LOCUS       sequence-429942-        6576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..6576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             633..659
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3003..3026
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3741..3862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3752..3771)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3806..3825
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    3966..4360
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(4374..4748)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        5341..5644
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5724..6312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5804..5823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6420..6468
                     /note="synthetic polyadenylation signal"
     misc_feature    6479..6570
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6519..6538
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgtacccata cgatgttcca gattacgctg
      661 gatccaaaat tgaagaaggc aaactggtga tttggattaa cggcgataaa ggctataacg
      721 gcctggcgga agtgggcaaa aaatttgaaa aagataccgg cattaaagtg accgtggaac
      781 atccggataa actggaagaa aaatttccgc aggtggcggc gaccggcgat ggcccggata
      841 ttattttttg ggcgcatgat cgctttggcg gctatgcgca gagcggcctg ctggcggaaa
      901 ttaccccgga taaagcgttt caggataaac tgtatccgtt tacctgggat gcggtgcgct
      961 ataacggcaa actgattgcg tatccgattg cggtggaagc gctgagcctg atttataaca
     1021 aagatctgct gccgaacccg ccgaaaacct gggaagaaat tccggcgctg gataaagaac
     1081 tgaaagcgaa aggcaaaagc gcgctgatgt ttaacctgca ggaaccgtat tttacctggc
     1141 cgctgattgc ggcggatggc ggctatgcgt ttaaatatga aaacggcaaa tatgatatta
     1201 aagatgtggg cgtggataac gcgggcgcga aagcgggcct gacctttctg gtggatctga
     1261 ttaaaaacaa acatatgaac gcggataccg attatagcat tgcggaagcg gcgtttaaca
     1321 aaggcgaaac cgcgatgacc attaacggcc cgtgggcgtg gagcaacatt gataccagca
     1381 aagtgaacta tggcgtgacc gtgctgccga cctttaaagg ccagccgagc aaaccgtttg
     1441 tgggcgtgct gagcgcgggc attaacgcgg cgagcccgaa caaagaactg gcgaaagaat
     1501 ttctggaaaa ctatctgctg accgatgaag gcctggaagc ggtgaacaaa gataaaccgc
     1561 tgggcgcggt ggcgctgaaa agctatgaag aagaactggc gaaagatccg cgcattgcgg
     1621 cgaccatgga aaacgcgcag aaaggcgaaa ttatgccgaa cattccgcag atgagcgcgt
     1681 tttggtatgc ggtgcgcacc gcggtgatta acgcggcgag cggccgccag accgtggatg
     1741 aagcgctgaa agatgcgcag accaacgaga acctttactt ccaagatctg tctaggacat
     1801 ctgtgctgcc agcccagatg atggccctga catttatcac ttacattggc tgcggtctgt
     1861 ctagcatttt cctgtccgtt actctggtga cctacattgc cttcgaaaag atcagaaggg
     1921 actatccctc taagatcctc atccagctgt gcgctgccct gcttctcctg aacctggttt
     1981 tccttctgga ctcctggatc gccctctaca agatgcaggg cctgtgtatc tctgtggctg
     2041 ttttcctgca ctactttctg ctcgtgtcct tcacttggat gggactggag gctttccaca
     2101 tgtacctggc cctggtgaag gtgttcaata catacatccg caaatatatc ctgaagttct
     2161 gcattgtggg ctggggcgtg cctgctgtgg tcgtgaccat tatcttgaca attagtccgg
     2221 ataattacgg tctgggctcc tatggtaagt ttccaaacgg gagccccgat gacttctgct
     2281 ggatcaacaa taacgctgta ttttatatca ccgttgtggg gtatttttgc gtgatctttc
     2341 tcctgaacgt ttccatgttc atcgtcgtgc tggtccaact ctgtcgtatc aaaaagaaaa
     2401 agcagctggg agcacagcgt aaaacttcaa tccaggacct gagatctatc gccggcctga
     2461 ccttcctcct ggggatcacc tggggctttg cctttttcgc atggggcccc gtcaacgtaa
     2521 cattcatgta cctgtttgcg atctttaaca cactgcaagg ttttttcatc ttcatcttct
     2581 actgcgtcgc caaagagaac gtccgtaagc agtggcgcag gtacctgtgc tgtggcaagc
     2641 tgcgcctcgc agagaactcc gactggtcca agacagcaac taacggtctg aagaaacaga
     2701 ctgtaaatca gggggtcagt tccagttcca actccctcca aagcagtagc aacagtacca
     2761 acagcaccac acttctcgtg aataacgact gcagtgttca cgccagcggc aatggaaacg
     2821 ctagcactga gagaaacggc gtttctttct ccgtgcagaa tggcgatgtt tgcctgcacg
     2881 actttacagg gaagcagcac atgttcaatg aaaaggaaga ttcctgtaac gggaaaggta
     2941 ggatggctct gagacgtacc tccaagaggg gtagcctgca cttcatcgag cagatgggtt
     3001 ctgactacaa agacgatgac gacaagtgaa gatctgacaa aactcacaca tgcccaccgt
     3061 gcccagcacc tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg
     3121 acaccctcat gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg
     3181 aagaccctga ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga
     3241 caaagccgcg ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc
     3301 tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc
     3361 cagcccccat cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt
     3421 acaccctgcc cccatcccgg gaggagatga ccaagaacca ggtcagcctg acctgcctgg
     3481 tcaaaggctt ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga
     3541 acaactacaa gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca
     3601 agctcaccgt ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc
     3661 acgaggctct gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaatgag
     3721 tgctagctgg ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3781 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3841 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3901 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggaatt
     3961 aattctaaaa tacagcatag caaaacttta acctccaaat caagcctcta cttgaatcct
     4021 tttctgaggg atgaataagg cataggcatc aggggctgtt gccaatgtgc attagctgtt
     4081 tgcagcctca ccttctttca tggagtttaa gatatagtgt attttcccaa ggtttgaact
     4141 agctcttcat ttctttatgt tttaaatgca ctgacctccc acattccctt tttagtaaaa
     4201 tattcagaaa taatttaaat acatcattgc aatgaaaata aatgtttttt attaggcaga
     4261 atccagatgc tcaaggccct tcataatatc ccccagttta gtagttggac ttagggaaca
     4321 aaggaacctt taatagaaat tggacagcaa gaaagcgagc ttctagctta tcctcagtcc
     4381 tgctcctctg ccacaaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
     4441 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
     4501 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
     4561 agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
     4621 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc
     4681 cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac
     4741 ttggccatga tggctcctcc tgtcaggaga ggaaagagaa gaaggttagt acaattgcta
     4801 tagtgagttg tattatacta tgcagatata ctatgccaat gattaattgt caaactaggg
     4861 ctgcagggtt catagtgcca cttttcctgc actgccccat ctcctgccca ccctttccca
     4921 ggcatagaca gtcagtgact taccaaactc acaggaggga gaaggcagaa gcttgagaca
     4981 gacccgcggg accgccgaac tgcgagggga cgtggctagg gcggcttctt ttatggtgcg
     5041 ccggccctcg gaggcagggc gctcggggag gcctagcggc caatctgcgg tggcaggagg
     5101 cggggccgaa ggccgtgcct gaccaatccg gagcacatag gagtctcagc cccccgcccc
     5161 aaagcaaggg gaagtcacgc gcctgtagcg ccagcgtgtt gtgaaatggg ggcttggggg
     5221 ggttggggcc ctgactagtc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     5281 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcat
     5341 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     5401 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     5461 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     5521 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     5581 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     5641 aacgcctgca ggttaattaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6361 aactcacgtt aagggatttt ggtcatggct agttaattaa catttaaatc agcggccgca
     6421 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgtaactaa
     6481 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
     6541 gtgcaagtgc aggtgccaga acatttctct atcgaa
//
