LOCUS       sequence-429975-        5361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cpTurboID (V5) for AAV production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Kim JW, Yong AJH, Aisenberg EE, Lobel JH, Wang W, Dawson TM, Dawson 
            VL, Gao R, Jan YN, Bateup HS, Ingolia NT
  TITLE     Molecular recording of calcium signals via calcium-dependent 
            proximity labeling.
  JOURNAL   Nat Chem Biol. 2024 Jul;20(7):894-905. doi: 
            10.1038/s41589-024-01603-7. Epub 2024 Apr 24.
  PUBMED    38658655
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01603-7"; journalName: "Nat Chem Biol"; date: 
            "2024-07"; volume: "20"; issue: "7"; pages: "894-905"
COMMENT     Sequence Label: pNTI826
FEATURES             Location/Qualifiers
     source          1..5361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        181..628
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      663..672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(672..680,681..695)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1683..1724
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1752..2340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1805..1825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2223..2234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2382..2399)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2388..2595
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2624..2764
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2624..2753
                     /label=AAV2 ITR
     rep_origin      2839..3294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2926..2945)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3136..3157
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3311..3330)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3430..3452
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3490..3508)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3576..3680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3681..3749,3750..4541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4712..5300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5201..5220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cgggctgcag gtcgactcta
      661 gagccaccat ggatctgtac gacgatgacg ataagctcga ggggagcgtg gcagtcctgc
      721 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
      781 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
      841 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
      901 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
      961 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
     1021 gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg
     1081 ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac
     1141 tgcaggaagc agggattaac ctggacagga atactctggc cgctacgctg atccgagagc
     1201 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacggtggg
     1261 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt
     1321 tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta
     1381 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctg cagggcggta
     1441 ccggagggag caaagacaat actgtgcctc tgaagctgat cgctctcctg gctaatggcg
     1501 agttccatag tggcgaacag ctgggagaaa ccctgggcat gtccagggcc gctatcaaca
     1561 agcacattca gactctgcgc gactggggcg tggacgtgtt caccgtgccc ggaaagggct
     1621 actctctgcc cgagcctatc ccgctgctga acgctaaaca gattctggga cagctggacg
     1681 gcggcaagcc catccccaac cccctgctgg gcctggacag cacctgagaa ttcgatatca
     1741 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     1801 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     1861 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     1921 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     1981 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2041 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2101 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2161 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2221 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2281 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2341 atcgataccg agcgctgctc gagctagagc tcgctgatca gcctcgactg tgccttctag
     2401 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     2461 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     2521 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2581 caggcatgct ggggaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     2641 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     2701 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     2761 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2821 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2881 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     2941 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3001 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     3061 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3121 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     3181 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     3241 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     3301 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3361 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3421 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3481 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3541 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3601 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3661 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3721 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3781 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3841 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3901 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3961 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4021 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4081 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4141 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4201 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4261 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4321 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4381 gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4441 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4501 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4561 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4621 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4921 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4981 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5041 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5101 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5161 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5221 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5281 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5341 gctggccttt tgctcacatg t
//
