LOCUS       sequence-429980-       10974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression 
            and lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Kim JW, Yong AJH, Aisenberg EE, Lobel JH, Wang W, Dawson TM, Dawson 
            VL, Gao R, Jan YN, Bateup HS, Ingolia NT
  TITLE     Molecular recording of calcium signals via calcium-dependent 
            proximity labeling.
  JOURNAL   Nat Chem Biol. 2024 Jul;20(7):894-905. doi: 
            10.1038/s41589-024-01603-7. Epub 2024 Apr 24.
  PUBMED    38658655
REFERENCE   2  (bases 1 to 10974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01603-7"; journalName: "Nat Chem Biol"; date: 
            "2024-07"; volume: "20"; issue: "7"; pages: "894-905"
COMMENT     Sequence Label: pNTI827
FEATURES             Location/Qualifiers
     source          1..10974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(17..605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(97..116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(776..1567,1568..1636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1637..1741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1816..1835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2007..2386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2388..2586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2541..2561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2604..2784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2831..2956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3449..3682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3867..3911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4209..4326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4391..5602
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5439..5458
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5682..5738
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             join(5739..5747,5748..5762)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             7197..7238
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7435..8023
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7488..7508)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7906..7917)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8026..8042)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8027..8043)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8548..8728
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8754..8771)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8760..8984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9030..9458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9117..9136)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9327..9348
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9467..9487)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9472..9801
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9652..9787
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9714..9733
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9849..9896
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9915..10289
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10419..10540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10456..10475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10510..10529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10589..10605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10589..10605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10602..10624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10613..10629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10637..10643,10644..10661,10662..10667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10682..10703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10820..10837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
       61 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
      961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1741 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     1801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     1861 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1921 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1981 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     2041 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2101 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2161 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2221 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2281 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2341 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2401 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2461 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2521 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2581 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2761 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2821 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2881 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2941 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     3001 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     3061 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     3121 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3181 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3241 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     3301 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     3361 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     3421 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     3481 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     3541 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     3601 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     3661 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     3721 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     3781 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     3841 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3901 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3961 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     4021 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     4081 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     4141 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     4201 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4261 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4321 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg
     4381 ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     4441 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     4501 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     4561 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     4621 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     4681 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     4741 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     4801 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     4861 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     4921 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     4981 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     5041 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     5101 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     5161 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     5221 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     5281 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     5341 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     5401 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     5461 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     5521 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     5581 gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatga
     5641 acgacatctt cgaggctcag cgtatcgaat ggcactcatc acgtcgtaag tggaataaga
     5701 caggtcacgc agtcagagct ataggtcggc tgagctcaga tctgtacgac gatgacgata
     5761 agctcgaggg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
     5821 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
     5881 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
     5941 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
     6001 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
     6061 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
     6121 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
     6181 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
     6241 ctctggccgc tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
     6301 gcctggctcc atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga
     6361 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
     6421 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
     6481 ggtctgccga aaagctgcag ggcggtaccg gagggagcaa agacaatact gtgcctctga
     6541 agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg ggagaaaccc
     6601 tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac tggggcgtgg
     6661 acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg ctgctgaacg
     6721 ctaaacagat tctgggacag ctggacggcc tgccggacca actgactgaa gagcagatcg
     6781 cagaatttaa agaggctttc tccctatttg acaaggacgg ggatgggaca ataacaacca
     6841 aggagctggg gacggtgatg cggtctctgg ggcagaaccc cacagaagca gagctgcagg
     6901 acatgatcaa tgaagtagat gccgacggtg acggcacaat cgacttccct gagttcctga
     6961 caatgatggc aagaaaaatg aaatacaggg acacggaaga agaaattaga gaagcgttcg
     7021 gtgtgtttga taaggatggc aatggctaca tcagtgcagc agagcttcgc cacgtgatga
     7081 caaaccttgg agagaagtta acagatgaag aggttgatga aatgatcagg gaagcagaca
     7141 tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca gcgaagggca
     7201 agcccatccc caaccccctg ctgggcctgg acagcaccgg cagcggctca aagatagcca
     7261 agccttcgga tactcttatc actactgtgg agtctaattc cagttggtgg accaactggg
     7321 tgatcccagc catctcagcc ctggctgtag ctctgatgta tcgcctctac atggcagaag
     7381 attgaggatc caccggtgct agcagttaac gaattcgata tcaagcttat cgataatcaa
     7441 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7501 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7561 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7621 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7681 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7741 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7801 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7861 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7921 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7981 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     8041 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     8101 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     8161 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8221 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     8281 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     8341 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     8401 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     8461 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     8521 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8581 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8641 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8701 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     8761 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8821 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8881 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8941 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     9001 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     9061 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9121 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9181 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9241 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9301 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9361 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9421 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     9481 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9541 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9601 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9661 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9721 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     9781 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     9841 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     9901 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     9961 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
    10021 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
    10081 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
    10141 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
    10201 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
    10261 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
    10321 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    10381 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    10441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10501 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    10561 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    10621 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10681 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    10741 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10801 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aagg
//
