LOCUS       sequence-429944-        6489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..6489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             633..659
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2916..2939
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3654..3775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3665..3684)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3719..3738
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    3879..4273
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(4287..4661)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        5254..5557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5637..6225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5717..5736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6333..6381
                     /note="synthetic polyadenylation signal"
     misc_feature    6392..6483
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6432..6451
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgtacccata cgatgttcca gattacgctg
      661 gatccaaaat tgaagaaggc aaactggtga tttggattaa cggcgataaa ggctataacg
      721 gcctggcgga agtgggcaaa aaatttgaaa aagataccgg cattaaagtg accgtggaac
      781 atccggataa actggaagaa aaatttccgc aggtggcggc gaccggcgat ggcccggata
      841 ttattttttg ggcgcatgat cgctttggcg gctatgcgca gagcggcctg ctggcggaaa
      901 ttaccccgga taaagcgttt caggataaac tgtatccgtt tacctgggat gcggtgcgct
      961 ataacggcaa actgattgcg tatccgattg cggtggaagc gctgagcctg atttataaca
     1021 aagatctgct gccgaacccg ccgaaaacct gggaagaaat tccggcgctg gataaagaac
     1081 tgaaagcgaa aggcaaaagc gcgctgatgt ttaacctgca ggaaccgtat tttacctggc
     1141 cgctgattgc ggcggatggc ggctatgcgt ttaaatatga aaacggcaaa tatgatatta
     1201 aagatgtggg cgtggataac gcgggcgcga aagcgggcct gacctttctg gtggatctga
     1261 ttaaaaacaa acatatgaac gcggataccg attatagcat tgcggaagcg gcgtttaaca
     1321 aaggcgaaac cgcgatgacc attaacggcc cgtgggcgtg gagcaacatt gataccagca
     1381 aagtgaacta tggcgtgacc gtgctgccga cctttaaagg ccagccgagc aaaccgtttg
     1441 tgggcgtgct gagcgcgggc attaacgcgg cgagcccgaa caaagaactg gcgaaagaat
     1501 ttctggaaaa ctatctgctg accgatgaag gcctggaagc ggtgaacaaa gataaaccgc
     1561 tgggcgcggt ggcgctgaaa agctatgaag aagaactggc gaaagatccg cgcattgcgg
     1621 cgaccatgga aaacgcgcag aaaggcgaaa ttatgccgaa cattccgcag atgagcgcgt
     1681 tttggtatgc ggtgcgcacc gcggtgatta acgcggcgag cggccgccag accgtggatg
     1741 aagcgctgaa agatgcgcag accaacgaga acctttactt ccaaacattc aagaaagact
     1801 accaataccc taaaagtctt gacatcctgt ctaacgtcgg ttgtgccctg agcgtcacag
     1861 ggctcgcgtt gacggttatt tttcagatcg ttacccgcaa ggtgagaaag acctccgtga
     1921 catgggttct cgtcaacctc tgcatctcta tgctgatctt caacttgctg ttcgtgtttg
     1981 ggatcgagaa ttccaataag aacctgcaga cctccgatgg agatatcaat aacattgatt
     2041 tcgacaacaa tgatattccc aggaccgata caattaatat ccccaacccc atgtgtacgg
     2101 cgatcgccgc tctgctccac tattttcttc tggtaacctt cacttggaac gctctttccg
     2161 cagctcagtt gtattacctt ctgatccgca caatgaagcc tctgccgcgc catttcatcc
     2221 tgttcattag tctgatcgga tggggagtac ccgctatcgt agtggcaatt actgtcggag
     2281 taatctactc tcaaaacgga aacaatcctc aatgggagct ggactaccgt caggaaaaaa
     2341 tctgctggct ggcaatcccg gaacctaacg gggtgatcaa gagtcctctc ctgtggtcat
     2401 tcatcgtgcc tgtgaccatc attctgatta gcaacgtggt catgttcatc acaatcagca
     2461 tcaaagtgct ctggaagaat aaccagaact tgactagcac taaaaaggtg tcctctatga
     2521 aaaagattgt gtccacactg tctgtggcgg ttgtcttcgg catcacatgg atcctcgcct
     2581 atctgatgct ggtgaatgac gattctatcc gcatcgtgtt ctcctacatt ttctgccttt
     2641 tcaacacaac ccagggcctg cagatcttta tcctttacac tgtgcgcaca aaggtcttcc
     2701 agagcgaagc aagcaaggtt ttgatgctcc tgagttccat tggtaggcgc aaaagcctgc
     2761 ccagcgtgac tagaccccgc ctgcgcgtca agatgtacaa ctttctccgc agcttgccta
     2821 cactgcatga gcgctttcgc ctcttggaga ccagtccctc cactgaagag attaccctga
     2881 gcgaaagtga caacgctaag gagtctatcg gttctgacta caaagacgat gacgacaagt
     2941 gaagatctga caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac
     3001 cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg
     3061 aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt
     3121 acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca
     3181 gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg
     3241 agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca
     3301 aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggaggaga
     3361 tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
     3421 ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc
     3481 tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc
     3541 agcaggggaa cgtcttctca tgctccgtga tgcacgaggc tctgcacaac cactacacgc
     3601 agaagagcct ctccctgtct ccgggtaaat gagtgctagc tggccagaca tgataagata
     3661 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3721 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3781 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     3841 caagtaaaac ctctacaaat gtggtatgga attaattcta aaatacagca tagcaaaact
     3901 ttaacctcca aatcaagcct ctacttgaat ccttttctga gggatgaata aggcataggc
     3961 atcaggggct gttgccaatg tgcattagct gtttgcagcc tcaccttctt tcatggagtt
     4021 taagatatag tgtattttcc caaggtttga actagctctt catttcttta tgttttaaat
     4081 gcactgacct cccacattcc ctttttagta aaatattcag aaataattta aatacatcat
     4141 tgcaatgaaa ataaatgttt tttattaggc agaatccaga tgctcaaggc ccttcataat
     4201 atcccccagt ttagtagttg gacttaggga acaaaggaac ctttaataga aattggacag
     4261 caagaaagcg agcttctagc ttatcctcag tcctgctcct ctgccacaaa gtgcacgcag
     4321 ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc
     4381 atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac
     4441 agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc
     4501 tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc
     4561 acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg
     4621 cgcgcggtga gcaccggaac ggcactggtc aacttggcca tgatggctcc tcctgtcagg
     4681 agaggaaaga gaagaaggtt agtacaattg ctatagtgag ttgtattata ctatgcagat
     4741 atactatgcc aatgattaat tgtcaaacta gggctgcagg gttcatagtg ccacttttcc
     4801 tgcactgccc catctcctgc ccaccctttc ccaggcatag acagtcagtg acttaccaaa
     4861 ctcacaggag ggagaaggca gaagcttgag acagacccgc gggaccgccg aactgcgagg
     4921 ggacgtggct agggcggctt cttttatggt gcgccggccc tcggaggcag ggcgctcggg
     4981 gaggcctagc ggccaatctg cggtggcagg aggcggggcc gaaggccgtg cctgaccaat
     5041 ccggagcaca taggagtctc agccccccgc cccaaagcaa ggggaagtca cgcgcctgta
     5101 gcgccagcgt gttgtgaaat gggggcttgg gggggttggg gccctgacta gtcaaaacaa
     5161 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     5221 acgcccattg atgtactgcc aaaaccgcat catcatggta atagcgatga ctaatacgta
     5281 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     5341 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     5401 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     5461 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     5521 tcagccaggc gggccattta ccgtaagtta tgtaacgcct gcaggttaat taagaacatg
     5581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6061 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6301 gctagttaat taacatttaa atcagcggcc gcaataaaat atctttattt tcattacatc
     6361 tgtgtgttgg ttttttgtgt gaatcgtaac taacatacgc tctccatcaa aacaaaacga
     6421 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
     6481 tctatcgaa
//
