LOCUS       sequence-429984-       10356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian 
            expression and lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Kim JW, Yong AJH, Aisenberg EE, Lobel JH, Wang W, Dawson TM, Dawson 
            VL, Gao R, Jan YN, Bateup HS, Ingolia NT
  TITLE     Molecular recording of calcium signals via calcium-dependent 
            proximity labeling.
  JOURNAL   Nat Chem Biol. 2024 Jul;20(7):894-905. doi: 
            10.1038/s41589-024-01603-7. Epub 2024 Apr 24.
  PUBMED    38658655
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01603-7"; journalName: "Nat Chem Biol"; date: 
            "2024-07"; volume: "20"; issue: "7"; pages: "894-905"
COMMENT     Sequence Label: pNTI935
FEATURES             Location/Qualifiers
     source          1..10356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(17..605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(97..116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(776..1567,1568..1636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1637..1741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1816..1835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2007..2386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2388..2586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2541..2561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2604..2784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2831..2956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3449..3682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3867..3911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4209..4326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4391..5602
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5439..5458
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5631..5640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5640..5648,5649..5663)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             6651..6692
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6817..7405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6870..6890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7288..7299)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7408..7424)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7409..7425)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7930..8110
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8136..8153)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8142..8366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8412..8840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8499..8518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8709..8730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8849..8869)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8854..9183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9034..9169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9096..9115
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9231..9278
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9297..9671
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9801..9922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9838..9857)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9892..9911
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9971..9987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9971..9987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9984..10006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9995..10011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10019..10025,10026..10043,10044..10049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10064..10085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10202..10219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
       61 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
      961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1741 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     1801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     1861 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1921 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1981 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     2041 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2101 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2161 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2221 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2281 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2341 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2401 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2461 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2521 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2581 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2761 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2821 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2881 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2941 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     3001 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     3061 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     3121 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3181 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3241 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     3301 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     3361 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     3421 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     3481 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     3541 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     3601 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     3661 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     3721 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     3781 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     3841 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3901 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3961 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     4021 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     4081 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     4141 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     4201 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4261 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4321 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg
     4381 ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     4441 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     4501 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     4561 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     4621 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     4681 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     4741 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     4801 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     4861 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     4921 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     4981 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     5041 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     5101 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     5161 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     5221 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     5281 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     5341 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     5401 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     5461 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     5521 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     5581 gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatgg
     5641 atctgtacga cgatgacgat aagctcgagg ggagcgtggc agtcctgcct gtggtcgact
     5701 ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat gcttgcattg
     5761 cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct ccttttggag
     5821 ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca atcggcctgg
     5881 gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca gacaaggtgc
     5941 gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc atcctggtgg
     6001 agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg attaacgtgg
     6061 ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg caggaagcag
     6121 ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg cgggcagccc
     6181 tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag aagctggata
     6241 acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc gggattagcc
     6301 gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc aaaccctgga
     6361 tgggcggaga aatcagtctg cggtctgccg aaaagctgca gggcggtacc ggagggagca
     6421 aagacaatac tgtgcctctg aagctgatcg ctctcctggc taatggcgag ttccatagtg
     6481 gcgaacagct gggagaaacc ctgggcatgt ccagggccgc tatcaacaag cacattcaga
     6541 ctctgcgcga ctggggcgtg gacgtgttca ccgtgcccgg aaagggctac tctctgcccg
     6601 agcctatccc gctgctgaac gctaaacaga ttctgggaca gctggacggc ggcaagccca
     6661 tccccaaccc cctgctgggc ctggacagca ccggcagcgg cagcaaaatg agtaaggatg
     6721 gaaagaaaaa gaaaaagaaa agcaaaacaa agtgtgtaat aatgtgagga tccaccggtg
     6781 ctagcagtta acgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     6841 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6901 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6961 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7021 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7081 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7141 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7201 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7261 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7321 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7381 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     7441 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7501 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7561 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7621 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7681 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     7741 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     7801 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     7861 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     7921 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7981 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8041 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8101 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8161 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8221 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8281 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8341 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     8401 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8461 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8521 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8581 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8641 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8701 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8761 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8821 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9001 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9121 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9181 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     9241 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     9301 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     9361 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     9421 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     9481 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     9541 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     9601 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     9661 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     9721 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     9781 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     9841 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9901 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     9961 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10021 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10081 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10141 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10201 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10261 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10321 caaaaggcca gcaaaaggcc aggaaccgta aaaagg
//
