LOCUS       sequence-429972-       10788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cal-ID variant with T195/G196 split site, mammalian expression and 
            lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Kim JW, Yong AJH, Aisenberg EE, Lobel JH, Wang W, Dawson TM, Dawson 
            VL, Gao R, Jan YN, Bateup HS, Ingolia NT
  TITLE     Molecular recording of calcium signals via calcium-dependent 
            proximity labeling.
  JOURNAL   Nat Chem Biol. 2024 Jul;20(7):894-905. doi: 
            10.1038/s41589-024-01603-7. Epub 2024 Apr 24.
  PUBMED    38658655
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01603-7"; journalName: "Nat Chem Biol"; date: 
            "2024-07"; volume: "20"; issue: "7"; pages: "894-905"
COMMENT     Sequence Label: pNTI823
FEATURES             Location/Qualifiers
     source          1..10788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(17..605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(97..116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(776..1567,1568..1636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1637..1741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1816..1835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2007..2386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2388..2586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2541..2561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2604..2784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2831..2956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3449..3682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3867..3911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4209..4326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4391..5602
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5439..5458
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5682..5738
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             join(5739..5747,5748..5762)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     misc_feature    7249..7837
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7302..7322)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7720..7731)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7840..7856)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7841..7857)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8362..8542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8568..8585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8574..8798
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8844..9272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8931..8950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9141..9162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9281..9301)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9286..9615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9466..9601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9528..9547
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9663..9710
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9729..10103
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10233..10354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10270..10289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10324..10343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10403..10419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10403..10419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10416..10438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10427..10443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10451..10457,10458..10475,10476..10481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10496..10517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10634..10651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
       61 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
      961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1741 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     1801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     1861 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1921 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1981 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     2041 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2101 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2161 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2221 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2281 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2341 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2401 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2461 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2521 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2581 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2761 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2821 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2881 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2941 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     3001 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     3061 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     3121 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3181 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3241 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     3301 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     3361 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     3421 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     3481 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     3541 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     3601 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     3661 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     3721 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     3781 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     3841 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3901 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3961 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     4021 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     4081 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     4141 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     4201 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4261 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4321 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg
     4381 ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     4441 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     4501 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     4561 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     4621 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     4681 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     4741 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     4801 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     4861 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     4921 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     4981 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     5041 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     5101 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     5161 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     5221 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     5281 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     5341 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     5401 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     5461 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     5521 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     5581 gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatga
     5641 acgacatctt cgaggctcag cgtatcgaat ggcactcatc acgtcgtaag tggaataaga
     5701 caggtcacgc agtcagagct ataggtcggc tgagctcaga tctgtacgac gatgacgata
     5761 agctcgaggg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5821 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     5881 tggacaggaa tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt
     5941 tcgagcagga aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca
     6001 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6061 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6121 aaatcagtct gcggtctgcc gaaaagctgc agggcggtac cggagggagc aaagacaata
     6181 ctgtgcctct gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc
     6241 tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg
     6301 actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc
     6361 cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc
     6421 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
     6481 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
     6541 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
     6601 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
     6661 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
     6721 gcatcctggt ggagctggcc ggaataacac tgccggacca actgactgaa gagcagatcg
     6781 cagaatttaa agaggctttc tccctatttg acaaggacgg ggatgggaca ataacaacca
     6841 aggagctggg gacggtgatg cggtctctgg ggcagaaccc cacagaagca gagctgcagg
     6901 acatgatcaa tgaagtagat gccgacggtg acggcacaat cgacttccct gagttcctga
     6961 caatgatggc aagaaaaatg aaatacaggg acacggaaga agaaattaga gaagcgttcg
     7021 gtgtgtttga taaggatggc aatggctaca tcagtgcagc agagcttcgc cacgtgatga
     7081 caaaccttgg agagaagtta acagatgaag aggttgatga aatgatcagg gaagcagaca
     7141 tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca gcgaagtgag
     7201 gatccaccgg tgctagcagt taacgaattc gatatcaagc ttatcgataa tcaacctctg
     7261 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7321 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7381 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7441 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7501 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7561 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7621 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7681 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7741 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7801 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     7861 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7921 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7981 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8041 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     8101 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     8161 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     8221 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     8281 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     8341 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8401 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8461 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8521 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     8581 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     8641 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     8701 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     8761 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     8821 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     9061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9241 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     9301 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9361 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9421 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9481 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9541 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9601 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     9661 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     9721 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     9781 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     9841 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     9901 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     9961 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
    10021 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
    10081 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
    10141 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    10201 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    10261 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10321 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    10381 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10441 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10501 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10561 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaagg
//
