LOCUS       sequence-429934-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike P521S for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 P521S (TCA)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(228..430)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(257..277)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        432..735
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    816..864
                     /note="synthetic polyadenylation signal"
     terminator      882..928
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    933..1004
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1156..1292)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1305..2096,2097..2165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1928..1947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2911..3499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3400..3419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3695..3919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3930..4724)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4041..4060)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4651..4670
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4738..4934)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4752..4887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    4979..5050
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5095..5231)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(5261..5307)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5422..5543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5459..5478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5513..5532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5596..6184
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5702..5713
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6111..6131
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          join(10081..10462,1..94)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atatgtgtac cctgttactt ctccccttcc tatgacatga acttaaccat agaaaagaag
       61 gggaaagaaa acatcaaggg tcccatagac tcaccctgaa gttctcagga tccgagctcg
      121 gtaccacatg taagcttcga ggggaggctg gatcggtccc ggtgtcttct atggaggtca
      181 aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaaccagc tctgcttata
      241 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      301 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      361 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      421 aaccgcatca gcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      481 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      541 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
      601 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
      661 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
      721 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
      781 tactagatct ggctacactc gatcagcagt tagataataa aatcgctatc catcgaagat
      841 ggatgtgtgt tggttttttg tgtgtgtaac gcaacgattg atagcataac cccttggggc
      901 ctctaaacgg gtcttgaggg gttttttgaa ttgagaggta gctgaagctg ctgttatgac
      961 cactagaggg aagaagatac ctgtggagct aatggtccaa gatgtcggga gccgcgatcg
     1021 ctgccaaaaa ggtaagctga cgatagctga cgagattcga tgcccgacga gcaaatgagc
     1081 gccacggctg ttcgcgtaag ctcgaaacga cggagattcg atagagccag agtcgagttt
     1141 aaacgattcg ggatcttaag gataaatttc tggtaaggag gacacgtatg gaagtgggca
     1201 agttggggaa gccgtatccg ttgctgaatc tggcatatgt gggagtataa gacgcgcagc
     1261 gtcgcatcag gcattttttt ctgcgccaat gcacctagaa tcggttacca atgcttaatc
     1321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat caactgttgc
     1561 cgggaagcta gagtaagtag ttcgccagtt aatagtttac gcaaggttgt tgccattgct
     1621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1741 cctccaatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgaatac
     1861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaagcgt
     1981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2041 actcgcgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2161 ctcatgcgaa agcctcctgc tgatcctgtc tcttgataga tgtttgcaaa agctaggact
     2221 cgattatacg ttgaccgatc agactatcaa tatctaggtt taaacagatc ggtacgctgc
     2281 cgtgaccagg cttgagtaca atatctgacg attggacttc ctgggtccca agctggattc
     2341 cgactggctg actgccaagc aacgatatgg atcgtcgaga ctcgcactga gaggatcgtt
     2401 ctcggtacac caggcgcgcc ccgatttcga tattcctcgg atctcggata gatcgggatt
     2461 gaggatgtag gttgaacttc tctcgggtga tcccgatctc ggatattcgg gattggagta
     2521 atacctggcg ataggcgctg ccaagcaata gccaaggcgc aaggccggcc aagagacttg
     2581 cgatagttcg ttcgagaggt agcctgcaag atggatcgga cgaaaggttt gaagattttc
     2641 cgacgagggc cttggagagt tcggaaattt aaatgcaact atggatgaac gaaatagaca
     2701 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2761 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2821 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3541 ctggcctttt gctcacatga tttaaatgaa actcgtaatc ggagaacggg tgcaatagca
     3601 aggaccagat cgaacttgac tgacgcaatt ctgagcgtag tcgaaaccca gacaagtcga
     3661 tctcgccaag tagctgactt gactggttaa ttaaccatag agcccaccgc atccccagca
     3721 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     3781 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     3841 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     3901 tggcaactag aaggcacagg gcttacatac tagaagaact cgtcaagaag gcgatagaag
     3961 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     4021 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     4081 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     4141 ttcggcaagc aggcatcgcc gtgggtcacg acgagatcct cgccgtcggg catccgcgcc
     4201 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     4261 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     4321 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     4381 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     4441 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     4501 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctggagttc attcagggca
     4561 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     4621 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     4681 caagcggcgg gagaacctgc gtgcaatcca tcttgttcaa tcatgttggc tacctccttt
     4741 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     4801 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag cgatggggcg gagaatgggc
     4861 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     4921 ctaattgaga tgcagatcgc ttaattaagt ttcgatagcc caaggtaacc aatgagatta
     4981 gggagggaag aaagtttgat gaactacttc tgacccctag tggtgtccag aaaagaccat
     5041 taaaggaatg tcttgatcga aatcgagaga tcgcctgcag gatagctgac gaccttaagg
     5101 ataaatttct ggtaaggagg acacgtatgg aagtgggcaa gttggggaag ccgtatccgt
     5161 tgctgaatct ggcatatgtg ggagtataag acgcgcagcg tcgcatcagg catttttttc
     5221 tgcgccaatg ccggtagata gcaatccggc ccgaggggca caaaaaaccc ctcaagaccc
     5281 gtttagaggc cccaaggggt tatgctacga taccgataga gatgggccaa taccacattt
     5341 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5401 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5521 tccaaactca tcaatgtatc ttatcatgtc tgtgagctga aggtacgctg tatctcagtc
     5581 agtcaagcta gacaggcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa
     5641 ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg
     5701 gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac
     5761 aggcgagcag ccatggaaag gacgtcagct tccccgacaa caccacggaa ttgtcagtgc
     5821 ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg
     5881 caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc
     5941 aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg
     6001 gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag
     6061 ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa
     6121 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
     6181 gattaggcct gcggatccag aaatcgattg tatcagtcag tcagtgcagg aggagacaac
     6241 ttctagagga tcctttattt acaacaagat ccgcaactgc aacatccttt caagcaggaa
     6301 caacagctag tcatacagca caacataatt gtgaccataa cgatagctat aagtccggcg
     6361 ataaagccca accaaatgta ccagggccat ttaatatatt gttcgtattt gcccagttct
     6421 tgcaagtcga tcaaagattc attcaagttc ttggcgacct cattaaggcg atcaatctct
     6481 ttctgaatgt tgacgacact tgcatttatt cctgatatgt ccccgagatc aacatcgggt
     6541 gaagtatgat tcttaaagta tttatccaat tcctctttga agctatccaa ctcgggttgc
     6601 aggggatcat ataccgtgtt gttcacgatc ccaatgacca catcacagtt gcctgagaca
     6661 aatgtattgt cagtggttat gatttggggt tcatagaaat tcctttgggt cacaaaccaa
     6721 tgcgtgccat tactgacgaa aacgccttcc ctgggaaagt gcgctttgcc atcgtgacag
     6781 attgctggag ctgtcgtaaa gttcttctct tgagcaggga cataagtgac gtggagaaag
     6841 accactccat gtggggcgga ttgggggaag gacatcaagt gatagccctt gccacaaaag
     6901 tcaacccgtt tggactgccc gagcacgcac tctgacattt tagtggcggc caggttggcg
     6961 cttgctcgta tctcagcagc ccggatcaac tgttgtgtca cataagtttg gagtgactgg
     7021 agcctgcctg tgatgagccg atcaatttgc acctcggctt ccaccttatc aagtctggac
     7081 aatatgtcgt tgaggacgct ggagattgcg ccgaagttag aactcagttg cttcacgagg
     7141 gtattcaggg cctgggcgtt ttgattcaca acatcttgga gtttgccgag ggcggacgct
     7201 gtgcttgaca gtgaatcttg gatcttgcca atcgcgctgt taaattgatt agcgatcagc
     7261 ttctgatttt catacaatac attttgcgtg acaccaatcc cattgaatct gtaagccatt
     7321 tgcatagcga aaggtatttg aagggccgca cccgcgccaa aagtccagcc ggatgtaatg
     7381 gttcctgcca acaatgctga tgtatactgt gcaatcatct catcagtgag cagagggggc
     7441 agaactgtca acccattgaa tttttgagca cagataaggt cgcgggccgc aatgtctccc
     7501 aaacaatccc catactgctt aatgaaaccc gcgtcagcca acgtaacttt gttgaacagc
     7561 agatcctcga taaagctgcg ttttgaaggc ttagatgggt ctggcagtat ttggctaaaa
     7621 ttaaacccgc caaaatcttt gattggcggc gttttgtata tttgctttac ttgtgcgaac
     7681 acctcttggg tattcttgtc ttgttcgacc gcaattccgg tcaatgctcg gttgagttga
     7741 gtacagaaag acccgtattg aagcaagagg ttgctacact cggtggaatc cccacagata
     7801 tacatcgtac agtctacgct cgtcttagtc atgctgacgg gcaagatttc cgtggtaacg
     7861 ctaatggtaa agttagttgg gattgcgatg gaattgtttg aataagccac ggagttctca
     7921 gccccgagac tcatggtata ggcgattatg gactggctgg ctactgagcg agcgcgccgt
     7981 ggtgagttcg tttgagtttg ataagacgca cagattcctg ccccaatagg aatatcacat
     8041 tcgtatgaat tattgacatg ctcagcgcca atgagacaac ccgctctagt ttgaaagaca
     8101 ttggaacctg ttgagtagac tctccaggtg ggtgtcagtt ggtcggcgtg tatcgccaca
     8161 gggacttcgg tacagttgac atcttgatac aggacagcca cttggttgga tgtgttcgtt
     8221 ccgggagtta tcacgctgac cccgccgaag ctacagggtg ttatatcgag gatttccagt
     8281 gtttgcggat cgcggacggc atctgtggta tcggcaatgt ctctaccaaa ttgttgaaaa
     8341 gggagaaatt tcttgtttga ttctgtcagc acccctgttc cggtgagccc attaaagttg
     8401 aagttcacac atttattctt caccaggtta gtgcttttct tcggtccgca aactgtagct
     8461 gatgcgtgga gcagctcaaa gctcaagact accacccgat atggttgata gcccacccca
     8521 tttgttggtt gaaatccgta tgattgaagg ggaaaataac agttaaaccc ttccactcca
     8581 ttacatgggg tgctgccagc ttgataaatt tcggtggaga tgtcacgttc aaagggcttc
     8641 agatttgact tgcgaaacag gcggtaaaga taattatagt tgccgccgac ttttgaatcg
     8701 agattattgg agttccatgc tataacgcag ccggtgaagt catccggcaa cttataatta
     8761 tagtctgcta tctttccggt ttgccccggt gcaatctgtc ggacttcgtc gccgcgtatg
     8821 acgaaagaat ctgcgtaaac gttggtaaaa cacaagtcat tcaatttcgt agggctgacc
     8881 ccataacact taaaagtgct gaatgatgcg ctattataca ggacactata atctgcaaca
     8941 caatttgata ttcgcttgcg attccaggca taaactgatg caaatcgagt ggcattaaat
     9001 acttcaccga aaggacacaa attggtgatg ttagggaatc gaacaatgga ttcggtgggt
     9061 tggacgcgga agttactggt ttggtatatg cctttctcaa ctgtgaagga cttgagtgtg
     9121 catttagttt cggacagggg atcgagggca cagtctacgg catcggtaat tgttccgttt
     9181 tcgttatatt tgagaagaaa ggtgcgtggt tgcaaatatc ccacatagta ggctgctgcg
     9241 cccgctgtcc aaccactact actgtcgcca ggcgtgaggt agcttcgatg aagggcaaga
     9301 agggtttgga accgggtgat gttgatgcca atcggcaagt ccacgagggg ttccaacgcg
     9361 gagaagcctt gagggagatc ccgaaccaga tttatcgggg tatgcttgct ataaattttg
     9421 aagtagccgt ctatattctt aaacacaaat tcgcggagat tcttgaagtt gccttgcttc
     9481 ccttcaagat ccatgagaaa tggttgggaa acgtactcaa aagtacagtt gttagcggaa
     9541 ctgtaaacgc ggaactctga ctccatccaa gacttgttat tcttatgata ataaaccccg
     9601 agaaagggat cgttacaaaa ttgaaattca cagactttga tgacgacatt ggtggcgttg
     9661 ttcacaatga ggagagactg tgtcttggag tccagggtgg tgccaaatat ccatccccgg
     9721 ataatgttgg acttctccgt tgaggcaaag tatacgccat cgttaaatgg caagacggga
     9781 ttatcaaatc gtttagttcc gttggtgcca gaaacgtgga tagcatggaa ccaggtgaca
     9841 tttgagaaga atggcaggaa aaggtcttga gtagaatgaa ggacactgga cctaaagact
     9901 ttatcgggat agtatactcc acgagtaaaa gagttcgtgt aagcgggtgg gagctgcgtt
     9961 ctagttgtga ggttcacgca ttgggaagaa acgaggggca ggagaacgag gaacacaaac
    10021 atggcggccg cctttgccaa agtgatgggc cagcacacag accagcacgt tgcccaggag
    10081 ctgtgggagg aagataagag gtatgaacat gattagcaaa agggcctagc ttggactcag
    10141 aataatccag ccttatccca accataaaat aaaagcagaa tggtagctgg attgtagctg
    10201 ctattagcaa tatgaaacct cttacatcag ttacaattta tatgcagaaa tatttatatg
    10261 cagaaatatt gctattgcct taacccagaa attatcactg ttattcttta gaatggtgca
    10321 aagaggcatg atacattgta tcattattgc cctgaaagaa agagattagg gaaagtatta
    10381 gaaataagat aaacaaaaaa gtatattaaa agaagaaagc attttttaaa attacaaatg
    10441 caaaattacc ctgatttggt ca
//
