgat agcccaaggt aaccaatgag attagggagg
     4981 gaagaaagtt tgatgaacta cttctgaccc ctagtggtgt ccagaaaaga ccattaaagg
     5041 aatgtcttga tcgaaatcga gagatcgcct gcaggatagc tgacgacctt aaggataaat
     5101 ttctggtaag gaggacacgt atggaagtgg gcaagttggg gaagccgtat ccgttgctga
     5161 atctggcata tgtgggagta taagacgcgc agcgtcgcat caggcatttt tttctgcgcc
     5221 aatgccggta gatagcaatc cggcccgagg ggcacaaaaa acccctcaag acccgtttag
     5281 aggccccaag gggttatgct acgataccga tagagatggg ccaataccac atttgtagag
     5341 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     5401 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5521 ctcatcaatg tatcttatca tgtctgtgag ctgaaggtac gctgtatctc agtcagtcaa
     5581 gctagacagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     5641 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     5701 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     5761 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca
     5821 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     5881 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     5941 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     6001 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     6061 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     6121 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattag
     6181 gcctgcggat ccagaaatcg attgtatcag tcagtcagtg caggaggaga caacttctag
     6241 aggatccttt atttacaaca agatccgcaa ctgcaacatc ctttcaagca ggaacaacag
     6301 ctagtcatac agcacaacat aattgtgacc ataacgatag ctataagtcc ggcgataaag
     6361 cccaaccaaa tgtaccaggg ccatttaata tattgttcgt atttgcccag ttcttgcaag
     6421 tcgatcaaag attcattcaa gttcttggcg acctcattaa ggcgatcaat ctctttctga
     6481 atgttgacga cacttgcatt tattcctgat atgtccccga gatcaacatc gggtgaagta
     6541 tgattcttaa agtatttatc caattcctct ttgaagctat ccaactcggg ttgcagggga
     6601 tcatataccg tgttgttcac gatcccaatg accacatcac agttgcctga gacaaatgta
     6661 ttgtcagtgg ttatgatttg gggttcatag aaattccttt gggtcacaaa ccaatgcgtg
     6721 ccattactga cgaaaacgcc ttccctggga aagtgcgctt tgccatcgtg acagattgct
     6781 ggagctgtcg taaagttctt ctcttgagca gggacataag tgacgtggag aaagaccact
     6841 ccatgtgggg cggattgggg gaaggacatc aagtgatagc ccttgccaca aaagtcaacc
     6901 cgtttggact gcccgagcac gcactctgac attttagtgg cggccaggtt ggcgcttgct
     6961 cgtatctcag cagcccggat caactgttgt gtcacataag tttggagtga ctggagcctg
     7021 cctgtgatga gccgatcaat ttgcacctcg gcttccacct tatcaagtct ggacaatatg
     7081 tcgttgagga cgctggagat tgcgccgaat ttagaactca gttgcttcac gagggtattc
     7141 agggcctggg cgttatgatt cacaacatct tggagtttgc cgagggcgga cgctgtgctt
     7201 gacagtgaat cttggatctt gccaatcgcg ctgttaaatt gattagcgat cagcttctga
     7261 ttttcataca atacattttg cgtgacacca atcccattga atctgtaagc catttgcata
     7321 gcgaaaggta tttgaagggc cgcacccgcg ccaaaagtcc agccggatgt aatggttcct
     7381 gccaacaatg ctgatgtata ctgtgcaatc atctcatcag tgagcagagg gggcagaact
     7441 gtcaacccat tgaatttttg agcacagata aggtcgcggg ccgcaatgtc tcccaaacaa
     7501 tccccatact gcttaatgaa acccgcgtca gccaacgtaa ctttgttgaa cagcagatcc
     7561 tcgataaagc tgcgttttga aggcttagat gggtctggca gtatttggct aaaattaaac
     7621 ccgccaaaat atttgattgg cggcgttttg tatatttgct ttacttgtgc gaacacctct
     7681 tgggtattct tgtcttgttc gaccgcaatt ccggtcaatg ctcgtttgag ttgagtacag
     7741 aaagacccgt attgaagcaa gaggttgcta cactcggtgg aatccccaca gatatacatc
     7801 gtacagtcta cgctcgtctt agtcatgctg acgggcaaga tttccgtggt aacgctaatg
     7861 gtaaagttag ttgggattgc gatggaattg tttgaataag ccacggagtt ctcagccccg
     7921 agactcatgg tataggcgat tatggactgg ctggctactg agcgagcgcg ccggtgtgat
     7981 ttcgtttgag tttgataaga cgcacagatt cctgccccaa taggaatatc acattcgtat
     8041 gaattattga catactcagc gccaatgaga caacccgctc tagtttgaaa gacattggaa
     8101 cctgttgagt agactctcca ggtgggtgtc agttggtcgg cgtgtatcgc cacagggact
     8161 tcggtacagt tgacaccttg atacaggaca gccacttggt tggatgtgtt cgttccggga
     8221 gttatcacgc tgaccccgcc gaagctacag ggtgttatat cgaggatttc cagtgtttgc
     8281 ggatcgcgga cggcatctgt ggtatcggca atgtctctac caaattgttg aaaagggaga
     8341 aatttcttgt ttgattctgt cagcacccct gttccggtga gcccattaaa gttgaagttc
     8401 acacatttat tcttcaccag gttagtgctt ttcttcggtc cgcaaactgt agccggtgcg
     8461 tggagcagct caaagctcaa gactaccacc cgatatggtt gatggcccac cccatatgtt
     8521 ggtcgaaatc cgtatgattg aaggggagaa taacagttag gccctgccac tccattacat
     8581 ggtttgttgc cgacttgata aatttcggtg gagatgtcac gttcaaaggg cttcagcttt
     8641 gacttgcgta acaggcggta aagataatta tagttgccgc tgggttttga atcgagctta
     8701 ttggagttcc atgctataac gcagccggtg aagtcatccg gcaacttata attatagtct
     8761 gctatatttc cggtttgccc cggtgcaatc tggctgactt cgttgccgcg tatgacgaaa
     8821 gaatctgcgt aaacgttggt aaaacacaag tcattcaatt tcgtagggct gaccccataa
     8881 cacttaaaag cgaagaatgg tgcgaaatta tagatgacac tataatctgc aacacaattt
     8941 gatattcgct tgcgattcca ggcataaact gatgcaaatg tagtggcatt aaatacttca
     9001 tggaaaggac acaaattggt gatgttaggg aatcgaacaa tggattcggt gggttggacg
     9061 cggaagttac tggtttggta tatgcctttc tcaactgtga aggacttgag tgtgcattta
     9121 gtttcggaca ggggatcgag ggcacagtct acggcatcgg taattgttcc gttttcgtta
     9181 tatttgagaa gaaaggtgcg tggttgcaaa tatcccacat agtaggctgc tgcgcccgct
     9241 gtccaaccac tactactgtc gacaggcgtg aggtagcttc gatgaagggc aagaagggtt
     9301 tggaaccggg tgatgttgat gccaatcggc aagtccacga ggggttccaa cgcggagaag
     9361 ccttgaggga gatcccgctc cagatttatc ggggtatgct tgctataaat tttgaagtag
     9421 ccgtctatat tcttaaacac aaattcgcgg agattcttga agttgccttc cttcccttca
     9481 agatccatga gaaatggttg ggaaacgtac tcaaaagtac agttgttagc ggaactgtaa
     9541 acgcggaact ctgactccat ccaagacttg ttattcttct gataaacatc gagaaaggga
     9601 tcgttacaaa attgaaattc acagactttg atgacgacat tggtggcgtt gttcacaatg
     9661 aggagagact gtgtcttgga gtccagggtg gtgccaaata tccatccccg gataatgttg
     9721 gacttctccg ttgaggcaaa gtatacgcca tcgttaaatg gcaaggcggg attatcaaat
     9781 cgtttagttc cgttggtgcc agaaacgtgg atagcatgga accaggtgac atttgagaag
     9841 aatggcagga aaaggtcttg agtagaatga aggacactgg acctaaagac tttatcggga
     9901 tagtatactc cacgagtaaa agagttcgtg taagactgcg ttctagttat gaggttcacg
     9961 cattgggaag aaacgagggg caggagaacg aggaacacaa acatggcggc cgcctttgcc
    10021 aaagtgatgg gccagcacac agaccagcac gttgcccagg agctgtggga ggaagataag
    10081 aggtatgaac atgattagca aaagggccta gcttggactc agaataatcc agccttatcc
    10141 caaccataaa ataaaagcag aatggtagct ggattgtagc tgctattagc aatatgaaac
    10201 ctcttacatc agttacaatt tatatgcaga aatatttata tgcagaaata ttgctattgc
    10261 cttaacccag aaattatcac tgttattctt tagaatggtg caaagaggca tgatacattg
    10321 tatcattatt gccctgaaag aaagagatta gggaaagtat tagaaataag ataaacaaaa
    10381 aagtatatta aaagaagaaa gcatttttta aaattacaaa tgcaaaatta ccctgatttg
    10441 gtcaatatgt
//
