LOCUS       sequence-429933-       10450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike for JN.4 pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 JN.4
FEATURES             Location/Qualifiers
     source          1..10450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    196..784
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(249..269)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(667..678)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    837..958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(848..867)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     902..921
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      1073..1119
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1149..1285
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1330..1401
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        1446..1642
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1493..1628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1656..2450
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1710..1729)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2320..2339
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2461..2685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2881..3469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2961..2980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(4215..4283,4284..5075)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4433..4452)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      5088..5224
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5376..5447
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5452..5498)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5516..5564
                     /note="synthetic polyadenylation signal"
     enhancer        5645..5948
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5950..6152
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6103..6123
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          6286..6761
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 acatttggtt gggctttatc gccggactta tagctatcgt tatggtcaca attatgttgt
       61 gctgtatgac tagctgttgt tcctgcttga aaggatgttg cagttgcgga tcttgttgta
      121 aataaaggat cctctagaag ttgtctcctc ctgcactgac tgactgatac aatcgatttc
      181 tggatccgca ggcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
      241 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
      301 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
      361 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
      421 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
      481 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
      541 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
      601 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
      661 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
      721 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
      781 ccgcctgtct agcttgactg actgagatac agcgtacctt cagctcacag acatgataag
      841 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      901 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      961 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1021 aagcaagtaa aacctctaca aatgtggtat tggcccatct ctatcggtat cgtagcataa
     1081 ccccttgggg cctctaaacg ggtcttgagg ggttttttgt gcccctcggg ccggattgct
     1141 atctaccggc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc
     1201 acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc
     1261 ctccttacca gaaatttatc cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt
     1321 cgatcaagac attcctttaa tggtcttttc tggacaccac taggggtcag aagtagttca
     1381 tcaaactttc ttccctccct aatctcattg gttaccttgg gctatcgaaa cttaattaag
     1441 cgatctgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     1501 cctaactccg cccagttccg cccattctcc gccccatcgc tgactaattt tttttattta
     1561 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     1621 tggaggccta ggcttttgca aaggaggtag ccaacatgat tgaacaagat ggattgcacg
     1681 caggttctcc cgccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     1741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     1801 tcaagaccga cctgtccggt gccctgaatg aactccagga cgaggcagcg cggctatcgt
     1861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     1921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     1981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     2161 aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc gtgacccacg
     2221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     2281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     2341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     2401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctag tatgtaagcc
     2461 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2521 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2581 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2641 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggttaat taaccagtca
     2701 agtcagctac ttggcgagat cgacttgtct gggtttcgac tacgctcaga attgcgtcag
     2761 tcaagttcga tctggtcctt gctattgcac ccgttctccg attacgagtt tcatttaaat
     2821 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2881 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3001 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3301 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcattta aatttccgaa
     3721 ctctccaagg ccctcgtcgg aaaatcttca aacctttcgt ccgatccatc ttgcaggcta
     3781 cctctcgaac gaactatcgc aagtctcttg gccggccttg cgccttggct attgcttggc
     3841 agcgcctatc gccaggtatt actccaatcc cgaatatccg agatcgggat cacccgagag
     3901 aagttcaacc tacatcctca atcccgatct atccgagatc cgaggaatat cgaaatcggg
     3961 gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt ctcgacgatc catatcgttg
     4021 cttggcagtc agccagtcgg aatccagctt gggacccagg aagtccaatc gtcagatatt
     4081 gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa acctagatat tgatagtctg
     4141 atcggtcaac gtataatcga gtcctagctt ttgcaaacat ctatcaagag acaggatcag
     4201 caggaggctt tcgcatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4261 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4321 atcagttggg tgcgcgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4381 agagttttcg ccccgaagaa cgctttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4441 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4501 ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga
     4561 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4621 ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4681 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4741 gtgacaccac gatgcctgta gcaatggcaa caaccttgcg taaactatta actggcgaac
     4801 tacttactct agcttcccgg caacagttga tagactggat ggaggcggat aaagttgcag
     4861 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4921 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4981 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5041 ctgagatagg tgcctcactg attaagcatt ggtaaccgat tctaggtgca ttggcgcaga
     5101 aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgccag attcagcaac
     5161 ggatacggct tccccaactt gcccacttcc atacgtgtcc tccttaccag aaatttatcc
     5221 ttaagatccc gaatcgttta aactcgactc tggctctatc gaatctccgt cgtttcgagc
     5281 ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat cgaatctcgt cagctatcgt
     5341 cagcttacct ttttggcagc gatcgcggct cccgacatct tggaccatta gctccacagg
     5401 tatcttcttc cctctagtgg tcataacagc agcttcagct acctctcaat tcaaaaaacc
     5461 cctcaagacc cgtttagagg ccccaagggg ttatgctatc aatcgttgcg ttacacacac
     5521 aaaaaaccaa cacacatcca tcttcgatgg atagcgattt tattatctaa ctgctgatcg
     5581 agtgtagcca gatctagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     5641 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     5701 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     5761 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     5821 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     5881 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     5941 ttaccatgct gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     6001 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     6061 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     6121 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atcgcctgga
     6181 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctcccct
     6241 cgaagcttac atgtggtacc gagctcggat cctgagaact tcagggtgag tctatgggac
     6301 ccttgatgtt ttctttcccc ttcttttcta tggttaagtt catgtcatag gaaggggaga
     6361 agtaacaggg tacacatatt gaccaaatca gggtaatttt gcatttgtaa ttttaaaaaa
     6421 tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt ccctaatctc
     6481 tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt ctaaagaata
     6541 acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat atttctgcat
     6601 ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa tccagctacc
     6661 attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca agctaggccc
     6721 ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc aacgtgctgg
     6781 tctgtgtgct ggcccatcac tttggcaaag gcggccgcca tgtttgtgtt cctcgttctc
     6841 ctgcccctcg tttcttccca atgcgtgatg ccgctgttta acctcataac tacaacgcag
     6901 tcttacacga actcttttac tcgtggagta tactatcccg ataaagtctt taggtccagt
     6961 gtccttcatc ttactcaaga ccttttcctg ccattcttct caaatgtcac ctggttccat
     7021 gctatctctg gcaccaacgg aactaaacga tttgataatc ccgtcttgcc atttaacgat
     7081 ggcgtatact ttgcctcaac ggagaagtcc aacattatcc ggggatggat atttggcacc
     7141 accctggact ccaagacaca gtctctcctc attgtgaaca acgccaccaa tgtcttcatc
     7201 aaagtctgtg aatttcaatt ttgtaacgat ccctttctcg atgtttatca taagaataac
     7261 aagtcttgga tggagtcaga gtccggcgtt tacagttccg ctaacaactg tacttttgag
     7321 tacgtttccc aaccatttct catggatctt gaagggaagc aaggcaactt caagaatctc
     7381 cgcgaatttg tgtttaagaa tatagacggc tacttcaaaa tttatagcaa gcataccccg
     7441 ataattggtc gggatttccc tcaaggcttc tccgcgttgg aacccctcgt ggacttgccg
     7501 attggcatca acatcacccg gttccaaacc cttcttgccc ttaatcgaag ctacctcacg
     7561 cctggcgaca gtagtagtgg ttggacagcg ggcgcagcag actactatgt gggatatttg
     7621 caaccacgca cctttcttct caaatataac gaaaacggaa caattaccga tgccgtagac
     7681 tgtgccctcg atcccctgtc cgaaactaaa tgcacactca agtccttcac agttgagaaa
     7741 ggcatatacc aaaccagtaa cttccgcgtc caacccaccg aatccattgt tcgattccct
     7801 aacgtcacca atttgtgtcc tttccatgaa gtatttaatg ccactcgatt tgcatcagtt
     7861 tatgcctgga atcgcacgcg aatatcaaat tgtgttgcag attatagtgt cctgtataat
     7921 ttcgcaccat tcttcgcttt taagtgttat ggggtcagcc ctacgaaatt gaatgacttg
     7981 tgttttacca acgtttacgc agattctttc gtcataaagg gcaacgaagt cagccagatt
     8041 gcaccggggc aaaccggaaa tatagcagac tataattata agttgccgga tgacttcacc
     8101 ggctgcgtta tagcatggaa ctccaataag ctcgattcaa aacacagcgg caactatgat
     8161 tattggtacc gcctgtttcg caagtcaaaa ctgaagccct ttgaacgtga catctccacc
     8221 gaaatttatc aagtcggcaa caaaccatgt aaaggaaagg ggcctaactg ttattttccc
     8281 cttcaatcat acggatttcg accaacatat ggggtgggcc atcaaccata tcgggtggta
     8341 gtcttgagct ttgagctgct ccacgcaccg gctacagttt gcggaccgaa gaaaagcact
     8401 aacctggtga agaataaatg tgtgaacttc aactttaatg ggctcaccgg aacaggggtg
     8461 ctgacaaaat caaacaagaa atttctccct tttcaacaat ttggtagaga cattgtcgat
     8521 accacagatg ccgtccgcga tccgcaaaca ctggaaatcc tcgatataac accctgtagc
     8581 ttcggcgggg tcagcgtgat aactcccgga acgaacacat ccaaccaagt ggctgtcctg
     8641 tatcaaggtg tcaactgtac cgaagtctct gtggcgatac acgccgacca actgacaccc
     8701 acctggagag tctactcaac aggttccaat gtctttcaaa ctagagcggg ttgtctcatt
     8761 ggcgctgagt atgtcaataa ttcatacgaa tgtgatattc ctattggggc aggaatctgt
     8821 gcgtcttatc aaactcaaac gaaatcacga cggcgcgctc gctcagtagc cagccagtcc
     8881 ataatcgcct ataccatgag tctcggggct gagaactccg tggcttattc aaacaattcc
     8941 atcgcaatcc caactaactt taccattagc gttaccacgg aaatcttgcc cgtcagcatg
     9001 actaagacga gcgtagactg tacgatgtat atctgtgggg attccaccga gtgtagcaac
     9061 ctcttgcttc aatacgggtc tttctgtact caactcaaac gagcattgac cggaattgcg
     9121 gtcgaacaag acaagaatac ccaagaggtg ttcgcacaag taaagcaaat atacaaaacg
     9181 ccgccaatca aatattttgg cgggtttaat tttagccaaa tactgccaga cccatctaag
     9241 ccttcaaaac gcagctttat cgaggatctg ctgttcaaca aagttacgtt ggctgacgcg
     9301 ggtttcatta agcagtatgg ggattgtttg ggagacattg cggcccgcga ccttatctgt
     9361 gctcaaaaat tcaatgggtt gacagttctg ccccctctgc tcactgatga gatgattgca
     9421 cagtatacat cagcattgtt ggcaggaacc attacatccg gctggacttt tggcgcgggt
     9481 gcggcccttc aaataccttt cgctatgcaa atggcttaca gattcaatgg gattggtgtc
     9541 acgcaaaatg tattgtatga aaatcagaag ctgatcgcta atcaatttaa cagcgcgatt
     9601 ggcaagatcc aagattcact gtttagcaca gcgtccgccc tcggcaaact ccaagatgtt
     9661 gtgaatcata acgcccaggc cctgaatacc ctcgtgaagc aactgagttc taaattcggc
     9721 gcaatctcca gcgtcctcaa cgacatattg tccagacttg ataaggtgga agccgaggtg
     9781 caaattgatc ggctcatcac aggcaggctc cagtcactcc aaacttatgt gacacaacag
     9841 ttgatccggg ctgctgagat acgagcaagc gccaacctgg ccgccactaa aatgtcagag
     9901 tgcgtgctcg ggcagtccaa acgggttgac ttttgtggca agggctatca cttgatgtcc
     9961 ttcccccaat ccgccccaca tggagtggtc tttctccacg tcacttatgt ccctgctcaa
    10021 gagaagaact ttacgacagc tccagcaatc tgtcacgatg gcaaagcgca ctttcccagg
    10081 gaaggcgttt tcgtcagtaa tggcacgcat tggtttgtga cccaaaggaa tttctatgaa
    10141 ccccaaatca taaccactga caatacattt gtctcaggca actgtgatgt ggtcattggg
    10201 atcgtgaaca acacggtata tgatcccctg caactcgagt tggatagctt caaagaggaa
    10261 ttggataaat actttaagaa tcatacttca cccgatgttg atctcgggga catatcagga
    10321 ataaatgcaa gtgtcgtcaa cattcagaaa gagattgatc gccttaatga ggtcgccaag
    10381 aacttgaatg aatctttgat cgacttgcaa gaactgggca aatacgaaca atatattaaa
    10441 tggccctggt
//
